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Registro Completo |
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Biblioteca(s): |
Embrapa Rondônia. |
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Data corrente: |
29/06/2023 |
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Data da última atualização: |
18/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, S. A.; MONDADORI, R. G.; NOLETO, G. S.; BARBOSA, I. P.; GONÇALVES, R. L.; GASPERIN, B. G.; ROVANI, M. T.; PAZ, E. F.; GOMES, L. S.; PFEIFER, L. F. M. |
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Afiliação: |
SAMIRA A. SILVA, UNIVERSIDADE FEDERAL DE PELOTAS; RAFAEL G. MONDADORI, UNIVERSIDADE FEDERAL DE PELOTAS; GABRIELLY S. NOLETO, UNIVERSIDADE FEDERAL DE RONDÔNIA; INGRID P. BARBOSA, UNIVERSIDADE FEDERAL DO ACRE; REUEL L. GONÇALVES, BIOGENESIS BAGO SAÚDE ANIMAL; BERNARDO G. GASPERIN, UNIVERSIDADE FEDERAL DE PELOTAS; MONIQUE T. ROVANI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; EANES F. PAZ, ASSITÊNCIA TÉCNICA E EXTENSÃO RURAL; LEONARDO S. GOMES, Embrapa Rondônia; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO. |
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Título: |
GnRH34 with or without estradiol cypionate in timed AI in Bos indicus beef cows. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Theriogenology, v. 209, p. 134-140, 2023. |
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ISSN: |
0093-691X |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2023.06.026 |
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Idioma: |
Inglês |
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Conteúdo: |
Two experiments were performed to evaluate the effects of GnRH treatment on the fertility of suckled Nelore beef cows treated with an estradiol/progesterone (E2/P4)-based protocol for timed artificial insemination (TAI). Experiment 1 focused on determining the effects of estradiol cypionate (EC) on ovulation in TAI cows treated with GnRH 34 h after removal of the intravaginal P4 device (IPD). Suckled cows (n ¼ 26) were treated with 2 mg estradiol benzoate (EB) and IPD containing 1 g P4. After 8 days, IPDs were removed, and all cows were treated with 150 mg of d-cloprostenol (prostaglandin F2 alpha analog) and 300 IU of equine chorionic gonadotropin (eCG), then separated into two treatment groups consisting of cows who received 1) saline 0.9% i.m. (GnRH34 group) or 2) 0.6 mg i.m. of EC (EC-GnRH34 group). On day 9 (05:00 p.m.), all cows were given GnRH (10.5 mg of buserelin acetate) i.m. No differences were observed between the groups (P > 0.05) in the time of ovulation after IPD removal or in the proportion of cows ovulating. Experiment 2 focused on determining the effects of GnRH34 along with or in the absence of EC on day 8 on pregnancy per AI (P/AI) in postpartum beef cows. Cows (n ¼ 981) were treated similarly to those in Experiment 1, but an additional group, the EC-GnRH48 group, was included, in which cows received EC on day 8 whereas those that did not show estrus received GnRH at TAI. Thus, in this experiment, groups consisted of GnRH34 (n ¼ 322), EC-GnRH34 (n ¼ 335), and EC-GnRH48 (n ¼ 324). A higher rate of estrus expression was observed in cows treated with EC following IPD removal (EC-GnRH34: 69%, EC-GnRH48: 64.8%) than in cows in the GnRH34 group (45.6%). No difference in P/AI was observed between the treatment groups (P ¼ 0.45), but P/AI in cows in the EC-GnRH34 group (64.2%) tended to be greater (P ¼ 0.1) than in cows in the GnRH34 group (58%). In summary, although ovulation synchrony did not differ among the groups, P/AI in cows treated with EC and GnRH 34 h after IPD removal tended to be greater than in cows treated solely with GnRH; this was most likely due to a shorter proestrus/estrus period, considering the lower proportion of cows that displayed estrus in the GnRH34 group. Finally, given that P/AI did not differ between the EC-GnRH34 and EC-GnRH48 groups, our results suggest that, for cows not displaying estrus, administration of EC at the time of IPD removal followed by treatment with GnRH 48 h afterward represents the most cost-efficient TAI strategy for South American Zebu-based beef operations. MenosTwo experiments were performed to evaluate the effects of GnRH treatment on the fertility of suckled Nelore beef cows treated with an estradiol/progesterone (E2/P4)-based protocol for timed artificial insemination (TAI). Experiment 1 focused on determining the effects of estradiol cypionate (EC) on ovulation in TAI cows treated with GnRH 34 h after removal of the intravaginal P4 device (IPD). Suckled cows (n ¼ 26) were treated with 2 mg estradiol benzoate (EB) and IPD containing 1 g P4. After 8 days, IPDs were removed, and all cows were treated with 150 mg of d-cloprostenol (prostaglandin F2 alpha analog) and 300 IU of equine chorionic gonadotropin (eCG), then separated into two treatment groups consisting of cows who received 1) saline 0.9% i.m. (GnRH34 group) or 2) 0.6 mg i.m. of EC (EC-GnRH34 group). On day 9 (05:00 p.m.), all cows were given GnRH (10.5 mg of buserelin acetate) i.m. No differences were observed between the groups (P > 0.05) in the time of ovulation after IPD removal or in the proportion of cows ovulating. Experiment 2 focused on determining the effects of GnRH34 along with or in the absence of EC on day 8 on pregnancy per AI (P/AI) in postpartum beef cows. Cows (n ¼ 981) were treated similarly to those in Experiment 1, but an additional group, the EC-GnRH48 group, was included, in which cows received EC on day 8 whereas those that did not show estrus received GnRH at TAI. Thus, in this experiment, groups consisted of GnRH34 (n ¼ 322), EC-GnRH34 (n ¼ 335),... Mostrar Tudo |
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Palavras-Chave: |
Amazônia Ocidental; Embrapa Rondôna; Western Amazon. |
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Thesagro: |
Bos Indicus; Campo Experimental; Estradiol; Fertilidade Animal; Gado Nelore; Hormônio; Ovulação; Prenhez; Reprodução Animal; Vaca de Corte. |
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Thesaurus Nal: |
Animal fertility; Animal reproduction; Beef cows; Demonstration farms; Hormones; Nellore; Ovulation; Pregnancy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154705/1/cpafro-18957.pdf
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Marc: |
LEADER 03947naa a2200505 a 4500
001 2154705
005 2023-07-18
008 2023 bl uuuu u00u1 u #d
022 $a0093-691X
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2023.06.026$2DOI
100 1 $aSILVA, S. A.
245 $aGnRH34 with or without estradiol cypionate in timed AI in Bos indicus beef cows.$h[electronic resource]
260 $c2023
520 $aTwo experiments were performed to evaluate the effects of GnRH treatment on the fertility of suckled Nelore beef cows treated with an estradiol/progesterone (E2/P4)-based protocol for timed artificial insemination (TAI). Experiment 1 focused on determining the effects of estradiol cypionate (EC) on ovulation in TAI cows treated with GnRH 34 h after removal of the intravaginal P4 device (IPD). Suckled cows (n ¼ 26) were treated with 2 mg estradiol benzoate (EB) and IPD containing 1 g P4. After 8 days, IPDs were removed, and all cows were treated with 150 mg of d-cloprostenol (prostaglandin F2 alpha analog) and 300 IU of equine chorionic gonadotropin (eCG), then separated into two treatment groups consisting of cows who received 1) saline 0.9% i.m. (GnRH34 group) or 2) 0.6 mg i.m. of EC (EC-GnRH34 group). On day 9 (05:00 p.m.), all cows were given GnRH (10.5 mg of buserelin acetate) i.m. No differences were observed between the groups (P > 0.05) in the time of ovulation after IPD removal or in the proportion of cows ovulating. Experiment 2 focused on determining the effects of GnRH34 along with or in the absence of EC on day 8 on pregnancy per AI (P/AI) in postpartum beef cows. Cows (n ¼ 981) were treated similarly to those in Experiment 1, but an additional group, the EC-GnRH48 group, was included, in which cows received EC on day 8 whereas those that did not show estrus received GnRH at TAI. Thus, in this experiment, groups consisted of GnRH34 (n ¼ 322), EC-GnRH34 (n ¼ 335), and EC-GnRH48 (n ¼ 324). A higher rate of estrus expression was observed in cows treated with EC following IPD removal (EC-GnRH34: 69%, EC-GnRH48: 64.8%) than in cows in the GnRH34 group (45.6%). No difference in P/AI was observed between the treatment groups (P ¼ 0.45), but P/AI in cows in the EC-GnRH34 group (64.2%) tended to be greater (P ¼ 0.1) than in cows in the GnRH34 group (58%). In summary, although ovulation synchrony did not differ among the groups, P/AI in cows treated with EC and GnRH 34 h after IPD removal tended to be greater than in cows treated solely with GnRH; this was most likely due to a shorter proestrus/estrus period, considering the lower proportion of cows that displayed estrus in the GnRH34 group. Finally, given that P/AI did not differ between the EC-GnRH34 and EC-GnRH48 groups, our results suggest that, for cows not displaying estrus, administration of EC at the time of IPD removal followed by treatment with GnRH 48 h afterward represents the most cost-efficient TAI strategy for South American Zebu-based beef operations.
650 $aAnimal fertility
650 $aAnimal reproduction
650 $aBeef cows
650 $aDemonstration farms
650 $aHormones
650 $aNellore
650 $aOvulation
650 $aPregnancy
650 $aBos Indicus
650 $aCampo Experimental
650 $aEstradiol
650 $aFertilidade Animal
650 $aGado Nelore
650 $aHormônio
650 $aOvulação
650 $aPrenhez
650 $aReprodução Animal
650 $aVaca de Corte
653 $aAmazônia Ocidental
653 $aEmbrapa Rondôna
653 $aWestern Amazon
700 1 $aMONDADORI, R. G.
700 1 $aNOLETO, G. S.
700 1 $aBARBOSA, I. P.
700 1 $aGONÇALVES, R. L.
700 1 $aGASPERIN, B. G.
700 1 $aROVANI, M. T.
700 1 $aPAZ, E. F.
700 1 $aGOMES, L. S.
700 1 $aPFEIFER, L. F. M.
773 $tTheriogenology$gv. 209, p. 134-140, 2023.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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Biblioteca |
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Origem |
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Cutter |
Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Trigo. |
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Data corrente: |
03/01/2018 |
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Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LIMA, J. O.; PEREIRA, J. F.; ARAÚJO, R. F. de; QUEIROZ, M. V. de. |
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Afiliação: |
JULIANA OLIVEIRA LIMA, UNIVERSIDADE FEDERAL DE VIÇOSA; JORGE FERNANDO PEREIRA, CNPGL; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA. |
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Título: |
Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, São Paulo, v. 48, n. 3, p. 602-606, 2017. |
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DOI: |
https://doi.org/10.1016/j.bjm.2016.12.009 |
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Idioma: |
Inglês |
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Conteúdo: |
Expression of pectinolytic genes is regulated by catabolic repression limiting the productionof pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here,we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose(DG) that show resistance to catabolite repression and overproduce PL. Three spontaneousand nine UV-induced mutants were obtained. Some mutants produced sectors (segmentsmorphologically different) that were also studied. The mutants were analyzed for pectinasesproduction on pectinase-agar plates and five mutants and two sectors showing larger clear-ing zones than the wild type were selected for quantitative assay. Although PL productionhigher than the wild type has been found, phenotype instability was observed for most ofthe mutants and, after transfers to nonselective medium, the DG resistance was no longerpresent. Only mutants M03 and M04 were stable maintaining the DG-resistance phenotype.When growing for 120 h in liquid medium containing glucose with or without pectin, bothmutants showed higher PL production. In the presence of glucose as sole carbon source, themutant M03 produced 7.8-fold more PL than the wild type. Due its phenotypic stability andPL overproduction, the mutant M03 presents potential for industrial applications. |
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Palavras-Chave: |
Pectinolytic complex; Spontaneous mutant; Ultraviolet mutagenesis. |
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Thesagro: |
Microbiologia; Mutação. |
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Thesaurus NAL: |
Microbiology; Mutation. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1084275/1/Pectin-lyase-overproduction-by-Penicillium-griseoroseum-mutants.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170232/1/ID44256-2017v48n4p602BrazJMicrobiol.pdf
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Marc: |
LEADER 02130naa a2200253 a 4500
001 2084275
005 2022-08-09
008 2017 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.bjm.2016.12.009$2DOI
100 1 $aLIMA, J. O.
245 $aPectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression.$h[electronic resource]
260 $c2017
520 $aExpression of pectinolytic genes is regulated by catabolic repression limiting the productionof pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here,we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose(DG) that show resistance to catabolite repression and overproduce PL. Three spontaneousand nine UV-induced mutants were obtained. Some mutants produced sectors (segmentsmorphologically different) that were also studied. The mutants were analyzed for pectinasesproduction on pectinase-agar plates and five mutants and two sectors showing larger clear-ing zones than the wild type were selected for quantitative assay. Although PL productionhigher than the wild type has been found, phenotype instability was observed for most ofthe mutants and, after transfers to nonselective medium, the DG resistance was no longerpresent. Only mutants M03 and M04 were stable maintaining the DG-resistance phenotype.When growing for 120 h in liquid medium containing glucose with or without pectin, bothmutants showed higher PL production. In the presence of glucose as sole carbon source, themutant M03 produced 7.8-fold more PL than the wild type. Due its phenotypic stability andPL overproduction, the mutant M03 presents potential for industrial applications.
650 $aMicrobiology
650 $aMutation
650 $aMicrobiologia
650 $aMutação
653 $aPectinolytic complex
653 $aSpontaneous mutant
653 $aUltraviolet mutagenesis
700 1 $aPEREIRA, J. F.
700 1 $aARAÚJO, R. F. de
700 1 $aQUEIROZ, M. V. de
773 $tBrazilian Journal of Microbiology, São Paulo$gv. 48, n. 3, p. 602-606, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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