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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
08/06/2020 |
Data da última atualização: |
12/06/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAMPESTRINI, L. H.; RASERA, G. B.; CAMARGO, A. C. de; FRANCHIN, M.; NANI, B. D.; ROSALEN, P. L.; CANNIATTI-BRAZACA, S. G.; BIASOTO, A. C. T.; SHAHIDIC, F.; ALENCAR, S. M. |
Afiliação: |
Luciano Henrique Campestrini; Gabriela Boscariol Rasera; Adriano Costa de Camargo; Marcelo Franchin; Bruno Dias Nani; Pedro Luiz Rosalen; Solange Guidolin Canniatti-Brazaca; ALINE TELLES BIASOTO MARQUES, CPATSA; Fereidoon Shahidic; Severino Matias Alencar. |
Título: |
Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Food Research International, v. 131, may 2020. |
DOI: |
https://doi.org/10.1016/j.foodres.2019.108532 |
Idioma: |
Inglês |
Conteúdo: |
Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains. MenosWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory c... Mostrar Tudo |
Palavras-Chave: |
Atividade anti-inflamatória; Extração sequencial; Polissacarídeos; Vinificação. |
Thesagro: |
Pectina; Subproduto; Uva. |
Thesaurus Nal: |
Grapes; Polysaccharides. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 02892naa a2200349 a 4500 001 2123132 005 2020-06-12 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.foodres.2019.108532$2DOI 100 1 $aCAMPESTRINI, L. H. 245 $aAlkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.$h[electronic resource] 260 $c2020 520 $aWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains. 650 $aGrapes 650 $aPolysaccharides 650 $aPectina 650 $aSubproduto 650 $aUva 653 $aAtividade anti-inflamatória 653 $aExtração sequencial 653 $aPolissacarídeos 653 $aVinificação 700 1 $aRASERA, G. B. 700 1 $aCAMARGO, A. C. de 700 1 $aFRANCHIN, M. 700 1 $aNANI, B. D. 700 1 $aROSALEN, P. L. 700 1 $aCANNIATTI-BRAZACA, S. G. 700 1 $aBIASOTO, A. C. T. 700 1 $aSHAHIDIC, F. 700 1 $aALENCAR, S. M. 773 $tFood Research International$gv. 131, may 2020.
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Embrapa Semiárido (CPATSA) |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
14/06/2010 |
Data da última atualização: |
01/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
NOBILE, P. M.; QUECINI, V.; BAZZO, B.; QUITERIO, G.; MAZZAFERA, P.; COLOMBO, C. A. |
Afiliação: |
PAULA M. NOBILE, IAC; VERA MARIA QUECINI, CNPUV; BARBARA BAZZO, IAC; GABRIELA QUITERIO, IAC; PAULO MAZZAFERA, INSTITUTO DE BIOLOGIA - UNICAMP; CARLOS A. COLOMBO, IAC. |
Título: |
Transcriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, Washington DC, v. 58, n. 6, p. 3479-3487, mar. 2010. |
DOI: |
10.1021/jf9043088 |
Idioma: |
Inglês |
Conteúdo: |
The present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage. MenosThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic back... Mostrar Tudo |
Palavras-Chave: |
Fator anti-nutricional; Ferritina; Fitato. |
Thesagro: |
Biologia; Café; Ferro; Genética. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204172/1/12303-2010-p.3479-3487-2.pdf
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Marc: |
LEADER 02404naa a2200277 a 4500 001 1854944 005 2019-11-01 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1021/jf9043088$2DOI 100 1 $aNOBILE, P. M. 245 $aTranscriptional profile of genes involved in the byosynthesis of phytate and ferritin in coffea.$h[electronic resource] 260 $c2010 520 $aThe present work aimed to study the control of the biosynthesis of the antinutritional factor phytate and its associated Fe-rich protein family, ferritin, in coffee. Phytate has the ability to chelate Fe, making it unavailable to human absorption. The Coffea genome databases were queried for genes associated with phytate metabolism and ferritin genes. The genetic framework for phytate biosynthesis and its reverse pathway was identified in silico analyses and indicate that Coffea phosphatidyl inositol kinase and monophosphatase families play nonredundant roles in phytate metabolism. The transcriptional profiles of phytate biosynthesis key-genes MYO-INOSITOL(3)P1 SYNTHASE, two genes coding for PHOSPHATIDYL INOSITOL KINASE, and three FERRITIN genes were temporally evaluated by qPCR in coffee seeds from two crop locations, Adamantina-SP and Ouro-Fino-MG, the last one traditionally associated with high-quality coffee beverage grain. A targeted metabolome profile of phytic acid contents throughout three fruit maturation stages in association with the transcriptional analysis was also obtained. Taken together, our data indicate that the investigated local conditions did not cause significant alterations in phytate biosynthesis. Futhermore, the temporal transcriptional profiling revealed that candidate gene expression is regulated independently of phytate accumulation. In contrast, the expression profile of ferritin-unit genes is affected by environmental conditions and genetic background. The roles of the investigated genes are discussed concerning the quality of coffee beverage. 650 $aBiologia 650 $aCafé 650 $aFerro 650 $aGenética 653 $aFator anti-nutricional 653 $aFerritina 653 $aFitato 700 1 $aQUECINI, V. 700 1 $aBAZZO, B. 700 1 $aQUITERIO, G. 700 1 $aMAZZAFERA, P. 700 1 $aCOLOMBO, C. A. 773 $tJournal of Agricultural and Food Chemistry, Washington DC$gv. 58, n. 6, p. 3479-3487, mar. 2010.
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