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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
23/12/2004 |
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Data da última atualização: |
21/08/2009 |
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Autoria: |
SOSA-GÓMEZ, D. R.; ROSADO NETO, G.; SILVA, J. J.; BORGES, E.; CORONEL, N.; SALAS, H. |
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Título: |
Sternechus pinguis, a missidentification of Sternechus subsignatus (Coleoptera: Curculionidae) in Argentina. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 20., 2004, Gramado. Programa e resumos. Gramado: Sociedade Entomológica do Brasil, 2004. |
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Páginas: |
p. 635. |
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Idioma: |
Inglês |
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Conteúdo: |
One of the most important curculionids causing yield losses in soybean fields belongs to the genus Sternechus. The species of this genus are distributed in the Neotropical region. In the last 14 years, several authors have cited the presence of S. pinguis in Argentina, while Brazilian papers cite S. subsignatus. At present, no record of S. pinguis have been found in Brazil. The purpose of this paper is to clarify the identify of the Argentinean and Brazilian specimens and determine molecular polymorphism at mtDNA level. Samples os Sternechus were taken to verify the identify trough the analysis of morphological characters and comparisons of fragments from the mtDNA. Samples of the putative S. subsignatus and S. pinguis were taken in December of 2003 and January and February of 2004, from Monte Redondo, (Tucumán, Argentina), Mauá da Serra (PR), Warta (PR), São Desiderio (BA) and Samambaiba (MA). The mt DNA was amplified by polymerase chain reactions using the following primers LR-J-12887-LR-N-13398, LCO1490-J1514-HCO2198-N-2175 and CYT BF-CYT- BR. PCR products were treated with restriction enzymes Rsa I, Hae III, Pst I, Bam HI, Sma I, Apa I, Hind III and Taq I. The PCR was performed using a PTC 200 thermal cycler (MJ Research) programmed for 34 cycles of 94oC for 1 min, 48 or 55oC 1 min (depending on the primer) and 72oC for 1:30 min with a final extension at 72oC for 5 min. The studies based on morphological characters suggest that specimens from Argentina belong to the same species found in Brazil. The lack of molecular polymorphism between the specimens in other evidence that the populations belong to the same species. MenosOne of the most important curculionids causing yield losses in soybean fields belongs to the genus Sternechus. The species of this genus are distributed in the Neotropical region. In the last 14 years, several authors have cited the presence of S. pinguis in Argentina, while Brazilian papers cite S. subsignatus. At present, no record of S. pinguis have been found in Brazil. The purpose of this paper is to clarify the identify of the Argentinean and Brazilian specimens and determine molecular polymorphism at mtDNA level. Samples os Sternechus were taken to verify the identify trough the analysis of morphological characters and comparisons of fragments from the mtDNA. Samples of the putative S. subsignatus and S. pinguis were taken in December of 2003 and January and February of 2004, from Monte Redondo, (Tucumán, Argentina), Mauá da Serra (PR), Warta (PR), São Desiderio (BA) and Samambaiba (MA). The mt DNA was amplified by polymerase chain reactions using the following primers LR-J-12887-LR-N-13398, LCO1490-J1514-HCO2198-N-2175 and CYT BF-CYT- BR. PCR products were treated with restriction enzymes Rsa I, Hae III, Pst I, Bam HI, Sma I, Apa I, Hind III and Taq I. The PCR was performed using a PTC 200 thermal cycler (MJ Research) programmed for 34 cycles of 94oC for 1 min, 48 or 55oC 1 min (depending on the primer) and 72oC for 1:30 min with a final extension at 72oC for 5 min. The studies based on morphological characters suggest that specimens from Argentina belong to the same... Mostrar Tudo |
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Thesagro: |
Curculionídeo; Inseto; Praga de Planta; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02391naa a2200241 a 4500
001 1467735
005 2009-08-21
008 2004 bl uuuu u00u1 u #d
100 1 $aSOSA-GÓMEZ, D. R.
245 $aSternechus pinguis, a missidentification of Sternechus subsignatus (Coleoptera$bCurculionidae) in Argentina.
260 $c2004
300 $ap. 635.
520 $aOne of the most important curculionids causing yield losses in soybean fields belongs to the genus Sternechus. The species of this genus are distributed in the Neotropical region. In the last 14 years, several authors have cited the presence of S. pinguis in Argentina, while Brazilian papers cite S. subsignatus. At present, no record of S. pinguis have been found in Brazil. The purpose of this paper is to clarify the identify of the Argentinean and Brazilian specimens and determine molecular polymorphism at mtDNA level. Samples os Sternechus were taken to verify the identify trough the analysis of morphological characters and comparisons of fragments from the mtDNA. Samples of the putative S. subsignatus and S. pinguis were taken in December of 2003 and January and February of 2004, from Monte Redondo, (Tucumán, Argentina), Mauá da Serra (PR), Warta (PR), São Desiderio (BA) and Samambaiba (MA). The mt DNA was amplified by polymerase chain reactions using the following primers LR-J-12887-LR-N-13398, LCO1490-J1514-HCO2198-N-2175 and CYT BF-CYT- BR. PCR products were treated with restriction enzymes Rsa I, Hae III, Pst I, Bam HI, Sma I, Apa I, Hind III and Taq I. The PCR was performed using a PTC 200 thermal cycler (MJ Research) programmed for 34 cycles of 94oC for 1 min, 48 or 55oC 1 min (depending on the primer) and 72oC for 1:30 min with a final extension at 72oC for 5 min. The studies based on morphological characters suggest that specimens from Argentina belong to the same species found in Brazil. The lack of molecular polymorphism between the specimens in other evidence that the populations belong to the same species.
650 $aCurculionídeo
650 $aInseto
650 $aPraga de Planta
650 $aSoja
700 1 $aROSADO NETO, G.
700 1 $aSILVA, J. J.
700 1 $aBORGES, E.
700 1 $aCORONEL, N.
700 1 $aSALAS, H.
773 $tIn: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 20., 2004, Gramado. Programa e resumos. Gramado: Sociedade Entomológica do Brasil, 2004.
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Registro original: |
Embrapa Soja (CNPSO) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
09/08/2022 |
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Data da última atualização: |
28/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FREITAS, C. D. de; OKI, Y.; RESENDE, F. M.; ZAMUDIO, F.; FREITAS, G. S. de; REZENDE, K. M. de; SOUZA, F. A. de; JONG, D. de; QUESADA, M.; CARVALHO, A. S.; PIRES, C. S. S.; FERNANDES, G. W. |
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Afiliação: |
CATARINA DIAS DE FREITAS, UFMG; YUMI OKI, UFMG; FERNANDO M. RESENDE, UFMG; FERNANDO ZAMUDIO, National University of Cordoba & CONICT, Argentina; GEUSA SIMONE DE FREITAS, USP; KEILA MOREIRA DE REZENDE, UFMG; FRANKLIN AMARO DE SOUZA, UFMG; DAVID DE JONG, USP; MAURICIO QUESADA, Universidade Nacional Autónoma de Mexico, Mexico; ANDRÉA SIQUEIRA CARVALHO, UFMG; CARMEN SILVIA SOARES PIRES, Cenargen; GERALDO WILSON FERNANDES, UFMG. |
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Título: |
Impacts of pests and diseases on the decline of managed bees in Brazil: a beekeeper perspective. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Apicultural Research, 2022. |
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DOI: |
https://doi.org/10.1080/00218839.2022.2099188 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Bee health; Insect decline; Participatory questionnaire; Social bees; Tingless bees. |
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Thesaurus NAL: |
Colony collapse disorder. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00972naa a2200325 a 4500
001 2145345
005 2023-09-28
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/00218839.2022.2099188$2DOI
100 1 $aFREITAS, C. D. de
245 $aImpacts of pests and diseases on the decline of managed bees in Brazil$ba beekeeper perspective.$h[electronic resource]
260 $c2022
650 $aColony collapse disorder
653 $aBee health
653 $aInsect decline
653 $aParticipatory questionnaire
653 $aSocial bees
653 $aTingless bees
700 1 $aOKI, Y.
700 1 $aRESENDE, F. M.
700 1 $aZAMUDIO, F.
700 1 $aFREITAS, G. S. de
700 1 $aREZENDE, K. M. de
700 1 $aSOUZA, F. A. de
700 1 $aJONG, D. de
700 1 $aQUESADA, M.
700 1 $aCARVALHO, A. S.
700 1 $aPIRES, C. S. S.
700 1 $aFERNANDES, G. W.
773 $tJournal of Apicultural Research, 2022.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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