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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
09/01/2023 |
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Data da última atualização: |
09/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FÁVARIS, N. A. B.; ROSA, S. D. V. F. da; FIGUEIREDO, M. A. de; COELHO, S. V. B.; VILELA, A. L. de O.; PADILHA, L. |
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Afiliação: |
NATHÁLIA APARECIDA BRAGANÇA FÁVARIS, UNIVERSIDADE FEDERAL DE LAVRAS; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; MADELEINE ALVES DE FIGUEIREDO, UNIVERSIDADE FEDERAL DE LAVRAS; STEFÂNIA VILAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; ANA LUIZA DE OLIVEIRA VILELA, UNIVERSIDADE FEDERAL DE LAVRAS; LILIAN PADILHA, CNPCa. |
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Título: |
Protein profile in arabica coffee seeds in electrophoresis gel: importance of freeze-drying. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Journal of Seed Science, v.44, e202244017, 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds are sensitive to desiccation and are used or stored with different moisture content values, which may affect the results of quality assessment. The aim of this study was to evaluate the changes in protein profile in electrophoresis gel in coffee seeds with different moisture content values under freeze-drying and without freeze-drying. Two lots of arabica coffee seeds were used, one of newly-harvested seeds and another of stored seeds. The seeds were dried to the moisture content values of 12, 15, 20, 25, 30, 35, and 40%. The physiological quality of the seeds was assessed through the germination test, electrical conductivity, and the profiles of the enzymes SOD, CAT, PO, GOT, MDH, and EST and of heat-resistant proteins. In general, there is an effect on expression of these enzymes in accordance with the presence of free water in the seeds. Moist seeds have little to no enzyme expression. The freeze-drying process allows preservation of coffee seed quality and does not change the functionality of the enzymes studied. The isoenzyme profiles of the antioxidant process in arabica coffee seeds are affected by the initial moisture content of the seeds. The freeze-drying process of the seeds ensures greater sensitivity in detection of the expression of these isoenzymes. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Enzymes; Freeze drying; Seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150809/1/Protein-profile-in-arabica-coffee-seeds.pdf
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Marc: |
LEADER 01968naa a2200229 a 4500
001 2150809
005 2023-01-09
008 2022 bl uuuu u00u1 u #d
100 1 $aFÁVARIS, N. A. B.
245 $aProtein profile in arabica coffee seeds in electrophoresis gel$bimportance of freeze-drying.$h[electronic resource]
260 $c2022
520 $aCoffee seeds are sensitive to desiccation and are used or stored with different moisture content values, which may affect the results of quality assessment. The aim of this study was to evaluate the changes in protein profile in electrophoresis gel in coffee seeds with different moisture content values under freeze-drying and without freeze-drying. Two lots of arabica coffee seeds were used, one of newly-harvested seeds and another of stored seeds. The seeds were dried to the moisture content values of 12, 15, 20, 25, 30, 35, and 40%. The physiological quality of the seeds was assessed through the germination test, electrical conductivity, and the profiles of the enzymes SOD, CAT, PO, GOT, MDH, and EST and of heat-resistant proteins. In general, there is an effect on expression of these enzymes in accordance with the presence of free water in the seeds. Moist seeds have little to no enzyme expression. The freeze-drying process allows preservation of coffee seed quality and does not change the functionality of the enzymes studied. The isoenzyme profiles of the antioxidant process in arabica coffee seeds are affected by the initial moisture content of the seeds. The freeze-drying process of the seeds ensures greater sensitivity in detection of the expression of these isoenzymes.
650 $aEnzymes
650 $aFreeze drying
650 $aSeeds
650 $aCoffea Arábica
700 1 $aROSA, S. D. V. F. da
700 1 $aFIGUEIREDO, M. A. de
700 1 $aCOELHO, S. V. B.
700 1 $aVILELA, A. L. de O.
700 1 $aPADILHA, L.
773 $tJournal of Seed Science$gv.44, e202244017, 2022.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 157 | |
| 2. |  | ROSA, S. D. V. F. da; COELHO, S. V. B; FIGUEIREDO, M. A. Avanços da pesquisa em armazenamento de sementes de café em temperaturas subzero. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 42., 2016, Serra Negra, SP. Produzir mais café, com economia, só com boa tecnologia: trabalhos apresentados. Varginha: Fundação Procafé, 2016. 466 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 5. | | VELOSO, J. S.; ROSA, S. D. V. F. da; PADILHA, L.; SCHENK, J. C. M. Molecular evaluation of coffee seeds under cryopreservation. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 129.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 157 | |
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