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Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
25/11/2022 |
Data da última atualização: |
25/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CARVALHO, M. H. de; ROSA, S. D. V. F. da; COELHO, S. V. B.; GUIMARÃES, C. C.; MARTINS, R. de S.; CLEMENTE, A. da C. S.; PAIVA, L. V. |
Afiliação: |
MAYARA HOLANDA DE CARVALHO; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; STEFÂNIA VIIAS BOAS COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; CRISTIANE CARVALHO GUIMARÃES, UNIVERSIDADE ESTADUAL PAULISTA; RAYANA DE SÁ MARTINS, INSTITUTO DE BOTÂNICA; ALINE DA CONSOLAÇÃO SAMPAIO CLEMENTE, ONSEEDS TREINAMENTOS; LUCIANO VILELA PAIVA, UNIVERSIDADE FEDERAL DE LAVRAS. |
Título: |
Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Acta Scientiarum: Agronomy, v. 45, e56908, 2023. |
DOI: |
https://doi.org/10.4025/actasciagron.v45i1.56908 |
Idioma: |
Inglês |
Conteúdo: |
The reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality. |
Thesaurus Nal: |
Coffea arabica var. arabica; Drying; Enzyme activity; Oxidative stress. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148771/1/Carvalho-et-al-2023-Drying-of-arabica-coffee-and-its-effect-on-the-gene-expression-and-activity-of-enzymes-linked-to-seed-physiological-quality.pdf
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Marc: |
LEADER 02045naa a2200253 a 4500 001 2148771 005 2022-11-25 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.4025/actasciagron.v45i1.56908$2DOI 100 1 $aCARVALHO, M. H. de 245 $aDrying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality.$h[electronic resource] 260 $c2023 520 $aThe reduced longevity of coffee seeds has been attributed to their sensitivity to desiccation. Studies related to gene expression and enzyme activity in coffee seeds under drying are important for understanding the effects of drying on their physiological quality. The aim of this study was to investigate the molecular aspects of seeds under different drying methods and associate them with physiological quality. Coffee seeds with different water contents were dried both slowly and rapidly. Enzymatic activity was analysed, as well as the expression of genes that encode the enzymes superoxide dismutase, catalase, peroxiredoxins, isocitrate lyase, and endo-ß-mannanase. There was a significant effect of drying speed and final water content on enzyme activity and on the expression of the different genes analysed. In seeds under rapid drying, there was greater expression of the genes that encode the enzymes catalase and endo-ß-mannanase. Greater expression of the 1 CYS PRX and SOD genes and greater activity of the ICL isoenzymes were found in seeds with superior physiological quality, but greater activity of the endo-β-mannanase and CAT enzymes occurred in seeds with lower physiological quality. 650 $aCoffea arabica var. arabica 650 $aDrying 650 $aEnzyme activity 650 $aOxidative stress 700 1 $aROSA, S. D. V. F. da 700 1 $aCOELHO, S. V. B. 700 1 $aGUIMARÃES, C. C. 700 1 $aMARTINS, R. de S. 700 1 $aCLEMENTE, A. da C. S. 700 1 $aPAIVA, L. V. 773 $tActa Scientiarum: Agronomy$gv. 45, e56908, 2023.
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Registro original: |
Embrapa Café (CNPCa) |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
22/05/2003 |
Data da última atualização: |
04/05/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ALZATE-MARIN, A. L.; COSTA, M. R.; SARTORATO, A.; DEL PELOSO, M. J.; BARROS, E. G. de; MOREIRA, M. A. |
Afiliação: |
ANA LILIA ALZATE-MARIN, UFV; MARCIA REGINA COSTA, UFV; ALOISIO SARTORATO, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; EVERALDO GONÇALVES DE BARROS, UFV; MAURILIO ALVES MOREIRA, UFV. |
Título: |
Genetic variability and pedigree analysis of brazilian common bean elite genotypes. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Scientia Agricola, v. 60, n. 2, p. 283-290, abr./jun. 2003. |
DOI: |
https://doi.org/10.1590/S0103-90162003000200012 |
Idioma: |
Inglês |
Conteúdo: |
Genetic diversity is essential for any breeding program. However, breeders tend to concentrate on specific genotypes, which combine traits of interest and may be used as progenitors in several breeding programs. Common bean (Phaseolus vulgaris L.) breeding programs are not different in this sense. In this study, the genetic diversity of 21 common bean elite lines from the Bean Regional Trials conducted by the Embrapa Rice and Bean Research Center was evaluated using the Random Amplified Polymorphic DNA (RAPD) and pedigree analyses. Based on genetic dissimilarity, three groups were defined: group I - lines 1, 9 and 10, with low genetic distances among them (0.00 to 0.06), originated from 11 Mesoamerican parents; group II - 17 lines with genetic distances ranging from 0.03 to 0.33, originated from 50 parents (mostly Mesoamerican); and group III - line 21 (PR 93201472), which parents are the Andean cultivar 'Pompadour' and the cultivar 'Irai' (unknown origin). The genetic distances between line 21 and the lines of the other two groups varied from 0.68 to 0.93. Pedigree analyses demonstrated that cultivars 'Carioca', 'Cornell 49-242', 'Jamapa', 'Tlalnepantla 64', 'Tara' and 'Veranic 2', all of Mesoamerican origin, were the most widely used parents for developing lines present in group II. |
Palavras-Chave: |
Cultivares andinos; Cultivares Mesoamericanos; Distancia; RAPD. |
Thesagro: |
Feijão; Genética; Marcador Molecular; Melhoramento; Phaseolus Vulgaris. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/72539/1/15330.pdf
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Marc: |
LEADER 02199naa a2200301 a 4500 001 1210999 005 2022-05-04 008 2003 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1590/S0103-90162003000200012$2DOI 100 1 $aALZATE-MARIN, A. L. 245 $aGenetic variability and pedigree analysis of brazilian common bean elite genotypes.$h[electronic resource] 260 $c2003 520 $aGenetic diversity is essential for any breeding program. However, breeders tend to concentrate on specific genotypes, which combine traits of interest and may be used as progenitors in several breeding programs. Common bean (Phaseolus vulgaris L.) breeding programs are not different in this sense. In this study, the genetic diversity of 21 common bean elite lines from the Bean Regional Trials conducted by the Embrapa Rice and Bean Research Center was evaluated using the Random Amplified Polymorphic DNA (RAPD) and pedigree analyses. Based on genetic dissimilarity, three groups were defined: group I - lines 1, 9 and 10, with low genetic distances among them (0.00 to 0.06), originated from 11 Mesoamerican parents; group II - 17 lines with genetic distances ranging from 0.03 to 0.33, originated from 50 parents (mostly Mesoamerican); and group III - line 21 (PR 93201472), which parents are the Andean cultivar 'Pompadour' and the cultivar 'Irai' (unknown origin). The genetic distances between line 21 and the lines of the other two groups varied from 0.68 to 0.93. Pedigree analyses demonstrated that cultivars 'Carioca', 'Cornell 49-242', 'Jamapa', 'Tlalnepantla 64', 'Tara' and 'Veranic 2', all of Mesoamerican origin, were the most widely used parents for developing lines present in group II. 650 $aFeijão 650 $aGenética 650 $aMarcador Molecular 650 $aMelhoramento 650 $aPhaseolus Vulgaris 653 $aCultivares andinos 653 $aCultivares Mesoamericanos 653 $aDistancia 653 $aRAPD 700 1 $aCOSTA, M. R. 700 1 $aSARTORATO, A. 700 1 $aDEL PELOSO, M. J. 700 1 $aBARROS, E. G. de 700 1 $aMOREIRA, M. A. 773 $tScientia Agricola$gv. 60, n. 2, p. 283-290, abr./jun. 2003.
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Embrapa Arroz e Feijão (CNPAF) |
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