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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
06/05/2019 |
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Data da última atualização: |
06/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, S. V. B.; ROSA, S. D. V. F. da; FANTAZZINI, T. B.; BAUTE, J. L.; SILVA, L. C. |
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Afiliação: |
Stefânia Vilas Boas Coelho, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Júlia Lima Baute, Departamento de Agricultura - DAG/Universidade Federal de Lavras- UFLA; Luciano Coutinho Silva, Departamento de Biologia Celular e Molecular/Universidade Federal da Paraíba - UFPB. |
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Título: |
Cryopreservation in Coffea canephora Pierre seeds: slow and fast cooling. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v. 42, n. 6, p. 588-597, Nov/Dec. 2018 |
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Idioma: |
Português |
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Notas: |
Título em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido. |
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Conteúdo: |
Coffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality. MenosCoffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Cat... Mostrar Tudo |
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Palavras-Chave: |
Cristal de gelo; Drying in silica gel; Ice crystals; Isoenzymes; Physiological quality; Qualidade fisiológica; Secagem em sílica gel; Semente recalcitrante. |
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Thesagro: |
Isoenzima; Secagem. |
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Thesaurus Nal: |
Recalcitrant seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/196979/1/Cryopreservation-in-Coffea-canephora-Pierre-seeds.pdf
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Marc: |
LEADER 02666naa a2200313 a 4500
001 2108759
005 2019-05-06
008 2018 bl uuuu u00u1 u #d
100 1 $aCOELHO, S. V. B.
245 $aCryopreservation in Coffea canephora Pierre seeds$bslow and fast cooling.$h[electronic resource]
260 $c2018
500 $aTítulo em português Criopreservação em sementes de Coffea canephora Pierre: Resfriamento lento e rápido.
520 $aCoffee is one of the main agricultural commodities in the country, and it is important to conservation of plant material for breeding programs. Cryopreservation is a promising alternative for preserving in the long-term the germplasm of species considered recalcitrant. However, studies should be performed to achieve maximum survival of seedlings after immersion in liquid nitrogen. The objective of this work was to find a cryopreservation protocol for storing seeds of Coffea canephora, studying two methods of cryopreservation, slow and fast cooling. Seeds were subjected to drying in silica gel up to the water content of 0.25 g g-1. In the first experiment, dried seeds were subjected to treatments of slow cooling at speeds of -1 ºC min-1,-3 ºC min-1 and -5 ºC min-1 until the end temperatures of -40 ºC, -50 ºC and -60 ºC, by means of a bio freezer and subsequently immersed in liquid nitrogen. In the second experiment, the best result was selected of the first experiment and compared with the rapid cooling, in which dried seeds, with 0.25 g g-1 of water content, were immersed directly into liquid nitrogen. Physiological and biochemical alterations occurring in the seeds after cryopreservation were evaluated. Coffea canephora seeds respond better to cryopreservation by rapid cooling, when compared to slow cooling. Drying, one of the cryopreservation steps does not affect the viability of Coffea canephora Pierre seeds, when these seeds are dried to 0.25 g g-1 of water content. Catalase and esterase enzymes are good biochemical markers for cryopreserved coffee seeds and their activity is greater in larger seed physiological quality.
650 $aRecalcitrant seeds
650 $aIsoenzima
650 $aSecagem
653 $aCristal de gelo
653 $aDrying in silica gel
653 $aIce crystals
653 $aIsoenzymes
653 $aPhysiological quality
653 $aQualidade fisiológica
653 $aSecagem em sílica gel
653 $aSemente recalcitrante
700 1 $aROSA, S. D. V. F. da
700 1 $aFANTAZZINI, T. B.
700 1 $aBAUTE, J. L.
700 1 $aSILVA, L. C.
773 $tCiência e Agrotecnologia$gv. 42, n. 6, p. 588-597, Nov/Dec. 2018
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 157 | |
| 44. |  | CARVALHO, A. M. de; ROSA, S. D. V. F. da; NOQUEIRA, F. D.; SANDY, E. C.; VEIGA, A. D. Formação de mudas de Coffea arábica L. a partir de sementes produzidas sob diferentes doses de fertilizantes nitrogenados. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 45. | | TAVEIRA, J. H. da S.; ROSA, S. D. V. F. da; BORÉM, F. M.; GIOMO, G. S.; SAATH, R. Perfis proteicos e desempenho fisiológico de sementes de café submetidas a diferentes métodos de processamento e secagem. PESQUISA AGROPECUÁRIA BRASILEIRA, Brasília, DF., V. 47, N. 10, P. 1511-1517, OUT. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café; Embrapa Unidades Centrais. |
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| 49. | | MOREIRA, R. V.; ROSA, S. D. V. F. da; MARQUES, K. K. M.; LACERDA FILHO, A. F. Sistema de secagem de café natural. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 42., 2016, Serra Negra, SP. Produzir mais café, com economia, só com boa tecnologia: trabalhos apresentados. Varginha: Fundação Procafé, 2016. 466 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 51. | | SAATH, R.; GIOMO, G. S.; BORÉM, F. M.; BIAGGIONI, M. A. M.; ROSA, S. D. V. F. da; BROETTO, F. Sensory quality alterations and activity of lea proteins during the coffee beans storage. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 137| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 56. | | SOUZA, L. N. C. de; CARDOSO, D. B.; OLIVEIRA, R. M. E.; ROSA, S. D. V. F. da; COELHO, E. V. B.; CLEMENTE, A. da C. S. Atributos físicos de grãos de café de diferentes níveis de qualidade da bebida. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA DA UFLA, 29., 2016, Lavras. [Anais...] Lavras: Universidade Federal de Lavras, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 57. | | GUIMARÃES, G. C.; ROSA, S. D. V. F. da; FREIRE, A. I.; CHAVES, A. R. C. da S.; PINTO, R. S. R. Avaliação da qualidade fisiológica de sementes de café por meio de imagens digitalizadas. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 58. | | GUIMARÃES, G. C.; ROSA, S. D. V. F. da; COELHO, L. F. S.; VEIGA, A. D.; CLEMENTE, A. da C. S. Avaliação do potencial germinativo de sementes de café em estádio precoce do desenvolvimento. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 59. | | RICALDONI, M. A.; ROSA, S. D. V. F. da; FIGUEIREDO, M. A. de; CARVALHO, M. A. de F.; FANTAZZINI, T. B. Avaliação de parâmetros de desenvolvimento de mudas de Coffea arabica L. originadas de sementes criopreservadas. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 10., 2019, Vitória, ES. Pesquisa, inovação e sustentabilidade dos cafés do Brasil. Brasília, DF: Embrapa Café, 2019. 4 p. Título em inglês: Evaluation of development parameters of Coffea arabica L. seedlings originated from criopreserved seeds.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 60. | | RICALDONI, M. A.; ROSA, S. D. V. F. da; CARVALHO, M. A. de F.; FIGUEIREDO, M. A. de; COELHO, S. V. B.; BAUTE, J. L.; FANTAZZINI, T. B. Avaliação de mudas de café originadas de sementes criopreservadas. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 45., 2019, Poços de Caldas. Mais tecnologias cafeeiras, nas cores brasileiras: trabalhos apresentados. Varginha: Fundação Procafé, 2019. p. 208-209 Apoio: Embrapa. CNPq, CAPES, FAPEMIG, INCT Café.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 157 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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