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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
02/03/2016 |
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Data da última atualização: |
09/05/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, F. C.; CLEMENTE, A. da C. S.; CAIXETA, F.; ROSA, S. D. V. F. da. |
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Afiliação: |
UDI Pesquisa & Desenvolvimento; UFLA; General Mills Brasil Alimentos Ltda.; STTELA DELLYZETE VEIGA F DA ROSA, SAPC. |
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Título: |
Endo-ß-mannanase and ß-tubulin gene expression during the final phases of coffee seed maturation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 4, p. 11719-11728, 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee seed maturation. MenosCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee se... Mostrar Tudo |
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Palavras-Chave: |
QRT-PCR. |
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Thesagro: |
Coffea Arábica. |
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Thesaurus Nal: |
Developmental stages. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/140475/1/Endo-B-mannanase.pdf
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Marc: |
LEADER 02122naa a2200193 a 4500
001 2039275
005 2019-05-09
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, F. C.
245 $aEndo-ß-mannanase and ß-tubulin gene expression during the final phases of coffee seed maturation.$h[electronic resource]
260 $c2015
520 $aCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the β-tubulin (TUB) and endo-β-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-β-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of β-tubulin during organogenesis and coffee seed maturation.
650 $aDevelopmental stages
650 $aCoffea Arábica
653 $aQRT-PCR
700 1 $aCLEMENTE, A. da C. S.
700 1 $aCAIXETA, F.
700 1 $aROSA, S. D. V. F. da
773 $tGenetics and Molecular Research$gv. 14, n. 4, p. 11719-11728, 2015.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 157 | |
| 22. |  | MATOS, D. P. de; ROSA, S. D. V. F. da; SANTOS, F. C.; SILVA, W. de S.; PEREIRA, C. C. Atividade da enzima-ß-manase em diferentes partes de sementes de café, antes e após a germinação. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 24. | | ROSA, S. D. V. F. da; CAIXETA, F.; CLEMENTE, A. da C. S.; PEREIRA, C. C.; SANTOS, F. C. Aspectos fisiológicos de grãos de café armazenados em ambiente resfriado. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 25. | | FIGUEIREDO, A. G.; ROSA, S. D. V. F. da; CLEMENTE, A. C. S.; MALTA, M. R.; VILELA, A. L. Antioxidant enzyme expression and its relationship to coffee beans quality. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 246.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 26. | | CLEMENTE, A. da C. S.; CIRILLO, M. A.; MALTA, M. R.; CAIXETA, F.; ROSA, S. D. V. F. da. Aplicações de técnicas de análise multivariada na avaliação da qualidade química e sensorial de café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 27. | | FERREIRA, I. A.; ROSA, S. D. V. F. da; McDONALD, M. B.; SCHWARTZ, S.; RIEDL, K.; VILELA, F. L. Capacidade antioxidante equivante ao trolox em sementes de Coffea arabica L. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 34., 2008, Caxambú. Anais... Brasília, DF: Embrapa Café, 2009.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 30. | | ROSA, S. D. V. F. da; FREITAS, M. N. de; SAÚDE, A.; PEREIRA, C. C.; CIRILLO, M. A. Imagens digitais do teste de tetrazólio para avaliar a qualidade de grãos de café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 8., 2013, Salvador. Sustentabilidade e inclusão Social. Brasília, DF: Embrapa Café, 2013.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 35. | | CARVALHO, A. M. de; ROSA, S. D. V. F. da; NOQUEIRA, F. D.; SANDY, E. C.; VEIGA, A. D. Formação de mudas de Coffea arábica L. a partir de sementes produzidas sob diferentes doses de fertilizantes nitrogenados. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 37. | | TAVEIRA, J. H. da S.; ROSA, S. D. V. F. da; BORÉM, F. M.; GIOMO, G. S.; SAATH, R. Perfis proteicos e desempenho fisiológico de sementes de café submetidas a diferentes métodos de processamento e secagem. PESQUISA AGROPECUÁRIA BRASILEIRA, Brasília, DF., V. 47, N. 10, P. 1511-1517, OUT. 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café; Embrapa Unidades Centrais. |
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| 38. | | GUIMARÃES, C. C.; ROSA, S. D. V. F. da; MALTA, M. R.; CARVALHO, M. H.; OLIVEIRA, R. M. E. Lipid content and fatty acid profile in Embryos, endosperm, and seeds of dried Coffea arabica. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 26., 2016, Kunming,Yunnan, China. Proceedings... ASIC - Association for Science and Information on Coffee, 2016. p. 120.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 157 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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