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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
04/01/2024 |
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Data da última atualização: |
04/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, F. C.; CLEMENTE, A. C. S.; CAIXETA, F.; ROSA, S. D. V. F. da. |
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Afiliação: |
F. C. SANTOS, UDI PESQUISA & DESENVOLVIMENTO; A. C. S. CLEMENTE, UNIVERSIDADE FEDERAL DE LAVRAS; F. CAIXETA, GENERAL MILLS BRASIL ALIMENTOS LTDA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa. |
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Título: |
Endo-β-mannanase and β-tubulin gene expression during the final phases of coffee seed maturation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 4 p. 11719-11728, 2015. |
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DOI: |
http://dx.doi.org/10.4238/2015.October.2.5 |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed maturation. MenosCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed matur... Mostrar Tudo |
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Thesaurus Nal: |
Coffea arabica var. arabica; Developmental stages; Organogenesis; Seed maturation. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/260321/1/Endo-mannanase.pdf
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Marc: |
LEADER 02224naa a2200217 a 4500
001 2160448
005 2024-01-04
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2015.October.2.5$2DOI
100 1 $aSANTOS, F. C.
245 $aEndo-β-mannanase and β-tubulin gene expression during the final phases of coffee seed maturation.$h[electronic resource]
260 $c2015
520 $aCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed maturation.
650 $aCoffea arabica var. arabica
650 $aDevelopmental stages
650 $aOrganogenesis
650 $aSeed maturation
700 1 $aCLEMENTE, A. C. S.
700 1 $aCAIXETA, F.
700 1 $aROSA, S. D. V. F. da
773 $tGenetics and Molecular Research$gv. 14, n. 4 p. 11719-11728, 2015.
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Registro original: |
Embrapa Café (CNPCa) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria de Alimentos. Para informações adicionais entre em contato com ctaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroindústria de Alimentos. |
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Data corrente: |
05/12/2018 |
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Data da última atualização: |
06/06/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CORRÊA, C. L. O.; LUNA, A. S.; PENHA, E. das M.; GOTTSCHALK, L. M. F. |
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Afiliação: |
Carla Levi O. Corrêa, UERJ; A. S. Luna, UERJ; EDMAR DAS MERCES PENHA, CTAA; LEDA MARIA FORTES GOTTSCHALK, CTAA. |
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Título: |
Determinação de acrilamida por espectrofluorêscencia em amostras de café. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PESQUISA S CAFEEIRAS, 44., 2018, Franca, SP. O Nosso café, melhorados desde o pé: Trabalhos apresentados. Varginha: Fundação Procafé, 2018. |
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Páginas: |
p. 182-183 |
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Idioma: |
Português |
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Notas: |
23 a 26 out. 2018. Seção 4.5. Colheita, preparo, qualidade do café, consumo e saúde. |
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Thesagro: |
Café; Produto de Origem Vegetal; Qualidade; Tecnologia de Alimento. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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Marc: |
LEADER 00820nam a2200205 a 4500
001 2100780
005 2023-06-06
008 2018 bl uuuu u01u1 u #d
100 1 $aCORRÊA, C. L. O.
245 $aDeterminação de acrilamida por espectrofluorêscencia em amostras de café.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE PESQUISA S CAFEEIRAS, 44., 2018, Franca, SP. O Nosso café, melhorados desde o pé: Trabalhos apresentados. Varginha: Fundação Procafé$c2018
300 $ap. 182-183
500 $a23 a 26 out. 2018. Seção 4.5. Colheita, preparo, qualidade do café, consumo e saúde.
650 $aCafé
650 $aProduto de Origem Vegetal
650 $aQualidade
650 $aTecnologia de Alimento
700 1 $aLUNA, A. S.
700 1 $aPENHA, E. das M.
700 1 $aGOTTSCHALK, L. M. F.
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Embrapa Agroindústria de Alimentos (CTAA) |
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