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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
28/12/2010 |
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Data da última atualização: |
20/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SEIBERT, C. H.; BORSA, M.; ROSA, R. D.; CARGNIN-FERREIRA, E.; PEREIRA, A. M. L.; GRISARD, E. C.; ZANETTI, C. R.; PINTO, A. R. |
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Afiliação: |
CAROLINE H. SEIBERT, UNIVERSIDADE FEDERAL DE SANTA CATARINA; MARIANA BORSA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; RAFAEL D. ROSA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; EDUARDO CARGNIN-FERREIRA, UNIVERSIDADE FEDERAL DE SANTA CATARINA; ALITIENE MOURA LEMOS PEREIRA, CPAMN; EDMUNDO C. GRISARD, UNIVERSIDADE FEDERAL DE SANTA CATARINA; CARLOS R. ZANETTI, UNIVERSIDADE FEDERAL DE SANTA CATARINA; AGUINALDO R. PINTO, UNIVERSIDADE FEDERAL DE SANTA CATARINA. |
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Título: |
Detection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Virological Methods, Amsterdam-Holanda, v. 169, n. 1, p. 169-175, 2010. |
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Idioma: |
Inglês |
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Conteúdo: |
Infectious myonecrosis virus (IMNV) has been causing a progressive disease in farm-reared shrimps in Brazil and Indonesia. Immunodiagnosticmethods for IMNVdetection, although reliable, are not employed currently becausemonoclonal antibodies (MAbs) against this virus are not available. In this study, a fragment of the IMNVmajor capsid protein gene, comprising amino acids 300?527 (IMNV300?527),was cloned and expressed in Escherichia coli. The nucleotide sequence of the recombinant IMNV300?527 fragment displayed a high degree of identity to the major capsid protein of IMNV isolates from Brazil (99%) and Indonesia (98%). Ten MAbs were generated against the expressed fragment, and eight of these, mostly IgG2a or IgG2b,were able to bind to IMNVin tissue extracts fromshrimps infected naturally in immunodotblot assays. Six of these MAbs recognized a ?100 kDa protein in a Western-blot, which is the predicted mass of IMNV major capsid protein, and also bound to viral inclusions present in muscle ?broses and in coagulativemyonecrosis, as demonstrated by immunohistochemistry. Among all thoseMAbs created, four did not cross-react with non-infected shrimp tissues; this observation supports their applicability as a sensitive and speci?c immunodiagnosis of IMNV infection in shrimps. |
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Palavras-Chave: |
Proteína recombinante; Vírus da mionecrose infecciosa. |
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Thesagro: |
Anticorpo Monoclonal; Camarão. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/24511/1/JOURNAL.pdf
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Marc: |
LEADER 02064naa a2200253 a 4500
001 1870999
005 2022-06-20
008 2010 bl uuuu u00u1 u #d
100 1 $aSEIBERT, C. H.
245 $aDetection of major capsid protein of infectious myonecrosis virus in shrimps using monoclonal antibodies.
260 $c2010
520 $aInfectious myonecrosis virus (IMNV) has been causing a progressive disease in farm-reared shrimps in Brazil and Indonesia. Immunodiagnosticmethods for IMNVdetection, although reliable, are not employed currently becausemonoclonal antibodies (MAbs) against this virus are not available. In this study, a fragment of the IMNVmajor capsid protein gene, comprising amino acids 300?527 (IMNV300?527),was cloned and expressed in Escherichia coli. The nucleotide sequence of the recombinant IMNV300?527 fragment displayed a high degree of identity to the major capsid protein of IMNV isolates from Brazil (99%) and Indonesia (98%). Ten MAbs were generated against the expressed fragment, and eight of these, mostly IgG2a or IgG2b,were able to bind to IMNVin tissue extracts fromshrimps infected naturally in immunodotblot assays. Six of these MAbs recognized a ?100 kDa protein in a Western-blot, which is the predicted mass of IMNV major capsid protein, and also bound to viral inclusions present in muscle ?broses and in coagulativemyonecrosis, as demonstrated by immunohistochemistry. Among all thoseMAbs created, four did not cross-react with non-infected shrimp tissues; this observation supports their applicability as a sensitive and speci?c immunodiagnosis of IMNV infection in shrimps.
650 $aAnticorpo Monoclonal
650 $aCamarão
653 $aProteína recombinante
653 $aVírus da mionecrose infecciosa
700 1 $aBORSA, M.
700 1 $aROSA, R. D.
700 1 $aCARGNIN-FERREIRA, E.
700 1 $aPEREIRA, A. M. L.
700 1 $aGRISARD, E. C.
700 1 $aZANETTI, C. R.
700 1 $aPINTO, A. R.
773 $tJournal of Virological Methods, Amsterdam-Holanda$gv. 169, n. 1, p. 169-175, 2010.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/04/2019 |
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Data da última atualização: |
06/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SÁ, J. F. de; SAMPAIO, E. dos S.; MENDES, M. I. de S.; SANTOS, K. C. F. dos; SOUZA, A. da S.; LEDO, C. A. da S. |
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Afiliação: |
JUCIENY FERREIRA DE SÁ, UFRB; UFRB; MARIA INÊS DE SOUZA MENDES, UESC; KAREN CRISTINA FIALHO DOS SANTOS, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Culture media for the multiplication of wild Manihot species meios de cultura para a multiplicação de espécies silvestres de Manihot. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Ciência e Agrotecnologia, v.42, n.6, p.598-607, Nov./Dec. 2018 |
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ISSN: |
1981-1829 |
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Idioma: |
Inglês |
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Conteúdo: |
The cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot. MenosThe cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species... Mostrar Tudo |
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Thesagro: |
Cultura de Tecido; Mandioca; Micropropagação. |
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Thesaurus NAL: |
Micropropagation; Tissue culture. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02332naa a2200253 a 4500
001 2107902
005 2019-12-06
008 2018 bl uuuu u00u1 u #d
022 $a1981-1829
100 1 $aSÁ, J. F. de
245 $aCulture media for the multiplication of wild Manihot species meios de cultura para a multiplicação de espécies silvestres de Manihot.$h[electronic resource]
260 $c2018
520 $aThe cassava propagation system is slow and favors disease transmission through successive generations. Micropropagation is an alternative to overcome the aforementioned limitations, besides allowing the generation of a larger number of pest- and pathogen-free plants. Therefore, the aim of the present study is to investigate the effect of culture media on the multiplication in vitro of five wild Manihot species. The experiment followed a completely randomized design, at factorial arrangement 5 (wild Manihot species) x 6 (culture media), with 11 repetitions. Explants consisted in nodal segments (91 cm long and one lateral bud) of species Manihot flabellifolia, M. tristis, M. caerulescens, M. chlorosticta and M. jacobinensis, which were extracted in vitro from the collection of wild cassava species. One segment was placed in each test tube added with 10 mL of MS media 0.01, 17N, 12A3, 4E, 8S and WPM, and kept for 90 days in growth room under 30 ?mol m-2 s-1irradiance, temperature 27 ± 1 °C and 16h photoperiod. Variables plant height (cm), number of green leaves, number of senescent leaves, number of shoots, number of microcuttings, fresh and dry shoot mass, fresh and dry root mass (mg) and callus mass (mg) were analyzed. Our results showed that the culture medium 12A3 was not responsive to any of the species; however, if one takes into consideration variables plant height and number of microcuttings, this medium can possibly be used in the micropropagation of other wild species belonging to genus Manihot.
650 $aMicropropagation
650 $aTissue culture
650 $aCultura de Tecido
650 $aMandioca
650 $aMicropropagação
700 1 $aSAMPAIO, E. dos S.
700 1 $aMENDES, M. I. de S.
700 1 $aSANTOS, K. C. F. dos
700 1 $aSOUZA, A. da S.
700 1 $aLEDO, C. A. da S.
773 $tCiência e Agrotecnologia$gv.42, n.6, p.598-607, Nov./Dec. 2018
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Embrapa Mandioca e Fruticultura (CNPMF) |
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