Data/hora: 01/06/2024 03:43:33

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 21/09/1993
Data da última atualização: | 21/09/1993
Autoria: | ALBANESE, G.; RENIS, M.; GRIMALDI, V.; LA ROSA, R.; POLIZZI, G.; DIENER, T. O.
Afiliação: | Istituto de Patologia Vegetable, Universita, 95123 Catania, Italy.
Título: | Hybridization analysis of citrus viroids with citrus exocortis viroid-and hop stunt viroid-specific probes.
Ano de publicação: | 1991
Fonte/Imprenta: | Riverside, California: International Organization of Citrus Virologists, 1991.
Páginas: | p.202-205
Idioma: | Inglês
Notas: | Conference of the International Organization of Citrus Virologists, 11., 1991, Riverside, California, Proceedings...
Conteúdo: | Nucleic acids extracted from viroid-infected commercial citrus trees were analyzed by bidirectional electrophoresis in 7% polyacrylamide gels with the second-direction run performed under denaturing conditions either at homogeneous pH (8.3) or at discontinous pH (8.3-6.5). Electrotransfer of gel samples to nylon menbranes, followed by molecular hybridization tests with citrus exocortis viroids (CEVd)- or hop stunt viroid (HSVd)- specific nucleic acid probes revealed that, of all the viroids detected, only CEVd reacted with the CEVd-specific probes, whereas two viroids reacted with the HSVd-specific probes. Identity of these viroids is discussed in comparison with results of other investigators.
Palavras-Chave: | Electroforese.
Thesagro: | Doença; Laranja.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (13296-1) - ADC - PL - -- - 634.3040601 - C748p - 1992000183 - DIS