Data/hora: 08/06/2024 04:47:41

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 28/12/2023
Data da última atualização: | 22/01/2024
Autoria: | ROSA, P. M. da S.; GUEDES, P. H. E.; GARCIA, J. M.; OLIVEIRA, C. S.
Afiliação: | PAOLA MARIA DA SILVA ROSA, UNIVERSIDADE ESTADUAL PAULISTA; PEDRO HENRIQUE EVAGELISTA GUEDES; JOAQUIM MANSANO GARCIA, UNIVERSIDADE ESTADUAL PAULISTA; CLARA SLADE OLIVEIRA, CNPGL.
Título: | Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development.
Ano de publicação: | 2023
Fonte/Imprenta: | Zygote, v. 32, n. 1, p. 28-37, 2023.
DOI: | https://doi.org/10.1017/S0967199423000576
Idioma: | Inglês
Conteúdo: | Oocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence.
Palavras-Chave: | Classificação morfológica; IVF; Oócito; Ooplasma; Ovócito.
Thesagro: | Bovino; Citoplasma; Embrião Animal; Granulo.
Thesaurus Nal: | Cytoplasm; Granules.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (26230-1) - UPC - AP - DD -  -  -  - DIS