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Registro Completo |
Biblioteca(s): |
Embrapa Agroindústria de Alimentos; Embrapa Cerrados. |
Data corrente: |
20/09/2022 |
Data da última atualização: |
08/12/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
STEPHAN, M. P.; COSTA, A. M.; AZEVEDO, T. de L.; ROSA, J. S. da; SANTOS, A. A. dos. |
Afiliação: |
MARILIA PENTEADO STEPHAN, CTAA; ANA MARIA COSTA, CPAC; TATIANA DE LIMA AZEVEDO, CTAA; JEANE SANTOS DA ROSA, CTAA; ALEXSANDRO ARAUJO DOS SANTOS, CTAA. |
Título: |
Electrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Journal of Agricultural Science and Technology B, v. 12, n. 2, p. 40-47, 2022. |
DOI: |
https://doi.org/10.17265/2161-6264/2022.02.002 |
Idioma: |
Inglês |
Conteúdo: |
Brazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them. MenosBrazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as ... Mostrar Tudo |
Palavras-Chave: |
Bioactive peptides; Mass distribution on fruit; P setacea; P tenuifila. |
Thesagro: |
Casca; Eletroforese; Fruto; Maracujá; Método de Análise; Peptídeo; Polpa; Proteína; Purificação; Rastreamento; Semente; Tecnologia de Alimento. |
Thesaurus Nal: |
Analytical methods; Bioactive compounds; Food technology; Fruits; Passiflora; Passion fruits; Peptides; Polyacrylamide gel electrophoresis; Proteins; Pulp; Purification methods; Seeds. |
Categoria do assunto: |
Q Alimentos e Nutrição Humana |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146660/1/62ea112170472.pdf
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Marc: |
LEADER 03088naa a2200517 a 4500 001 2146660 005 2022-12-08 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.17265/2161-6264/2022.02.002$2DOI 100 1 $aSTEPHAN, M. P. 245 $aElectrophoresis (SDS-PAGE) as a method for screening species of Passiflora using seed proteins as molecular markers.$h[electronic resource] 260 $c2022 520 $aBrazil is the center of diversity and dispersion of species of the genus Passiflora. Two species of passion fruit Passiflora tenuifila BRS VT (passiflora garlic) and another of Passiflora setacea BRS PC were used in this study. The plants were grown on the Embrapa Cerrados experimental farm. The fruits were washed, minimally cut and their constituents separated. Both species differed in the proportion of wet mass between husk, seed, and pulp. As expected, both species had a higher proportion of husks and seeds and a lower amount of pulp. A semi-purification procedure for the proteins present in the seeds and husks was described and the molecular aspects were analyzed by polyacrylamide gel electrophoresis (SDS-PAGE). The quantification of soluble proteins was performed using the Bradford method. The analysis of the soluble protein extracted from the seeds and husks showed that these values are up to 70 times higher for the seeds of P. tenuifila and 28.5 times for P. setacea, in the husks. It was verified that both species present a similar protein profile, observed by the intense and diverse bands found in the polyacrylamide gel, mainly in the range of 32 to 19 kDa. This work opens up an unexplored field of tracking bioactive proteins and/or peptides including forms of nanostructure systems that protect other bioactive molecules. The objective of this work was to present an analytical procedure to semi-purify seed proteins of two Passiflora species and use electrophoresis as an analysis tool for further screening of their protein profiles and selection of a molecular marker to differentiate them. 650 $aAnalytical methods 650 $aBioactive compounds 650 $aFood technology 650 $aFruits 650 $aPassiflora 650 $aPassion fruits 650 $aPeptides 650 $aPolyacrylamide gel electrophoresis 650 $aProteins 650 $aPulp 650 $aPurification methods 650 $aSeeds 650 $aCasca 650 $aEletroforese 650 $aFruto 650 $aMaracujá 650 $aMétodo de Análise 650 $aPeptídeo 650 $aPolpa 650 $aProteína 650 $aPurificação 650 $aRastreamento 650 $aSemente 650 $aTecnologia de Alimento 653 $aBioactive peptides 653 $aMass distribution on fruit 653 $aP setacea 653 $aP tenuifila 700 1 $aCOSTA, A. M. 700 1 $aAZEVEDO, T. de L. 700 1 $aROSA, J. S. da 700 1 $aSANTOS, A. A. dos 773 $tJournal of Agricultural Science and Technology B$gv. 12, n. 2, p. 40-47, 2022.
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Registro original: |
Embrapa Agroindústria de Alimentos (CTAA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
09/09/2014 |
Data da última atualização: |
23/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
SOUZA, T. C. de; MAGALHAES, P. C.; CASTRO, E. M. de; CARNEIRO, N. P.; PADILHA, F. A.; GOMES JÚNIOR, C. C. |
Afiliação: |
PAULO CESAR MAGALHAES, CNPMS; NEWTON PORTILHO CARNEIRO, CNPMS. |
Título: |
ABA application to maize hybrids contrasting for drought tolerance: changes in water parameters and in antioxidant enzyme activity. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plant Growth Regulation, v. 73, p. 205-217, 2014. |
DOI: |
10.1007/s10725-013-9881-9 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to evaluate the effects of abscisic acid (ABA) related to the increase of water-stress tolerance in two drought contrasting maize hybrids: DKB 390 (tolerant) and BRS 1030 (sensitive). Thecharacterization of water status (pre-dawn leaf water potential, Wpd; midday leaf water potential, Wmdand stem water potential, Wst) and antioxidant enzyme activity was conducted on greenhouse grown plants. The ABA, hydrogen peroxide (H2O2), and malondialdehyde (MDA) contents were also analyzed. Water deficit was imposed for 10 days at the flowering stage and a dosage of 100lM ABA was applied to plant canopy. Measurements were taken during 10 days after the water recovery. With 5 days of stress, the tolerant hybrid showed lower MDA content, decrease in the water status, and higher activity of the enzymes superoxide dismutase, catalase, ascorbate peroxidase, as well as guaiacol, glutathione reductase, dehydroascorbate reductase, polyphenol oxidase, and L-phenylalanine ammonia-lyase, as compared to the sensitive hybrid. With 10 days of stress, DKB 390 had a decrease in the activity of enzymes whereas BRS 1030 showed a higher activity. In addition, the lattershowed greater amounts of H2O2 and MDA. ABA application led to a higher tolerance only in DKB 390, due to the increase of water status and the enzymatic activity, mainly the catalase. |
Palavras-Chave: |
Estresse hídrico; Estresse oxidativo. |
Thesagro: |
Zea mays. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02108naa a2200229 a 4500 001 1994537 005 2017-05-23 008 2014 bl uuuu u00u1 u #d 024 7 $a10.1007/s10725-013-9881-9$2DOI 100 1 $aSOUZA, T. C. de 245 $aABA application to maize hybrids contrasting for drought tolerance$bchanges in water parameters and in antioxidant enzyme activity.$h[electronic resource] 260 $c2014 520 $aThe objective of this study was to evaluate the effects of abscisic acid (ABA) related to the increase of water-stress tolerance in two drought contrasting maize hybrids: DKB 390 (tolerant) and BRS 1030 (sensitive). Thecharacterization of water status (pre-dawn leaf water potential, Wpd; midday leaf water potential, Wmdand stem water potential, Wst) and antioxidant enzyme activity was conducted on greenhouse grown plants. The ABA, hydrogen peroxide (H2O2), and malondialdehyde (MDA) contents were also analyzed. Water deficit was imposed for 10 days at the flowering stage and a dosage of 100lM ABA was applied to plant canopy. Measurements were taken during 10 days after the water recovery. With 5 days of stress, the tolerant hybrid showed lower MDA content, decrease in the water status, and higher activity of the enzymes superoxide dismutase, catalase, ascorbate peroxidase, as well as guaiacol, glutathione reductase, dehydroascorbate reductase, polyphenol oxidase, and L-phenylalanine ammonia-lyase, as compared to the sensitive hybrid. With 10 days of stress, DKB 390 had a decrease in the activity of enzymes whereas BRS 1030 showed a higher activity. In addition, the lattershowed greater amounts of H2O2 and MDA. ABA application led to a higher tolerance only in DKB 390, due to the increase of water status and the enzymatic activity, mainly the catalase. 650 $aZea mays 653 $aEstresse hídrico 653 $aEstresse oxidativo 700 1 $aMAGALHAES, P. C. 700 1 $aCASTRO, E. M. de 700 1 $aCARNEIRO, N. P. 700 1 $aPADILHA, F. A. 700 1 $aGOMES JÚNIOR, C. C. 773 $tPlant Growth Regulation$gv. 73, p. 205-217, 2014.
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