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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
02/03/2021 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. |
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Afiliação: |
A. F. Cunha, UFMG; H. M. Andrade, UFMG; F. N. Souza, UFMG; L. C. Fialho Júnior, UFMG; D. L. S. O. Rosa, UFMG; E. M. Ramos Sanchez, USP; M. Gidlund, USP; H. Goto, USP; M. A. V. P. Brito, Pesquisadora aposentada da Embrapa Gado de Leite.; ALESSANDRO DE SA GUIMARAES, CNPGL; A. P. Lage, UFMG; L. C. Reis, USP; A. M. M. P. Della Libera, USP; M. B. Heinemann, USP; M. M. O. P. Cerqueira, UFMG. |
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Título: |
Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets. MenosABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among ani... Mostrar Tudo |
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Palavras-Chave: |
Immunoproteomics; Intramammary infection; Serological proteome analysis. |
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Thesagro: |
Staphylococcus Aureus. |
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Thesaurus Nal: |
Vaccines. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02811naa a2200349 a 4500
001 2130440
005 2024-02-06
008 2020 bl uuuu u00u1 u #d
100 1 $aCUNHA, A. F.
245 $aComparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine.$h[electronic resource]
260 $c2020
520 $aABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cowto-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus, further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F0F1 ATP synthase subunit ?, succinyl-diaminopimelate desuccinylase, and cysteinyl- tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.
650 $aVaccines
650 $aStaphylococcus Aureus
653 $aImmunoproteomics
653 $aIntramammary infection
653 $aSerological proteome analysis
700 1 $aANDRADE, H. M.
700 1 $aSOUZA, F. N.
700 1 $aFIALHO JUNIOR, L. C.
700 1 $aROSA, D. L. S. O.
700 1 $aSANCHEZ, E. M. R.
700 1 $aGIDLUND, M.
700 1 $aGOTO, H.
700 1 $aBRITO, M. A. V. P.
700 1 $aGUIMARÃES, A. S.
700 1 $aLAGE, A. P.
700 1 $aREIS, L. C.
700 1 $aLIBERA, A. M. M. P. D.
700 1 $aHEINEMANN, M. B.
700 1 $aCERQUEIRA, M. M. O. P
773 $tJournal of Dairy Science$gv. 103, n. 5, p. 4588-4605, 2020.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
10/02/2014 |
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Data da última atualização: |
10/02/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
REIMANN, F. A.; AZAMBUJA, R. C. de C.; RODRIGUES, P. F.; BALDISSERA, J.; FEIJÓ, J. de O.; MENDONÇA, F. de S.; COSTA, R. F.; FREITAS, S. F.; SCHNEIDER, A.; CARDOSO, F. F. |
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Afiliação: |
FERNANDO ANTONIO REIMANN, UNIJUÍ; RODRIGO CARNEIRO DE CAMPOS AZAMBUJA, DOUTORANDO UFPEL; PEDRO FARACO RODRIGUES, SENAR-RS; JAQUELINE BALDISSERA, UNICRUZ; JOSIANE DE OLIVEIRA FEIJÓ, DOUTORANDA UFPEL; FABIO DE SOUZA MENDONÇA, MESTRANDO UFPEL; RODRIGO FAGUNDES COSTA, MESTRANDO UFPEL; SILVIA FREITAS FREITAS, GRADUANDA UFPEL; AUGUSTO SCHNEIDER, UFPEL; FERNANDO FLORES CARDOSO, CPPSUL. |
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Título: |
Concentrações séricas de insulina no pós-parto recente de vacas de corte de diferentes genótipos criadas no Sul do Brasil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: SEMINÁRIO INTERINSTITUCIONAL DE ENSINO, PESQUISA E EXTENSÃO, 18.; MOSTRA DE INICIAÇÃO CIENTÍFICA, 16,; MOSTRA DE EXTENSÃO, 11., 2013, Cruz Alta. Ciência, conhecimento e sociedade de risco: anais. Cruz Alta: Unicruz, 2013. |
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Idioma: |
Português |
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Thesagro: |
Bovino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96828/1/Cardoso-cctrcao-insulina.pdf
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Marc: |
LEADER 00883nam a2200217 a 4500
001 1979246
005 2014-02-10
008 2013 bl uuuu u00u1 u #d
100 1 $aREIMANN, F. A.
245 $aConcentrações séricas de insulina no pós-parto recente de vacas de corte de diferentes genótipos criadas no Sul do Brasil.$h[electronic resource]
260 $aIn: SEMINÁRIO INTERINSTITUCIONAL DE ENSINO, PESQUISA E EXTENSÃO, 18.; MOSTRA DE INICIAÇÃO CIENTÍFICA, 16,; MOSTRA DE EXTENSÃO, 11., 2013, Cruz Alta. Ciência, conhecimento e sociedade de risco: anais. Cruz Alta: Unicruz$c2013
650 $aBovino
700 1 $aAZAMBUJA, R. C. de C.
700 1 $aRODRIGUES, P. F.
700 1 $aBALDISSERA, J.
700 1 $aFEIJÓ, J. de O.
700 1 $aMENDONÇA, F. de S.
700 1 $aCOSTA, R. F.
700 1 $aFREITAS, S. F.
700 1 $aSCHNEIDER, A.
700 1 $aCARDOSO, F. F.
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Embrapa Pecuária Sul (CPPSUL) |
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