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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
26/05/1998 |
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Data da última atualização: |
07/05/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SCRAMIN, S.; ROQUE, M. R. A.; DENTZIEN, A. F. M.; ROSSO, C. R. S. de; MELO, I. S. de. |
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Afiliação: |
SHIRLEI SCRAMIN, EMBRAPA-CNPMA; ANAMARIA FERREIRA MAYER DENTZIEN, CNPMA; CESAR ROBERTO SILVA DE ROSSO, CNPMA; ITAMAR SOARES DE MELO, CNPMA. |
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Título: |
Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: LATIN AMERICAN BIODEGRADATION & BIODETERIORATION SYMPOSIUM, 3., 1998, Florianopolis. Resumos. Florianopolis: UFSC, 1998. |
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Páginas: |
p.16 |
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Idioma: |
Inglês |
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Conteúdo: |
Two Acinetobacter spp. strains, denominated D-12 and D-16 (extracted from Diuron TM treated sugarcane rhizosphere) were grown in different substrates in order to determine whether catechol 1,2-dioxygenase (pyrocatechase) and catechol 2,3-dioxygenase (metapyrocatechase) are constitutive or induced enzymes. Extracellular enzymes were also measured. Although biomass was measured, the conditions required for best production of microorganisms were not established. Five different inducers were added to culture media: glucose, sodium benzoate, Diuron TM, dichloroaniline and Diuron TM plus glucose. Both strains were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. Extracellular enzyme assays were carried out with filtered supernatant. Both strains showed significant catechol 1,2-dioxygenase activity when sodium benzoate was added, but not with other inducers. Significant manganese (MnP) and lignin (LiP) peroxidase activity was observed in all supernatants, except for D-16 in which LiP was not detected with sodium benzoate. Data obtained showed evidence of the inductive nature of catechol 1,2-dioxygenase. |
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Palavras-Chave: |
Acinetobacter spp; Extracellular enzymes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197060/1/1998RA-Scramin-Presence.pdf
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Marc: |
LEADER 01838nam a2200193 a 4500
001 1012899
005 2019-05-07
008 1998 bl uuuu u00u1 u #d
100 1 $aSCRAMIN, S.
245 $aPresence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers.$h[electronic resource]
260 $aIn: LATIN AMERICAN BIODEGRADATION & BIODETERIORATION SYMPOSIUM, 3., 1998, Florianopolis. Resumos. Florianopolis: UFSC$c1998
300 $ap.16
520 $aTwo Acinetobacter spp. strains, denominated D-12 and D-16 (extracted from Diuron TM treated sugarcane rhizosphere) were grown in different substrates in order to determine whether catechol 1,2-dioxygenase (pyrocatechase) and catechol 2,3-dioxygenase (metapyrocatechase) are constitutive or induced enzymes. Extracellular enzymes were also measured. Although biomass was measured, the conditions required for best production of microorganisms were not established. Five different inducers were added to culture media: glucose, sodium benzoate, Diuron TM, dichloroaniline and Diuron TM plus glucose. Both strains were harvested by centrifugation and disrupted by sonication for intracellular enzyme extraction. Extracellular enzyme assays were carried out with filtered supernatant. Both strains showed significant catechol 1,2-dioxygenase activity when sodium benzoate was added, but not with other inducers. Significant manganese (MnP) and lignin (LiP) peroxidase activity was observed in all supernatants, except for D-16 in which LiP was not detected with sodium benzoate. Data obtained showed evidence of the inductive nature of catechol 1,2-dioxygenase.
653 $aAcinetobacter spp
653 $aExtracellular enzymes
700 1 $aROQUE, M. R. A.
700 1 $aDENTZIEN, A. F. M.
700 1 $aROSSO, C. R. S. de
700 1 $aMELO, I. S. de
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Embrapa Meio Ambiente (CNPMA) |
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| Registros recuperados : 7 | |
| 5. |  | ROQUE, M. R. A.; MELO, I. S. de; BARBIERI, S. M.; MORYAMA, A. S.; NAKAYAMA, C. R. Distribuição de DNA plasmidial em linhagens resistentes ao herbicida diuron. In: ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 5.; ENCONTRO NORDESTINO DE MICROBIOLOGIA AMBIENTAL, 1., 1996, Fortaleza. Livro de resumos. Fortaleza: Universidade Federal do Ceara, 1996. p.108| Biblioteca(s): Embrapa Meio Ambiente. |
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| 7. | | SCRAMIN, S.; ROQUE, M. R. A.; DENTZIEN, A. F. M.; ROSSO, C. R. S. de; MELO, I. S. de. Presence of intra and extracellular enzymes in strains of Acinetobacter spp, produced in different inducers. In: LATIN AMERICAN BIODEGRADATION & BIODETERIORATION SYMPOSIUM, 3., 1998, Florianopolis. Resumos. Florianopolis: UFSC, 1998. p.16| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| Registros recuperados : 7 | |
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