Data/hora: 03/06/2024 17:53:53

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	06/07/2009	
Data da última atualização: 	22/08/2022	
Autoria: 	DIE, J. V.; ROMAN, B.; SALVADOR, N.; RODRIGUEZ, M. A. D.; GONZÁLEZ-VERDEJO, C. I.	
Afiliação: 	José Vicente Die, IFAPA; Belén Román, IFAPA; Nadal Moyano Salvador, IFAPA; MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; Clara Isabel González-Verdejo, IFAPA.	
Título: 	Expression analysis of Pisum sativum putative defence genes during Orobanche crenata infection.	
Ano de publicação: 	2009	
Fonte/Imprenta: 	Crop and Pasture Science, Victoria, v. 60, n. 5, p. 490-498, mai. 2009.	
ISSN: 	1836-0947	
DOI: 	DOI: 10.1071/CP08274	
Idioma: 	Inglês	
Conteúdo: 	The root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental issue for the genetic improvement of legumes. In this work, the temporal expression patterns of 8 defence-genes known to be involved in different metabolic pathways activated during several plant-pathogen interactions were investigated in Pisum sativum. Molecular analyses were carried out using quantitative real-time polymerase chain reaction during the initial stages of the parasitisation process in susceptible (Messire) and incompletely resistant (Ps624) pea genotypes. Transcriptional changes in response to O. crenata revealed induction of genes putatively encoding pathogenesis-related proteins, peroxidase activity, and dehydration stress-responsive signalling. This, combined with high constitutive gene expression mediating the phenylpropanoid pathway were observed as part of the defence mechanisms triggered in Ps624 to restrict the growth of the parasite.	
Palavras-Chave: 	Plant defence; Real-time PCR.	
Thesagro: 	Melhoramento Genético Vegetal; Patógeno.	
Thesaurus Nal: 	parasitic plants.	
Categoria do assunto: 	G Melhoramento Genético	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/655752/1/Expression-analysis-of-Pisum-sativum-putative-defence-genes-during-Orobanche-crenata-infect.pdf	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (25696-1) - UPC - AP - DD -  -  - 12784 - DIS