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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/10/1992 |
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Data da última atualização: |
21/10/1992 |
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Autoria: |
SADOWSKY, M. J.; CREGAN, P. B.; GOTTFERT, M.; SHARMA, A.; GERHOLD, D.; RODRIGUEZ-QUINONES, F.; KEYSER, H. H.; KENNECKE, H.; STACEY, G. |
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Afiliação: |
Soil Science Department, University of Minnesota, 246 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN 55108. |
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Título: |
The Bradyrhizobium japonicum nolA gene and its involvement in the genotype-specific nodulation of soybeans. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Proceedings of the National Academy of Science, v.88, p.637-641, 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
Several soybean genotypes have been identified which specifically exclude nodulation by members of Bradyrhizobium japonicum serocluster 123. We have identified and sequenced a DNA region from B. japonicum strain USDA 110 which is involved in genotype-specific nodulation of soybeans. This 2.3-kilobase region, cloned in pMJS12, allows B. japonicum serocluster 123 isolates to form nodules on plants of serogroup 123-restricting genotypes. The nodules, however, were ineffective for symbiotic nitrogen fixation. The nodulation-complementing region is located approximately 590 base pairs transcriptionally downstream from nodD2. The 5' end of pMJS12 contains a putative open reading frame (ORF) of 710 base pairs, termed nolA. Transposon Tn3-HoHo mutations only within the ORF abolished nodulation complementation. The N terminus of the predicted nolA gene product has strong similarity with the N terminus of MerR, the regulator of mercury resistance genes. Translational lacZ fusion experiments indicated that nolA was moderately induced by soybean seed stract and the isoflavone genistein. Restriction fragments that hybridize to pMJS12 were detected in genomic DNAs from both nodulation-restricted and -unrestricted strains. |
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Palavras-Chave: |
B; Fixacao; Fixation; Genetic; Isoflavonoide; japonicum; N2; Nod; Soybean; Transcicao; Transcription; Transposon. |
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Thesagro: |
Flavonóide; Genética; Nodulação; Soja. |
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Thesaurus Nal: |
flavonoids; genes; isoflavonoids; nodulation. |
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Categoria do assunto: |
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Marc: |
LEADER 02372naa a2200457 a 4500
001 1458153
005 1992-10-21
008 1991 bl --- 0-- u #d
100 1 $aSADOWSKY, M. J.
245 $aThe Bradyrhizobium japonicum nolA gene and its involvement in the genotype-specific nodulation of soybeans.
260 $c1991
520 $aSeveral soybean genotypes have been identified which specifically exclude nodulation by members of Bradyrhizobium japonicum serocluster 123. We have identified and sequenced a DNA region from B. japonicum strain USDA 110 which is involved in genotype-specific nodulation of soybeans. This 2.3-kilobase region, cloned in pMJS12, allows B. japonicum serocluster 123 isolates to form nodules on plants of serogroup 123-restricting genotypes. The nodules, however, were ineffective for symbiotic nitrogen fixation. The nodulation-complementing region is located approximately 590 base pairs transcriptionally downstream from nodD2. The 5' end of pMJS12 contains a putative open reading frame (ORF) of 710 base pairs, termed nolA. Transposon Tn3-HoHo mutations only within the ORF abolished nodulation complementation. The N terminus of the predicted nolA gene product has strong similarity with the N terminus of MerR, the regulator of mercury resistance genes. Translational lacZ fusion experiments indicated that nolA was moderately induced by soybean seed stract and the isoflavone genistein. Restriction fragments that hybridize to pMJS12 were detected in genomic DNAs from both nodulation-restricted and -unrestricted strains.
650 $aflavonoids
650 $agenes
650 $aisoflavonoids
650 $anodulation
650 $aFlavonóide
650 $aGenética
650 $aNodulação
650 $aSoja
653 $aB
653 $aFixacao
653 $aFixation
653 $aGenetic
653 $aIsoflavonoide
653 $ajaponicum
653 $aN2
653 $aNod
653 $aSoybean
653 $aTranscicao
653 $aTranscription
653 $aTransposon
700 1 $aCREGAN, P. B.
700 1 $aGOTTFERT, M.
700 1 $aSHARMA, A.
700 1 $aGERHOLD, D.
700 1 $aRODRIGUEZ-QUINONES, F.
700 1 $aKEYSER, H. H.
700 1 $aKENNECKE, H.
700 1 $aSTACEY, G.
773 $tProceedings of the National Academy of Science$gv.88, p.637-641, 1991.
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Registro original: |
Embrapa Soja (CNPSO) |
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