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Registro Completo |
Biblioteca(s): |
Embrapa Meio Norte / UEP-Parnaíba. |
Data corrente: |
13/05/1996 |
Data da última atualização: |
13/05/1996 |
Autoria: |
VILLALOBOS-RODRIGUEZ, E.; SHIBLES, R. |
Afiliação: |
Universidad de Costa Rica. |
Título: |
Response of determinate and indeterminate tropical soybean cultivars to water stress. |
Ano de publicação: |
1985 |
Fonte/Imprenta: |
Field Crops Research, v.10, p.269-281, 1985. |
Idioma: |
Inglês |
Conteúdo: |
Field and glasshouse experiments were conducted at the University of Costa Rica experiment station to test the hypothesis that indeterminatetropical soybean (Glycine max L.Merr) cultivars could better tolerate moisture stress than determinate types. In the field (dry season 1982)stress treatments, consisting of withholding water for 15 days, were given before flowering and during the R2 (full bloom) and the R3 (early podding) stages of plant development. In the glasshouse (August-nobember, 1982) stress treatments, consisting of withholding water for 10 days, were imposed preflowering,R2, R4 (mid-podding) and R6 (mid-seedfilling). Field-grown plants under stress had predawn leaf water potentials that were 0,1 - 0,2 MPa less than those of well-watered control plants 7 days after withholding water began. Stomata showed the most rapid decline in water vapor conductance at leaf water potential between -1,2 and - 1,4 MPa. Under moderate to severe stress, stomata on the adaxial surface closed completely... |
Palavras-Chave: |
Cultivar; Estresse hidrico; Soybean. |
Thesagro: |
Soja. |
Thesaurus Nal: |
cultivars; water stress. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01565naa a2200205 a 4500 001 1077054 005 1996-05-13 008 1985 bl --- 0-- u #d 100 1 $aVILLALOBOS-RODRIGUEZ, E. 245 $aResponse of determinate and indeterminate tropical soybean cultivars to water stress. 260 $c1985 520 $aField and glasshouse experiments were conducted at the University of Costa Rica experiment station to test the hypothesis that indeterminatetropical soybean (Glycine max L.Merr) cultivars could better tolerate moisture stress than determinate types. In the field (dry season 1982)stress treatments, consisting of withholding water for 15 days, were given before flowering and during the R2 (full bloom) and the R3 (early podding) stages of plant development. In the glasshouse (August-nobember, 1982) stress treatments, consisting of withholding water for 10 days, were imposed preflowering,R2, R4 (mid-podding) and R6 (mid-seedfilling). Field-grown plants under stress had predawn leaf water potentials that were 0,1 - 0,2 MPa less than those of well-watered control plants 7 days after withholding water began. Stomata showed the most rapid decline in water vapor conductance at leaf water potential between -1,2 and - 1,4 MPa. Under moderate to severe stress, stomata on the adaxial surface closed completely... 650 $acultivars 650 $awater stress 650 $aSoja 653 $aCultivar 653 $aEstresse hidrico 653 $aSoybean 700 1 $aSHIBLES, R. 773 $tField Crops Research$gv.10, p.269-281, 1985.
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Embrapa Meio Norte / UEP-Parnaíba (CPAMN-UEPP) |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
18/03/2016 |
Data da última atualização: |
14/02/2018 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
GOMES, J. M.; NAKAYAMA, T. J.; REIS, R. R.; RODRIGUES, F. A.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; MOLINARI, H. B. C.; HARMON, F.; NEPOMUCENO, A. L. |
Afiliação: |
JULIANA MARCOLINO GOMES, UEL; THIAGO J. NAKAYAMA, UFV; RAFAELA RIBEIRO REIS, UEL; FABIANA APARECIDA RODRIGUES; LILIANE MARCIA MERTZ HENNING, CNPSO; RENATA FUGANTI PAGLIARINI; HUGO BRUNO CORREA MOLINARI, CNPAE; FRANK HARMON, USDA; ALEXANDRE LIMA NEPOMUCENO, SRI. |
Título: |
Gene expression and functional analysis of soybean genes with diurnal oscillation during drought stress. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: PLANT & ANIMAL GENOME XXIII, 2015, San Diego. |
Páginas: |
PO 203 |
Idioma: |
Inglês |
Conteúdo: |
Drought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants survival in response to drought. Together our results suggest that we identified key genes in drought tolerance. Further studies of the transgenic plants will help to unveil the drought tolerance mechanisms, contributing to the development of drought tolerant soybeans. MenosDrought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants ... Mostrar Tudo |
Thesagro: |
Gene; Resistência à seca; Soja. |
Thesaurus NAL: |
Soybeans; Water stress. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141414/1/Gene-expression-and-functional-...-2015.html
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Marc: |
LEADER 02573nam a2200277 a 4500 001 2041429 005 2018-02-14 008 2015 bl uuuu u00u1 u #d 100 1 $aGOMES, J. M. 245 $aGene expression and functional analysis of soybean genes with diurnal oscillation during drought stress.$h[electronic resource] 260 $aIn: PLANT & ANIMAL GENOME XXIII, 2015, San Diego.$c2015 300 $aPO 203 520 $aDrought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants survival in response to drought. Together our results suggest that we identified key genes in drought tolerance. Further studies of the transgenic plants will help to unveil the drought tolerance mechanisms, contributing to the development of drought tolerant soybeans. 650 $aSoybeans 650 $aWater stress 650 $aGene 650 $aResistência à seca 650 $aSoja 700 1 $aNAKAYAMA, T. J. 700 1 $aREIS, R. R. 700 1 $aRODRIGUES, F. A. 700 1 $aMERTZ-HENNING, L. M. 700 1 $aFUGANTI-PAGLIARINI, R. 700 1 $aMOLINARI, H. B. C. 700 1 $aHARMON, F. 700 1 $aNEPOMUCENO, A. L.
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Embrapa Soja (CNPSO) |
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