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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
27/11/2018 |
Data da última atualização: |
11/03/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PFEIFER, L. F. M.; RODRIGUES, W. B.; SILVA, K. C. da; ANACHE, N. A.; CASTRO, N. A.; CASTILHO, E. M.; NOGUEIRA, E. |
Afiliação: |
LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; DCR FUNDECT; UFMS; UFMS; UNIVERSIDADE FEDERAL DE PELOTAS; UNIVERSIDADE FEDERAL DE PELOTAS; ERIKLIS NOGUEIRA, CPAP. |
Título: |
Different protocols using PGF2a as ovulation inducer in nelore cows subjected to estradiol-progesterone timed AI based protocols. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Theriogenology, v. 120, p. 56-60, 2018. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2018.06.030 |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to evaluate the effect of a PGF2?-analogue (PGF) on ovulation and pregnancy rates after timed artificial insemination (TAI) in cattle. In Experiment 1 cows received an intravaginal progesterone-releasing device (CIDR) plus 2 mg im of estradiol benzoate (EB) on Day 0. The CIDR devices were removed on Day 8, and all cows received 150 ?g im of d-cloprostenol (PGF2?-analogue), 300 IU of eCG and 1 mg of estradiol cypionate (ECP) im. On Day 9, cows were randomly assigned into two groups: 1) ECP Group (n = 17), that did not receive any further treatment; and 2) ECP-PG Group (n = 14) that were given 150 ug of d-cloprostenol (PGF) as adjuvant stimulus for ovulation. No difference between groups was detected in interval for ovulation (P = 0.5), and in the proportion of cows ovulating (P = 0.09). In Experiment 2, multiparous suckling crossbred Aberdeen Angus cows (n = 260), were treated into two groups, similarly as Experiment 1; ECP group (n = 122), and ECP-PG group (n = 138). All females were TAI on Day 10. The proportion of cows treated with ECP that became pregnant was 54.9% (67/122), and cows treated with ECP plus PGF was 55.1% (76/138; P = 0.9). In Experiment 3, 686 Nelore cows, 40 to 50 days postpartum, were treated as Experiment 1 (ECP group), however, on Day 8 cows were divided into 3 groups: ECP Group (n = 216); PGF-SC Group (n = 228), in which cows did not receive ECP and were given an additional subcutaneous injection of PGF on Day 8; and PGF-IM Group (n = 242), in which cows also did not receive ECP on Day 8 and were given an additional injection of PGF im on Day 9. On Day 10, estrus was evaluated at timed AI (TAI). There was no difference in the diameter of the dominant follicle at CIDR removal and at TAI, and pregnancy per AI among groups (P > 0.05). However, the proportion of cows that displayed estrus between CIDR removal and TAI was higher in ECP group than in PGF-SC and PGF-IM groups (P < 0.001). Cows that displayed estrus has higher P/AI than cows that did not (P = 0.008). In conclusion, these results suggested that intramuscular or subcutaneous injection of PGF2? could be successfully used to induce ovulation in cattle undergoing TAI, with similar pregnancy rates when compared with ECP. The subcutaneous injection of PGF on the same day of CIDR removal could be an interesting alternative due it reduces cattle management to obtain similar results. MenosThe objective of this study was to evaluate the effect of a PGF2?-analogue (PGF) on ovulation and pregnancy rates after timed artificial insemination (TAI) in cattle. In Experiment 1 cows received an intravaginal progesterone-releasing device (CIDR) plus 2 mg im of estradiol benzoate (EB) on Day 0. The CIDR devices were removed on Day 8, and all cows received 150 ?g im of d-cloprostenol (PGF2?-analogue), 300 IU of eCG and 1 mg of estradiol cypionate (ECP) im. On Day 9, cows were randomly assigned into two groups: 1) ECP Group (n = 17), that did not receive any further treatment; and 2) ECP-PG Group (n = 14) that were given 150 ug of d-cloprostenol (PGF) as adjuvant stimulus for ovulation. No difference between groups was detected in interval for ovulation (P = 0.5), and in the proportion of cows ovulating (P = 0.09). In Experiment 2, multiparous suckling crossbred Aberdeen Angus cows (n = 260), were treated into two groups, similarly as Experiment 1; ECP group (n = 122), and ECP-PG group (n = 138). All females were TAI on Day 10. The proportion of cows treated with ECP that became pregnant was 54.9% (67/122), and cows treated with ECP plus PGF was 55.1% (76/138; P = 0.9). In Experiment 3, 686 Nelore cows, 40 to 50 days postpartum, were treated as Experiment 1 (ECP group), however, on Day 8 cows were divided into 3 groups: ECP Group (n = 216); PGF-SC Group (n = 228), in which cows did not receive ECP and were given an additional subcutaneous injection of PGF on Day 8; and PGF... Mostrar Tudo |
Palavras-Chave: |
Gametes. |
Thesagro: |
Gado Nelore; Ovulação. |
Thesaurus Nal: |
beef cattle; ovulation. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03245naa a2200265 a 4500 001 2100203 005 2019-03-11 008 2018 bl --- 0-- u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2018.06.030$2DOI 100 1 $aPFEIFER, L. F. M. 245 $aDifferent protocols using PGF2a as ovulation inducer in nelore cows subjected to estradiol-progesterone timed AI based protocols.$h[electronic resource] 260 $c2018 520 $aThe objective of this study was to evaluate the effect of a PGF2?-analogue (PGF) on ovulation and pregnancy rates after timed artificial insemination (TAI) in cattle. In Experiment 1 cows received an intravaginal progesterone-releasing device (CIDR) plus 2 mg im of estradiol benzoate (EB) on Day 0. The CIDR devices were removed on Day 8, and all cows received 150 ?g im of d-cloprostenol (PGF2?-analogue), 300 IU of eCG and 1 mg of estradiol cypionate (ECP) im. On Day 9, cows were randomly assigned into two groups: 1) ECP Group (n = 17), that did not receive any further treatment; and 2) ECP-PG Group (n = 14) that were given 150 ug of d-cloprostenol (PGF) as adjuvant stimulus for ovulation. No difference between groups was detected in interval for ovulation (P = 0.5), and in the proportion of cows ovulating (P = 0.09). In Experiment 2, multiparous suckling crossbred Aberdeen Angus cows (n = 260), were treated into two groups, similarly as Experiment 1; ECP group (n = 122), and ECP-PG group (n = 138). All females were TAI on Day 10. The proportion of cows treated with ECP that became pregnant was 54.9% (67/122), and cows treated with ECP plus PGF was 55.1% (76/138; P = 0.9). In Experiment 3, 686 Nelore cows, 40 to 50 days postpartum, were treated as Experiment 1 (ECP group), however, on Day 8 cows were divided into 3 groups: ECP Group (n = 216); PGF-SC Group (n = 228), in which cows did not receive ECP and were given an additional subcutaneous injection of PGF on Day 8; and PGF-IM Group (n = 242), in which cows also did not receive ECP on Day 8 and were given an additional injection of PGF im on Day 9. On Day 10, estrus was evaluated at timed AI (TAI). There was no difference in the diameter of the dominant follicle at CIDR removal and at TAI, and pregnancy per AI among groups (P > 0.05). However, the proportion of cows that displayed estrus between CIDR removal and TAI was higher in ECP group than in PGF-SC and PGF-IM groups (P < 0.001). Cows that displayed estrus has higher P/AI than cows that did not (P = 0.008). In conclusion, these results suggested that intramuscular or subcutaneous injection of PGF2? could be successfully used to induce ovulation in cattle undergoing TAI, with similar pregnancy rates when compared with ECP. The subcutaneous injection of PGF on the same day of CIDR removal could be an interesting alternative due it reduces cattle management to obtain similar results. 650 $abeef cattle 650 $aovulation 650 $aGado Nelore 650 $aOvulação 653 $aGametes 700 1 $aRODRIGUES, W. B. 700 1 $aSILVA, K. C. da 700 1 $aANACHE, N. A. 700 1 $aCASTRO, N. A. 700 1 $aCASTILHO, E. M. 700 1 $aNOGUEIRA, E. 773 $tTheriogenology$gv. 120, p. 56-60, 2018.
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Embrapa Pantanal (CPAP) |
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Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
28/12/2016 |
Data da última atualização: |
28/12/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
SILVEIRA, S. S.; SCHUHLI, G. S. e; DEGENHARDT-GOLDBACH, J.; QUOIRIN, M. G. G. |
Afiliação: |
SHEILA SUSY SILVEIRA, UNIVERSIDADE FEDERAL DO PARANÁ; GUILHERME SCHNELL E SCHUHLI, CNPF; JULIANA DEGENHARDT GOLDBACH, CNPF; MARGUERITE GERMAINE GHISLAINE QUOIRIN, UNIVERSIDADE FEDERAL DO PARANÁ. |
Título: |
Analysis of in vitro rooting capacity of Calophyllum brasiliense through RAPD markers. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Scientia Forestalis, Piracicaba, v. 44, n. 110, p. 527-535, jun. 2016. |
DOI: |
dx.doi.org/10.18671/scifor.v44n110.25 |
Idioma: |
Inglês |
Conteúdo: |
Marcadores RAPD (Random amplified polymorphic DNA) são simples, eficientes, baratos e não requerem conhecimento prévio do genoma do organismo estudado, o que é particularmente importante para espécies nativas. O objetivo deste estudo é identificar marcadores moleculares que possam ser usados para caracterizar genótipos responsivos às auxinas usadas para induzir o enraizamento in vitro de explantes de guanandi (Calophyllum brasiliense). Amostras de DNA foram extraídas de folhas de plantas mantidas em casa de vegetação e plantas micropropagadas após a fase de enraizamento. Quatro primers foram usados e 26 loci encontrados, dois quais 21 são polimórficos (87.5%), com um número de alelos observado de 1.87 e efetivo de 1.39. A diversidade genética de Nei foi 0.24, valor similar ao encontrado em populações naturais. A substituição de nitrogênio líquido por armazenamento a -80°C por meia hora, com a adição de polivinilpirrolidona durante a maceração, foi efetiva na prevenção da degradação das amostras. Os resultados das análises sugerem uma interação entre o genótipo e a resposta à auxina usada durante a cultura in vitro, bem como a possibilidade de um grupo de plantas particularmente responsivo. Novas técnicas são necessárias para determinar a presença de um marcador que identifique genótipos mais suscetíveis ao enraizamento in vitro. |
Palavras-Chave: |
Guanandi; Polivinilpirrolidona; RAPD. |
Thesagro: |
Cultura In Vitro; Marcador Molecular. |
Thesaurus NAL: |
Calophyllum brasiliense. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/152452/1/cap25.pdf
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Marc: |
LEADER 02154naa a2200241 a 4500 001 2059482 005 2016-12-28 008 2016 bl uuuu u00u1 u #d 024 7 $adx.doi.org/10.18671/scifor.v44n110.25$2DOI 100 1 $aSILVEIRA, S. S. 245 $aAnalysis of in vitro rooting capacity of Calophyllum brasiliense through RAPD markers.$h[electronic resource] 260 $c2016 520 $aMarcadores RAPD (Random amplified polymorphic DNA) são simples, eficientes, baratos e não requerem conhecimento prévio do genoma do organismo estudado, o que é particularmente importante para espécies nativas. O objetivo deste estudo é identificar marcadores moleculares que possam ser usados para caracterizar genótipos responsivos às auxinas usadas para induzir o enraizamento in vitro de explantes de guanandi (Calophyllum brasiliense). Amostras de DNA foram extraídas de folhas de plantas mantidas em casa de vegetação e plantas micropropagadas após a fase de enraizamento. Quatro primers foram usados e 26 loci encontrados, dois quais 21 são polimórficos (87.5%), com um número de alelos observado de 1.87 e efetivo de 1.39. A diversidade genética de Nei foi 0.24, valor similar ao encontrado em populações naturais. A substituição de nitrogênio líquido por armazenamento a -80°C por meia hora, com a adição de polivinilpirrolidona durante a maceração, foi efetiva na prevenção da degradação das amostras. Os resultados das análises sugerem uma interação entre o genótipo e a resposta à auxina usada durante a cultura in vitro, bem como a possibilidade de um grupo de plantas particularmente responsivo. Novas técnicas são necessárias para determinar a presença de um marcador que identifique genótipos mais suscetíveis ao enraizamento in vitro. 650 $aCalophyllum brasiliense 650 $aCultura In Vitro 650 $aMarcador Molecular 653 $aGuanandi 653 $aPolivinilpirrolidona 653 $aRAPD 700 1 $aSCHUHLI, G. S. e 700 1 $aDEGENHARDT-GOLDBACH, J. 700 1 $aQUOIRIN, M. G. G. 773 $tScientia Forestalis, Piracicaba$gv. 44, n. 110, p. 527-535, jun. 2016.
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Embrapa Florestas (CNPF) |
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