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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
23/03/2016 |
Data da última atualização: |
23/03/2016 |
Autoria: |
ALCÂNTARA, B. K. de; ALFIERI, A. A.; HEADLEY, S. A.; RODRIGUES, W. B.; OTONEL, R. A. A.; LUNARDI, M.; ALFIERI, A. F. |
Afiliação: |
BRÍGIDA K. DE ALCÂNTARA, DMVP/UEL; AMAURI A. ALFIERI, DMVP/UEL; SELWYN A. HEADLEY, DMVP/UEL; WAGNER B. RODRIGUES, DMVP/UEL; RODRIGO A. A. OTONEL, DMVP/UEL; MICHELE LUNARDI, UNIC; ALICE F. ALFIERI, DMVP/UEL. |
Título: |
Molecular characterization of bovine Deltapapillomavirus (BPV1, 2, and 13) DNA in equine sarcoids. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 431-436, maio 2015. |
Idioma: |
Inglês |
Conteúdo: |
Sarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreover, these findings represent the first description of three types of BPV diagnosed in the same horse, as well as the first confirmation of BPV1 and 2 in horses from Brazil. MenosSarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreove... Mostrar Tudo |
Palavras-Chave: |
Bovine Deltapapillomavirus; Deltapapillomavirus bovino; Equine sarcoids; Horse; Papillomavirus; Sarcoide equino; Tumor de pele. |
Thesagro: |
DNA. |
Thesaurus Nal: |
Papillomaviridae; Skin neoplasms. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141614/1/Molecular-characterization.pdf
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Marc: |
LEADER 02588naa a2200313 a 4500 001 2041730 005 2016-03-23 008 2015 bl uuuu u00u1 u #d 100 1 $aALCÂNTARA, B. K. de 245 $aMolecular characterization of bovine Deltapapillomavirus (BPV1, 2, and 13) DNA in equine sarcoids. 260 $c2015 520 $aSarcoids are fibroblastic lesions, which are considered as the most common skin tumors of horses; spontaneous regression rarely occurs. The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. This study characterized the DNA of BPVs in sarcoids from 15 horses from Brazil by analyzing 20 cutaneous lesions (12 recently collected; 8 from formalin-fixed paraffin-embedded (FFPE) tissues). Histopathology confirmed the proliferative lesions as sarcoids. Three PCRs were performed to amplify papillomavirus (PV) DNA. For screening, the primers IFNR2/IDNT2 were used to amplify a fragment of the PV L1 ORF. The second primer set was complementary to a common sequence of the E5L2 genomic region of BPV1, 2, and 13. The third primer pair (FAP59/FAP64) targeted a fragment of the PVs L1 ORF. The screening and E5L2 PCRs yielded amplicons in all samples evaluated. The FAP amplicons identified BPV1, 2, and 13 only from fresh tissue samples. The phylogenetic analyses of E5L2 resulted in the identification of BPV1, 2, and 13 in 14 (70%), 2 (10%), and 4 (20%) sarcoids, respectively. Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). This study confirmed the presence of BPV1, 2, and 13 DNA in equine sarcoids. Moreover, these findings represent the first description of three types of BPV diagnosed in the same horse, as well as the first confirmation of BPV1 and 2 in horses from Brazil. 650 $aPapillomaviridae 650 $aSkin neoplasms 650 $aDNA 653 $aBovine Deltapapillomavirus 653 $aDeltapapillomavirus bovino 653 $aEquine sarcoids 653 $aHorse 653 $aPapillomavirus 653 $aSarcoide equino 653 $aTumor de pele 700 1 $aALFIERI, A. A. 700 1 $aHEADLEY, S. A. 700 1 $aRODRIGUES, W. B. 700 1 $aOTONEL, R. A. A. 700 1 $aLUNARDI, M. 700 1 $aALFIERI, A. F. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 35, n. 5, p. 431-436, maio 2015.
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Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
01/03/2013 |
Data da última atualização: |
25/04/2013 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CAPALBO, D. M. F.; PAULA, D. P.; MENDES, S. M.; SUJII, E. R.; PIRES, C. S. S.; ARANTES, O. M. N. |
Afiliação: |
DEISE MARIA FONTANA CAPALBO, CNPMA; DEBORA PIRES PAULA, CENARGEN; SIMONE MARTINS MENDES, CNPMS; EDISON RYOITI SUJII, CENARGEN; CARMEN SILVIA SOARES PIRES, CENARGEN; O. M. N. ARANTES, Consultora - LAC-Biosafety Project. |
Título: |
Environmental safety, socioeconomics and public awareness of GMOs in Brazil: the context of LAC-Biosafety Project. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON BIOSAFETY OF GENETICALLY MODIFIED ORGANISMS, 12., Saint Louis, 2012. Saint Louis: The Donald Danforth Plant Science Center, 2012. Nº P1.49. |
Idioma: |
Inglês |
Palavras-Chave: |
Environmental safety; GMOs in Brazil; LAC-Biosafety Project; Public awareness; Socioeconomics awareness. |
Thesaurus NAL: |
Genetically modified organisms. |
Categoria do assunto: |
S Ciências Biológicas |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77743/1/RA-CapalboDMF-ISBGMO2012-P1.49.pdf
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Marc: |
LEADER 00885nam a2200229 a 4500 001 1951888 005 2013-04-25 008 2012 bl uuuu u00u1 u #d 100 1 $aCAPALBO, D. M. F. 245 $aEnvironmental safety, socioeconomics and public awareness of GMOs in Brazil$bthe context of LAC-Biosafety Project.$h[electronic resource] 260 $aIn: INTERNATIONAL SYMPOSIUM ON BIOSAFETY OF GENETICALLY MODIFIED ORGANISMS, 12., Saint Louis, 2012. Saint Louis: The Donald Danforth Plant Science Center, 2012. Nº P1.49.$c2012 650 $aGenetically modified organisms 653 $aEnvironmental safety 653 $aGMOs in Brazil 653 $aLAC-Biosafety Project 653 $aPublic awareness 653 $aSocioeconomics awareness 700 1 $aPAULA, D. P. 700 1 $aMENDES, S. M. 700 1 $aSUJII, E. R. 700 1 $aPIRES, C. S. S. 700 1 $aARANTES, O. M. N.
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