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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
28/01/2021 |
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Data da última atualização: |
10/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, F. C. B. das; CUNHA, E. F. M.; RODRIGUES, S. de M.; BONFIM, K.; TAVARES, E. J. M.; MOURA, M. F.; SILVA, G. F. da. |
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Afiliação: |
Francisca Chagas Bezerra das Araújo, UFRA; ELISA FERREIRA MOURA CUNHA, CPATU; SIMONE DE MIRANDA RODRIGUES, CPATU; KENNY BONFIM DE ARRUDA CARVALHO, Cenargen; ERALDO JOSE MADUREIRA TAVARES, CPATSA; Mônika Fecury Moura, CPATU; GILVAN FERREIRA DA SILVA, CPAA. |
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Título: |
Identification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Genetic Resources and Crop Evolution, v. 68, p. 1809-1824, 2021. |
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DOI: |
https://doi.org/10.1007/s10722-020-01097-x |
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Idioma: |
Inglês |
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Conteúdo: |
The biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally. MenosThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. T... Mostrar Tudo |
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Palavras-Chave: |
Biofortificação; Raíz rica em carotenoide. |
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Thesagro: |
Carotenóide; Mandioca; Manihot Esculenta; Polimorfismo; Proteína; Raiz Alimentícia; Variação Genética. |
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Thesaurus Nal: |
Carotenoids; Cassava; Haplotypes; Polymorphism. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 02740naa a2200361 a 4500
001 2129648
005 2021-06-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10722-020-01097-x$2DOI
100 1 $aARAÚJO, F. C. B. das
245 $aIdentification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon.$h[electronic resource]
260 $c2021
520 $aThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally.
650 $aCarotenoids
650 $aCassava
650 $aHaplotypes
650 $aPolymorphism
650 $aCarotenóide
650 $aMandioca
650 $aManihot Esculenta
650 $aPolimorfismo
650 $aProteína
650 $aRaiz Alimentícia
650 $aVariação Genética
653 $aBiofortificação
653 $aRaíz rica em carotenoide
700 1 $aCUNHA, E. F. M.
700 1 $aRODRIGUES, S. de M.
700 1 $aBONFIM, K.
700 1 $aTAVARES, E. J. M.
700 1 $aMOURA, M. F.
700 1 $aSILVA, G. F. da
773 $tGenetic Resources and Crop Evolution$gv. 68, p. 1809-1824, 2021.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
04/07/2007 |
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Data da última atualização: |
19/02/2016 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
MELO FILHO, G. A. de; COSTA, F. P.; CORRÊA, E. S.; PEREIRA, M. de A.; CEZAR, I. M.; NETTO, F. G. da S. |
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Afiliação: |
Embrapa Gado de Corte (Campo Grande, MS). |
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Título: |
Sistema e custo de produção de gado de corte no Estado de Rondônia. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Campo Grande, MS: Embrapa Gado de Corte, 2005. |
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Páginas: |
7 p. |
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Série: |
(Embrapa Gado de Corte. Comunicado Técnico, 92). |
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Idioma: |
Português |
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Notas: |
CNPGC. |
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Conteúdo: |
Panaroma da pecuária de corte em Rondônia. Descrição do sistema de produção de gado de corte em Rondônia. Caracterização da região. Síntese do sistema |
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Palavras-Chave: |
Bovino de corte; Brasil; Costs; Rondônia. |
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Thesagro: |
Análise Econômica; Custo; Sistema de Produção. |
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Thesaurus NAL: |
beef cattle; Brazil; economic analysis; farming systems. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139531/1/COT-92.pdf
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Marc: |
LEADER 01070nam a2200337 a 4500
001 1326302
005 2016-02-19
008 2005 bl uuuu u0uu1 u #d
100 1 $aMELO FILHO, G. A. de
245 $aSistema e custo de produção de gado de corte no Estado de Rondônia.
260 $aCampo Grande, MS: Embrapa Gado de Corte$c2005
300 $a7 p.
490 $a(Embrapa Gado de Corte. Comunicado Técnico, 92).
500 $aCNPGC.
520 $aPanaroma da pecuária de corte em Rondônia. Descrição do sistema de produção de gado de corte em Rondônia. Caracterização da região. Síntese do sistema
650 $abeef cattle
650 $aBrazil
650 $aeconomic analysis
650 $afarming systems
650 $aAnálise Econômica
650 $aCusto
650 $aSistema de Produção
653 $aBovino de corte
653 $aBrasil
653 $aCosts
653 $aRondônia
700 1 $aCOSTA, F. P.
700 1 $aCORRÊA, E. S.
700 1 $aPEREIRA, M. de A.
700 1 $aCEZAR, I. M.
700 1 $aNETTO, F. G. da S.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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