| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Instrumentação. |
|
Data corrente: |
24/05/2022 |
|
Data da última atualização: |
23/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
RIBEIRO, T. S.; SCRAMIN, J. A.; RODRIGUES, J. A. S.; BERNARDES FILHO, R.; COLNAGO, L. A.; FORATO, L. A. |
|
Afiliação: |
RUBENS BERNARDES FILHO, CNPDIA; LUIZ ALBERTO COLNAGO, CNPDIA; LUCIMARA APARECIDA FORATO, CNPDIA. |
|
Título: |
13C ss-NMR Singular value decomposition and fitting for sorghum proteins conformation elucidation. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Polímeros, v. 32, n. 1, e2022009, 2022. |
|
Páginas: |
6 p. |
|
ISSN: |
1678-5169 |
|
DOI: |
10.1590/0104-1428.20210082 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Kafirins, water-insoluble proteins from Sweet Sorghum BR501 grains, have been an alternative to prepare edible coatings for food due to their hydrophobic character. In this work, the secondary structures (SS) content of reduced (SSr) and unreduced (SSu) kafirins were determined by 13C solid-state-NMR spectroscopy using areas of carbonyl peak. The SS elucidate by fitting the signal with the Lorentzian function shows 56% and 59% of α-helix and 40% and 12% of β-sheet structures for SSr and SSu, respectively. The SS also were elucidated by a Singular value decomposition- SVD method shows 55% and 49% of α-helix and 12% and 8% of β-sheet structure for SSr and SSu, respectively. Since SVD does not depend on the operator and has higher correlation coefficients for α-helix (0.96%) and β-sheet (0.91%), it is a reliable method to quantify the SS of insoluble proteins using the 13C NMR signal. |
|
Palavras-Chave: |
13C NMR spectroscopy; Kafirin; Secondary structures proteins. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1143402/1/P-3C-ss-NMR-Singular-value-decomposition-and-fitting-for.pdf
|
|
Marc: |
LEADER 01652naa a2200253 a 4500
001 2143402
005 2024-01-23
008 2022 bl uuuu u00u1 u #d
022 $a1678-5169
024 7 $a10.1590/0104-1428.20210082$2DOI
100 1 $aRIBEIRO, T. S.
245 $a13C ss-NMR Singular value decomposition and fitting for sorghum proteins conformation elucidation.$h[electronic resource]
260 $c2022
300 $a6 p.
520 $aKafirins, water-insoluble proteins from Sweet Sorghum BR501 grains, have been an alternative to prepare edible coatings for food due to their hydrophobic character. In this work, the secondary structures (SS) content of reduced (SSr) and unreduced (SSu) kafirins were determined by 13C solid-state-NMR spectroscopy using areas of carbonyl peak. The SS elucidate by fitting the signal with the Lorentzian function shows 56% and 59% of α-helix and 40% and 12% of β-sheet structures for SSr and SSu, respectively. The SS also were elucidated by a Singular value decomposition- SVD method shows 55% and 49% of α-helix and 12% and 8% of β-sheet structure for SSr and SSu, respectively. Since SVD does not depend on the operator and has higher correlation coefficients for α-helix (0.96%) and β-sheet (0.91%), it is a reliable method to quantify the SS of insoluble proteins using the 13C NMR signal.
653 $a13C NMR spectroscopy
653 $aKafirin
653 $aSecondary structures proteins
700 1 $aSCRAMIN, J. A.
700 1 $aRODRIGUES, J. A. S.
700 1 $aBERNARDES FILHO, R.
700 1 $aCOLNAGO, L. A.
700 1 $aFORATO, L. A.
773 $tPolímeros$gv. 32, n. 1, e2022009, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 1 | |
| 1. |  | FACCHINATTO, W. M.; GALANTE, J.; MESQUITA, L.; SILVA, D. S.; SANTOS, D. M. dos; MORAES, T. B.; CAMPANA-FILHO, S. P.; COLNAGO, L. A.; SARMENTO, B.; NEVES, J. das. Clotrimazole-loaded N-(2-hydroxy)-propyl-3-trimethylammonium, O-palmitoyl chitosan nanoparticles for topical treatment of vulvovaginal candidiasis. Acta Biomaterialia, v. 125, 2021. 312?321| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
|    |
| Registros recuperados : 1 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
