| |
|
|
| Registros recuperados : 4 | |
| 2. |  | ALMEIDA, A. de S.; PAIXÃO, M. R. R.; RODRIGUES, F. G.; OLIVEIRA JÚNIOR, W. G. de; LOBO JUNIOR, M. Comparação de testes de vigor de sementes de feijão-comum por análise visual e processamento de imagens com o software Image J. In: SEMINÁRIO JOVENS TALENTOS, 16., 2022, Santo Antônio de Goiás. Resumos... Brasília, DF: Embrapa; Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2022. p. 72.| Biblioteca(s): Embrapa Arroz e Feijão. |
|    |
| 3. | | RODRIGUES, F. G.; ALMEIDA, A. de S.; OLIVEIRA JUNIOR, W. G. de; BARBOSA, E. T.; FILIPPI, M. C. C. de; LOBO JUNIOR, M. Bioensaio para avaliação de Bacillus spp. no controle biológico de microescleródios de Macrophomina phaseolina. In: SEMINÁRIO JOVENS TALENTOS, 16., 2022, Santo Antônio de Goiás. Resumos... Brasília, DF: Embrapa; Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2022. p. 33.| Biblioteca(s): Embrapa Arroz e Feijão. |
| |
| 4. | | OLIVEIRA JÚNIOR, W. G. de; ALMEIDA, A. de S.; RODRIGUES, F. G.; MARTINS, I. R.; BARBOSA, E. T.; LOBO JUNIOR, M. Efeito inibidor de metabólitos não voláteis de Trichoderma spp. contra patógenos causadores de doenças radiculares em plantas de feijoeiro-comum. In: SEMINÁRIO JOVENS TALENTOS, 16., 2022, Santo Antônio de Goiás. Resumos... Brasília, DF: Embrapa; Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2022. p. 54.| Biblioteca(s): Embrapa Arroz e Feijão. |
| |
| Registros recuperados : 4 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agrobiologia. |
|
Data corrente: |
19/12/2018 |
|
Data da última atualização: |
29/04/2019 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
SILVA, P. R. A. da; VIDAL, M. S.; SOARES, C. de P.; POLESE, V.; TADRA-SFEIR, M. Z.; SOUZA, E. M. de; ARAUJO, J. L. S. de; BALDANI, J. I. |
|
Afiliação: |
PAULA RENATA ALVES DA SILVA, BOLSISTA EMBRAPA AGROBIOLOGIA; MARCIA SOARES VIDAL, CNPAB; CLEITON DE PAULA SOARES, UFRJ; VALÉRIA POLESE, UFRJ; MICHELLE ZIBETTI TADRA-SFEIR,, UFRJ; EMANUEL MALTEMPI DE SOUZA, UFRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB. |
|
Título: |
Sugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
PLOS ONE, v. 13, n. 12, e0207863, 2018. |
|
ISSN: |
1932-6203 |
|
Idioma: |
Português |
|
Conteúdo: |
The stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of genes involved in bacterial metabolism was regulated (up and down), suggesting that the metabolism of strain Ppe8 is modulated by the apoplast fluid. These results suggest that Ppe8 alters its gene expression pattern in the presence of apoplast fluid mainly in order to use compounds present in the fluid as well as to avoid the induction of plant defense mechanisms. This is a pioneer study showing the role played by the sugarcane apoplast fluid on the global modulation of genes in P. tropica strain Ppe8. MenosThe stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of... Mostrar Tudo |
|
Palavras-Chave: |
Bacterial growth; RNA Sequence; Sugarcane variety RB867515; Transcriptomic profile. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
Marc: |
LEADER 02828naa a2200265 a 4500
001 2102134
005 2019-04-29
008 2018 bl uuuu u00u1 u #d
022 $a1932-6203
100 1 $aSILVA, P. R. A. da
245 $aSugarcane apoplast fluid modulates the global transcriptional profile of the diazotrophic bacteria Paraburkholderia tropica strain Ppe8.$h[electronic resource]
260 $c2018
520 $aThe stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression. The bacterium was grown in JMV liquid medium (100 ml), divided equally and then supplemented with 50 ml of fresh JMV medium or 50 ml of apoplast fluid extracted from sugarcane variety RB867515. Total RNA was extracted 2 hours later, the rRNAs were depleted and mRNAs used to construct libraries to sequence the fragments using Ion Torrent technology. The mapping and statistical analysis were carried out with CLC Genomics Workbench software. The RNA-seq data was validated by RT-qPCR using the reference genes fliP1, paaF, and groL. The data analysis showed that 544 genes were repressed and 153 genes were induced in the presence of apoplast fluid. Genes that induce plant defense responses, genes related to chemotaxis and movements were repressed in the presence of apoplast fluid, indicating that strain Ppe8 recognizes the apoplast fluid as a plant component. The expression of genes involved in bacterial metabolism was regulated (up and down), suggesting that the metabolism of strain Ppe8 is modulated by the apoplast fluid. These results suggest that Ppe8 alters its gene expression pattern in the presence of apoplast fluid mainly in order to use compounds present in the fluid as well as to avoid the induction of plant defense mechanisms. This is a pioneer study showing the role played by the sugarcane apoplast fluid on the global modulation of genes in P. tropica strain Ppe8.
653 $aBacterial growth
653 $aRNA Sequence
653 $aSugarcane variety RB867515
653 $aTranscriptomic profile
700 1 $aVIDAL, M. S.
700 1 $aSOARES, C. de P.
700 1 $aPOLESE, V.
700 1 $aTADRA-SFEIR, M. Z.
700 1 $aSOUZA, E. M. de
700 1 $aARAUJO, J. L. S. de
700 1 $aBALDANI, J. I.
773 $tPLOS ONE$gv. 13, n. 12, e0207863, 2018.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrobiologia (CNPAB) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
