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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CARVALHO, J. O.; MICHALCZECHEN-LACERDA, V. A.; SARTORI, R.; RODRIGUES, F. C.; BRAVIM, O.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
JOSE O. CARVALHO, UNIVERSIDADE DE SÃO PAULO; VALQUIRIA A. MICHALCZECHEN-LACERDA, UnB; ROBERTO SARTORI, UNIVERSIDADE DE SÃO PAULO; FERNANDA C. RODRIGUES; OTAVIO BRAVIM, UnB; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Reproduction e Development, v. 79, p. 77-84, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed. |
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Palavras-Chave: |
Gene IGF2; Gene IGF2R; Sexagem por citometria. |
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Thesagro: |
Bovino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179337/1/Carvalho-et-al-2012-Molecular-Reproduction-and-Development.pdf
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Marc: |
LEADER 02074nam a2200229 a 4500
001 1950725
005 2023-03-07
008 2012 bl uuuu u00u1 u #d
100 1 $aCARVALHO, J. O.
245 $aThe methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting.$h[electronic resource]
260 $aMolecular Reproduction e Development, v. 79, p. 77-84$c2012
520 $aThe objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed.
650 $aBovino
653 $aGene IGF2
653 $aGene IGF2R
653 $aSexagem por citometria
700 1 $aMICHALCZECHEN-LACERDA, V. A.
700 1 $aSARTORI, R.
700 1 $aRODRIGUES, F. C.
700 1 $aBRAVIM, O.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 51 | |
| 6. |  | MICHALCZECHEN LACERDA, V. A.; RODRIGUES, F. C.; SOUSA, R. V. de; RUMPF, R.; FRANCO, M. M. 53 Impacts of using procaine as a DNA-Demethylating agent in in vitro culture of bovine cells. Reproduction, Fertility and Development, v.23, n.1, p. 132-133, 2011. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, Orlando, Florida, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | MICHALCZECHEN-LACERDA, V. A.; RODRIGUES, F. C.; SOUSA, R. V. de; RUMPF, R.; FRANCO, M. M. Impactos do uso da procaína como agente desmetilante de DNA no cultivo celular de bovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 045.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | FREIRE, J. M.; PIÑA-RODRIGUES, F. C. M.; SANTOS, A. L. F. dos; PEREIRA, M. B. Intra-and inter-population variation in seed size and dormancy in Schizolobium parahyba (Vell.) Blake inthe Atlantic Forest. Variação entre e dentro de populações em tamanho e dormência de sementes de Schizolobium parahyba (Vell.) Blake na Floresta Atlântica Ciência Florestal, Santa Maria, v. 25, n. 4, p. 897-907, out./dez., 2015| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 16. | | GOMES, J. M.; PEREIRA, M. G.; PINA-RODRIGUES, F. C. M.; GONDIM, F. R.; SILVA, E. M. R. da. Aporte de serapilheira e de nutrientes em fragmentos florestais da Mata atlântica, RJ. Revista Brasileira de Ciências Agrárias, Recife,v. 5, n., 3, p. 383-391, jul./set. 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 17. | | PIÑA-RODRIGUES, F. C. M.; RUZEDO, D. G.; FREIRE, J. M.; PIOTROWSKI, I.; SILVA, J. M. S. da. Challenges for scaling-up native seed production to direct seeding in the Brazilian dry areas: a seed technology approach. In: WORLD CONFERENCE ON ECOLOGICAL RESTORATION, 7.; CONGRESO IBEROAMERICANO Y DEL CARIBE DE RESTAURACIÓN ECOLÓGICA, 5.; CONFERÊNCIA BRASILEIRA DE RESTAURAÇÃO ECOLÓGICA, 1., 2017, Foz do Iguassu Linking science and practice for a better world: book of abstracts. Londrina: Sociedade Brasileira de Restauração Ecológica, 2017. 448 p. p. 51| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 19. | | PIÑA-RODRIGUES, F. C. M.; FREIRE, J. M.; SILVA, J. M. S.; PIOTROWSKI, I.; ALMEIDA, L. S. O futuro das sementes nativas: inovação tecnológica para ganhos de escala. Informativo Abrates, 28, n.2, Edição Especial, 2018. P. 60 II BRASILEIRA DE RESTAURAÇÃO ECOLÓGICA; X SIMPÓSIO BRASILEIRO SOBRE TECNOLOGIA DE SEMENTES FLORESTAIS, 21 a 23 de novembro de 2018,
Belo Horizonte, MG.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 20. | | BONOMO, V. S.; RODRIGUES, F. C.; SILVA, F. L.; OLIVEIRA, A. C. B. de; PEREIRA, A. A. Divergência genética entre acessos de café conilon em Minas Gerais. In: CONGRESSO BRASILEIRO DE PESQUISA CAFEEIROAS, 36., 2010, Guarapari. Anais...Brasília, DF: Embrapa Café, 2010.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 51 | |
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