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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/08/2002 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOGUERCIO, L. L.; BARRETO, M. L.; ROCHA, T. L.; SANTOS, C. G.; TEIXEIRA, F. F.; PAIVA, E. |
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Afiliação: |
FLAVIA FRANCA TEIXEIRA, CNPMS. |
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Título: |
Combined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Journal of Applied Microbiology, Danvers, v. 93, n. 2, p. 269-277, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm. |
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Thesagro: |
Genética. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02123naa a2200193 a 4500
001 1485545
005 2018-06-07
008 2002 bl uuuu u00u1 u #d
100 1 $aLOGUERCIO, L. L.
245 $aCombined analysis of supernatant-based feeding bioassay and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm.$h[electronic resource]
260 $c2002
520 $aAims: To assess whether feeding bioassays using culture-supernatant proteins could be combined with PCR into a first-tier screening strategy for Vip3A-like genes efficient against tropical Spodoptera frugiperda. Methods and Results: Out of 12 Bacillus thuringiensis strains studied, the total protein concentrated from the culture supernatant of only the strain HD125 yielded a significantly increased armyworm mortality and an intense band of the predicted size for VIP3A protein in SDS-PAGE. However, PCR and sequencing data indicated Vip-like genes are ubiquitous in tropical B. thuringiensis isolated. Interestingly, the HD125 strain was also the only one displaying a single-band amplification pattern and the highest sequence identity to the reported Vip3A(a) gene. Conclusions: Results suggest the insecticidal effectiveness of putative VIPs in B. thuringiensis isolates can be preliminarily estimated by the use of supernatant-derived total protein in feeding experiments, though only in a limited manner. Significance and impact of the study: a simple and cost-effective first-tier screening strategy for VIP-derived activities in B. thuringiensis collections can be developed by combining PCR and feeding bioassays. Moreover, the employed primers showed to be useful as a tool forr strains differentiation at DNA level, and for characterization and isolation of Vip-like genes in tropical B. thuringiensis germplasm.
650 $aGenética
700 1 $aBARRETO, M. L.
700 1 $aROCHA, T. L.
700 1 $aSANTOS, C. G.
700 1 $aTEIXEIRA, F. F.
700 1 $aPAIVA, E.
773 $tJournal of Applied Microbiology, Danvers$gv. 93, n. 2, p. 269-277, 2002.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
24/05/1999 |
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Data da última atualização: |
06/06/2006 |
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Autoria: |
FRANCA NETO, J. B.; KRZYZANOWSKI, F. C.; COSTA, N. P. |
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Título: |
Previsao da emergencia a campo de lotes de sementes de soja, atraves dos resultados de vigor obtidos pelo teste de tetrazolio. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
In: REUNIAO DE PESQUISA DA SOJA DA REGIAO CENTRAL DO BRASIL, 20., 1998, Londrina. Ata e resumos. Londrina: EMBRAPA-CNPSo, 1998. |
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Páginas: |
p.395-396. |
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Série: |
(EMBRAPA-CNPSo. Documentos, 121). |
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Idioma: |
Português |
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Palavras-Chave: |
Brasil; Parana; Seed longevity; Soybean. |
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Thesagro: |
Semente; Soja; Teste de Tetrazólio. |
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Thesaurus NAL: |
Brazil; vigor. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00835naa a2200265 a 4500
001 1460602
005 2006-06-06
008 1998 bl uuuu u00u1 u #d
100 1 $aFRANCA NETO, J. B.
245 $aPrevisao da emergencia a campo de lotes de sementes de soja, atraves dos resultados de vigor obtidos pelo teste de tetrazolio.
260 $c1998
300 $ap.395-396.
490 $a(EMBRAPA-CNPSo. Documentos, 121).
650 $aBrazil
650 $avigor
650 $aSemente
650 $aSoja
650 $aTeste de Tetrazólio
653 $aBrasil
653 $aParana
653 $aSeed longevity
653 $aSoybean
700 1 $aKRZYZANOWSKI, F. C.
700 1 $aCOSTA, N. P.
773 $tIn: REUNIAO DE PESQUISA DA SOJA DA REGIAO CENTRAL DO BRASIL, 20., 1998, Londrina. Ata e resumos. Londrina: EMBRAPA-CNPSo, 1998.
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