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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
22/04/2019 |
Data da última atualização: |
24/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FERREIRA, M. T. M.; ROCHA, L. C.; VITORIANO, M. B. Z.; MITTELMANN, A.; TECHIO, V. H. |
Afiliação: |
Marco Tulio Mendes Ferreira, UFLA; Laiane Corsini Rocha, UFLA; Matheus Braga Zanon Vitoriano, UFLA; ANDREA MITTELMANN, CNPGL; Vânia Helena Techio, UFLA. |
Título: |
Relationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Molecular Biology Reports, v. 45, p. 1663-1679, 2018. |
DOI: |
10.1007/s11033-018-4310-9 |
Idioma: |
Inglês |
Conteúdo: |
Abstract The grasses of the Lolium?Festuca complex show a prominent role in world agricultural scenario. Several studies have demonstrated that the plasticity of 45S rDNA sites has been recently associated with the possible fragility of the loci. Often, these fragile sites were observed as extended sites and gaps in metaphases. This organization can be evaluated in relation to their transcriptional activity/accessibility through epigenetic changes. Thus, this study aimed to investigate the relationship of the 5-methylcytosine and histone H3 lysine-9 dimethylation in different conformations of 45S rDNA sites in interphase nuclei and in metaphase chromosomes of L. perenne, L. multiflorum and F. arundinacea. The FISH technique using 45S rDNA probes was performed sequentially after the immunolocalization. The sites showed predominantly the following characteristics in the interphase nuclei: intra- and perinucleolar position, decondensed or partially condensed and hypomethylated and hyper/hypomethylated status. Extranucleolar sites were mainly hypermethylated for both epigenetic marks. The 45S rDNA sites with gaps identified in metaphases were always hypomethylated, which justifies it decondensed and transcriptional state. The frequency of sites with hypermethylated gaps was very low. The structural differences observed in these sites are directly related to the assessed epigenetic marks, justifying the different conformations throughout the cell cycle. |
Palavras-Chave: |
Epigenetic marks; Fragile sites; Lolium Festuca complex; Post translational modification. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02236naa a2200229 a 4500 001 2108357 005 2023-01-24 008 2018 bl uuuu u00u1 u #d 024 7 $a10.1007/s11033-018-4310-9$2DOI 100 1 $aFERREIRA, M. T. M. 245 $aRelationship between epigenetic marks and the behavior of 45S rDNA sites in chromosomes and interphase nuclei of Lolium-Festuca complex.$h[electronic resource] 260 $c2018 520 $aAbstract The grasses of the Lolium?Festuca complex show a prominent role in world agricultural scenario. Several studies have demonstrated that the plasticity of 45S rDNA sites has been recently associated with the possible fragility of the loci. Often, these fragile sites were observed as extended sites and gaps in metaphases. This organization can be evaluated in relation to their transcriptional activity/accessibility through epigenetic changes. Thus, this study aimed to investigate the relationship of the 5-methylcytosine and histone H3 lysine-9 dimethylation in different conformations of 45S rDNA sites in interphase nuclei and in metaphase chromosomes of L. perenne, L. multiflorum and F. arundinacea. The FISH technique using 45S rDNA probes was performed sequentially after the immunolocalization. The sites showed predominantly the following characteristics in the interphase nuclei: intra- and perinucleolar position, decondensed or partially condensed and hypomethylated and hyper/hypomethylated status. Extranucleolar sites were mainly hypermethylated for both epigenetic marks. The 45S rDNA sites with gaps identified in metaphases were always hypomethylated, which justifies it decondensed and transcriptional state. The frequency of sites with hypermethylated gaps was very low. The structural differences observed in these sites are directly related to the assessed epigenetic marks, justifying the different conformations throughout the cell cycle. 653 $aEpigenetic marks 653 $aFragile sites 653 $aLolium Festuca complex 653 $aPost translational modification 700 1 $aROCHA, L. C. 700 1 $aVITORIANO, M. B. Z. 700 1 $aMITTELMANN, A. 700 1 $aTECHIO, V. H. 773 $tMolecular Biology Reports$gv. 45, p. 1663-1679, 2018.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
19/09/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
Autoria: |
KARAM, D.; OLIVEIRA, M. F. de; PEREIRA FILHO, I. A.; JESUS, L. L. |
Afiliação: |
DECIO KARAM, CNPMS; MAURILIO FERNANDES DE OLIVEIRA, CNPMS; ISRAEL ALEXANDRE PEREIRA FILHO, CNPMS; Liliane L. Jesus, Centro Universitário de Sete Lagoas. |
Título: |
Sensibilidade de híbridos de milho e herbicidas pós-emergentes aplicados em dois estádios fenológicos da cultura. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 27.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 3.; WORKSHOP SOBRE MANEJO E ETIOLOGIA DA MANCHA BRANCA DO MILHO, 2008, Londrina. Agroenergia, produção de alimentos e mudanças climáticas: desafios para milho e sorgo: trabalhos e palestras. [Londrina]: IAPAR; [Sete Lagoas]: Embrapa Milho e Sorgo, 2008. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Sulfoniluréia; Triketona. |
Thesagro: |
Fitotoxicidade; Zea Mays. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/30017/1/Sensibilidade-hibridos.pdf
|
Marc: |
LEADER 00936nam a2200193 a 4500 001 1491280 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 100 1 $aKARAM, D. 245 $aSensibilidade de híbridos de milho e herbicidas pós-emergentes aplicados em dois estádios fenológicos da cultura.$h[electronic resource] 260 $aIn: CONGRESSO NACIONAL DE MILHO E SORGO, 27.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 3.; WORKSHOP SOBRE MANEJO E ETIOLOGIA DA MANCHA BRANCA DO MILHO, 2008, Londrina. Agroenergia, produção de alimentos e mudanças climáticas: desafios para milho e sorgo: trabalhos e palestras. [Londrina]: IAPAR; [Sete Lagoas]: Embrapa Milho e Sorgo$c2008 300 $c1 CD-ROM. 650 $aFitotoxicidade 650 $aZea Mays 653 $aSulfoniluréia 653 $aTriketona 700 1 $aOLIVEIRA, M. F. de 700 1 $aPEREIRA FILHO, I. A. 700 1 $aJESUS, L. L.
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