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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
03/04/1995 |
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Data da última atualização: |
04/04/2017 |
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Autoria: |
KRETTLI, A. U.; LAURENTE, K. E.; DAHER, V. R.; ROCHA, E. M. M. da. |
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Título: |
Immunogenicity and infectivity of mature and imature plasmodium gallinaceum sporozoites. |
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Ano de publicação: |
1986 |
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Fonte/Imprenta: |
Memorias Instituto Owaldo Cruz, v.81, p.115-122, 1986. Supplement II. |
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Idioma: |
Inglês |
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Conteúdo: |
The infectivity and immunogenicity of two different populations of Plamodium gallinaceum sporozoites and their reactivity in vitro with normal and specific immune sera were studied in paralel experiments. Sporozoites from salivary glands (SGS) or from oocysis (OoS) contained in midguts of Aedes fluviatilis mosquitos gradually acquired infectivity to chiks regardless of their location. This infectivity was abolished after parasite exposure for 30 min to ultraviolete (UV) lights or to X-rays (13Krads). Inactivated OoS and SGS repeteadly inoculated i.m. or i.v. into young chiks were similarly immunogenic and elicited a strong immune response detected by the circumsporozoite precipitating (CSP) test performed with living sporozoites. Likewise, a single dose > 10 alive OoS or SGS inoculated i.v. into swiss mice elicited a detectable humoiral anti-sporozoite antibody response measured by CSP tests. Such antibodies were clearly demonstrated only after three weeks of immunization. The titers os specific antibodies measured by CSP or by immunofluorescente against living sporozoites (IFV) were rather low (1:40) with all the immune sera whereas normal sera from chiks or mice reacted with the sporozoite when used undiluted only.The reactivity of both OoS and SGS in vitro was very similar whether in the presence of anti-OoS or anti-SGS sera provide they were fully infective, i.e., when recovered from 10-14 day old infected mosquitos. In this case, no dectable differences were observed in the percentage of reactive sporozoites nor the titer of the reactions. However, the 7-8 day-old poorly infective sporozoites (SGS or OoS) were non-immunogenic and non-reactive in vitro. Our results are the first evidence that avian malaria sporozoites also express the CS proteins, extensively characterized in the mammalian malaria. We are now trying to characterize such proteins, in OoS and SGS at the molecular level in an attempt to elucidate further the similares herein described between both populations and how they compare to other malaria sporozoites. MenosThe infectivity and immunogenicity of two different populations of Plamodium gallinaceum sporozoites and their reactivity in vitro with normal and specific immune sera were studied in paralel experiments. Sporozoites from salivary glands (SGS) or from oocysis (OoS) contained in midguts of Aedes fluviatilis mosquitos gradually acquired infectivity to chiks regardless of their location. This infectivity was abolished after parasite exposure for 30 min to ultraviolete (UV) lights or to X-rays (13Krads). Inactivated OoS and SGS repeteadly inoculated i.m. or i.v. into young chiks were similarly immunogenic and elicited a strong immune response detected by the circumsporozoite precipitating (CSP) test performed with living sporozoites. Likewise, a single dose > 10 alive OoS or SGS inoculated i.v. into swiss mice elicited a detectable humoiral anti-sporozoite antibody response measured by CSP tests. Such antibodies were clearly demonstrated only after three weeks of immunization. The titers os specific antibodies measured by CSP or by immunofluorescente against living sporozoites (IFV) were rather low (1:40) with all the immune sera whereas normal sera from chiks or mice reacted with the sporozoite when used undiluted only.The reactivity of both OoS and SGS in vitro was very similar whether in the presence of anti-OoS or anti-SGS sera provide they were fully infective, i.e., when recovered from 10-14 day old infected mosquitos. In this case, no dectable differences were observed in... Mostrar Tudo |
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Palavras-Chave: |
Imunology. |
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Thesagro: |
Imunologia; Parasitologia. |
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Thesaurus Nal: |
malaria; parasitology; Plasmodium gallinaceum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02717naa a2200229 a 4500
001 1784015
005 2017-04-04
008 1986 bl --- 0-- u #d
100 1 $aKRETTLI, A. U.
245 $aImmunogenicity and infectivity of mature and imature plasmodium gallinaceum sporozoites.
260 $c1986
520 $aThe infectivity and immunogenicity of two different populations of Plamodium gallinaceum sporozoites and their reactivity in vitro with normal and specific immune sera were studied in paralel experiments. Sporozoites from salivary glands (SGS) or from oocysis (OoS) contained in midguts of Aedes fluviatilis mosquitos gradually acquired infectivity to chiks regardless of their location. This infectivity was abolished after parasite exposure for 30 min to ultraviolete (UV) lights or to X-rays (13Krads). Inactivated OoS and SGS repeteadly inoculated i.m. or i.v. into young chiks were similarly immunogenic and elicited a strong immune response detected by the circumsporozoite precipitating (CSP) test performed with living sporozoites. Likewise, a single dose > 10 alive OoS or SGS inoculated i.v. into swiss mice elicited a detectable humoiral anti-sporozoite antibody response measured by CSP tests. Such antibodies were clearly demonstrated only after three weeks of immunization. The titers os specific antibodies measured by CSP or by immunofluorescente against living sporozoites (IFV) were rather low (1:40) with all the immune sera whereas normal sera from chiks or mice reacted with the sporozoite when used undiluted only.The reactivity of both OoS and SGS in vitro was very similar whether in the presence of anti-OoS or anti-SGS sera provide they were fully infective, i.e., when recovered from 10-14 day old infected mosquitos. In this case, no dectable differences were observed in the percentage of reactive sporozoites nor the titer of the reactions. However, the 7-8 day-old poorly infective sporozoites (SGS or OoS) were non-immunogenic and non-reactive in vitro. Our results are the first evidence that avian malaria sporozoites also express the CS proteins, extensively characterized in the mammalian malaria. We are now trying to characterize such proteins, in OoS and SGS at the molecular level in an attempt to elucidate further the similares herein described between both populations and how they compare to other malaria sporozoites.
650 $amalaria
650 $aparasitology
650 $aPlasmodium gallinaceum
650 $aImunologia
650 $aParasitologia
653 $aImunology
700 1 $aLAURENTE, K. E.
700 1 $aDAHER, V. R.
700 1 $aROCHA, E. M. M. da
773 $tMemorias Instituto Owaldo Cruz$gv.81, p.115-122, 1986. Supplement II.
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Registro original: |
Embrapa Pantanal (CPAP) |
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| Registros recuperados : 2 | |
| 1. |  | ALCÂNTARA, J. dos P.; DOURADO, V. V.; ROCHA, E. M. M.; MARQUES, H. S.; NASCIMENTO NETO, J. G.; VASCONCELOS, O. L.; FREIRE FILHO, F. R.; RIBEIRO, V. Q. BAS 202 Rouxinol, novo cultivar de caupi de porte semi-ereto para o Estado da Bahia. Revista Ceres, v. 48, n. 280, p. 723-728, 2001.| Biblioteca(s): Embrapa Meio-Norte. |
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| 2. | | ALCÂNTARA, J. dos P.; DOURADO, V. V.; ROCHA, E. M. M.; MARQUES, H. S.; NASCIMENTO NETO, J. G.; VASCONCELOS, O. L.; FREIRE FILHO, F. R.; RIBEIRO, V. Q. Avaliação de genótipos de caupi de porte enramador e tegumento marrom em diversos ambientes da Bahia. In: REUNIÃO NACIONAL DE PESQUISA DE CAUPI, 5., 2001, Teresina. Avanços tecnológicos no feijão caupi: anais. Teresina: Embrapa Meio-Norte, 2001. p. 149-153 (Embrapa Meio-Norte. Documentos, 56).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio-Norte. |
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| Registros recuperados : 2 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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