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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
22/04/1994 |
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Data da última atualização: |
22/04/1994 |
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Autoria: |
CAMTAMO, M. L.; MORNAN, K.; PLAZAS, J.; ROCA, W. M. |
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Afiliação: |
CIAT, Cali, Colombia. |
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Título: |
Development of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos. |
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Ano de publicação: |
1993 |
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Fonte/Imprenta: |
Cali, Colombia: CIAT, 1993 |
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Páginas: |
185-189p |
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Série: |
CIAT, Working Document, 123 |
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Idioma: |
Inglês |
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Notas: |
International scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedings... Cali, Colombia: CIAT, 1993. |
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Conteúdo: |
Highly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pollen cryopreservation. Conditions were also developed for the isolation, in vitro culture and germination of cassava immature zygotic embryos. The technique was used to recover interspecific Manihot hybrid populations for use in the construction of a cassava molecular linkage map. Culture media and conditions for isolation and culture of pollen and zygotic embryos, and results of work carried out towards the induction of haploidy, will be prosented. |
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Palavras-Chave: |
Congresso. |
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Thesagro: |
Germinação; Inflorescência; Semente. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01521naa a2200241 a 4500
001 1644377
005 1994-04-22
008 1993 bl uuuu u00u1 u #d
100 1 $aCAMTAMO, M. L.
245 $aDevelopment of methodologies for the isolation and culture of cassava immature pollen and zygotic embryos.
260 $c1993
300 $a185-189p
490 $aCIAT, Working Document, 123
500 $aInternational scientific meeting Cassava Biotechnology Network, 1, 1992, Cartajena, Colombia. Proceedings... Cali, Colombia: CIAT, 1993.
520 $aHighly efficient systems for the isolation and in vitro culture of cassava pollen and zygotic embryos have been developed. These methodologies have allowed the manipulation of large quantities of isolated pollen from immature and mature anthers for use in studies towards haploid induction and pollen cryopreservation. Conditions were also developed for the isolation, in vitro culture and germination of cassava immature zygotic embryos. The technique was used to recover interspecific Manihot hybrid populations for use in the construction of a cassava molecular linkage map. Culture media and conditions for isolation and culture of pollen and zygotic embryos, and results of work carried out towards the induction of haploidy, will be prosented.
650 $aGerminação
650 $aInflorescência
650 $aSemente
653 $aCongresso
700 1 $aMORNAN, K.
700 1 $aPLAZAS, J.
700 1 $aROCA, W. M.
773 $tCali, Colombia: CIAT, 1993
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
14/04/2020 |
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Data da última atualização: |
07/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FERREIRA, L. L.; OLIVEIRA FILHO, J. G. de; SILVA, F. de O.; FERRAZ, A. L. L.; MASCARIN, G. M. |
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Afiliação: |
LORENA LOPES FERREIRA, Escola de Veterinária - Belo Horizonte; JAIRES GOMES DE OLIVEIRA FILHO, UFG; FERNANDA DE OLIVEIRA SILVA, UFG; ANA LIVIA LACERDA FERRAZ, Clarion Biociências; GABRIEL MOURA MASCARIN, CNPMA. |
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Título: |
Attract or repel Amblyomma sculptum ticks: screening of semiochemicals. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 278, feb. 2020. Article 109036. |
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Páginas: |
6 p. |
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DOI: |
https://doi.org/10.1016/j.vetpar.2020.109036 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Amblyomma sculptum is a tick of medical-veterinary importance. Areas with high infestations need to be monitored, and parasitizedhosts treated accordingly. Carbon dioxide (CO2) traps and acaricides are commonly deployed as control measures, although with some disadvantages suchas high costs, challenging logistics and vertebrate intoxication. Semiochemicals have the potential to improve tick attraction to traps and monitoring devicesand alleviate the burden of A. sculptum infestations. Four concentrations (10, 5, 2.5 and 1.25 %) of 13 semiochemical candidates (CO2 only at 5 % as thestandard, benzaldehyde, benzoic acid, salicylic acid, 2,6 diclorophenol, R-limonene, S-limonene, methyl salicylate, 1-octen-3-ol, acetone, ammoniumhydroxide, isobutyric acid and lactic acid) were tested on unfed A. sculptum nymphs and adults using a Y-tube olfactometer to evaluated repellence andattraction behaviors. All stages tested were attracted to CO2, whereas nymphs were repelled by benzaldehyde and R-limonene, both at 10 %, and isobutyricacid at 5 and 10 %. Nymphs were attracted by methyl salicylate, benzoic acid and salicylic acid, all at 1.25 %, and by ammonium hydroxide at 2.5 %. Maleswere attracted by benzoic acid at 2.5 %, while females were repelled by benzaldehyde at 5 %. Mixtures with the attractive compounds achieved noattraction response. The compounds that caused attractiveness in the olfactometer assay (CO2, methyl salicylate, benzoic acid, salicylic acid and ammoniumhydroxide) were placed randomly in traps in a grassland plot naturally infested with A. sculptum in triplicate. Notably, dry ice (CO2) remained the best atluring ticks in the field (P < 0.001). Benzoic acid should be further investigated since attractant activity was strongly confirmed in both laboratory and field tests. On the other hand, isobutyric acid and R-limonene could be better exploited due to their repellent role revealed by the lab assay, which makes themworthwhile molecules as natural repellents for the management of this tick. MenosAbstract: Amblyomma sculptum is a tick of medical-veterinary importance. Areas with high infestations need to be monitored, and parasitizedhosts treated accordingly. Carbon dioxide (CO2) traps and acaricides are commonly deployed as control measures, although with some disadvantages suchas high costs, challenging logistics and vertebrate intoxication. Semiochemicals have the potential to improve tick attraction to traps and monitoring devicesand alleviate the burden of A. sculptum infestations. Four concentrations (10, 5, 2.5 and 1.25 %) of 13 semiochemical candidates (CO2 only at 5 % as thestandard, benzaldehyde, benzoic acid, salicylic acid, 2,6 diclorophenol, R-limonene, S-limonene, methyl salicylate, 1-octen-3-ol, acetone, ammoniumhydroxide, isobutyric acid and lactic acid) were tested on unfed A. sculptum nymphs and adults using a Y-tube olfactometer to evaluated repellence andattraction behaviors. All stages tested were attracted to CO2, whereas nymphs were repelled by benzaldehyde and R-limonene, both at 10 %, and isobutyricacid at 5 and 10 %. Nymphs were attracted by methyl salicylate, benzoic acid and salicylic acid, all at 1.25 %, and by ammonium hydroxide at 2.5 %. Maleswere attracted by benzoic acid at 2.5 %, while females were repelled by benzaldehyde at 5 %. Mixtures with the attractive compounds achieved noattraction response. The compounds that caused attractiveness in the olfactometer assay (CO2, methyl salicylate, benzoic acid, salicylic acid and ammoniumhy... Mostrar Tudo |
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Palavras-Chave: |
Alternative tick control; Amblyomma cajennense sensu lato; Field trial; Y-tube olfactometer. |
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Thesagro: |
Atrativo; Carrapato; Repelente. |
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Thesaurus NAL: |
Insect attractants; Insect repellents; Olfactometers; Semiochemicals; Tick control. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 03037naa a2200337 a 4500
001 2121636
005 2023-02-07
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetpar.2020.109036$2DOI
100 1 $aFERREIRA, L. L.
245 $aAttract or repel Amblyomma sculptum ticks$bscreening of semiochemicals.$h[electronic resource]
260 $c2020
300 $a6 p.
520 $aAbstract: Amblyomma sculptum is a tick of medical-veterinary importance. Areas with high infestations need to be monitored, and parasitizedhosts treated accordingly. Carbon dioxide (CO2) traps and acaricides are commonly deployed as control measures, although with some disadvantages suchas high costs, challenging logistics and vertebrate intoxication. Semiochemicals have the potential to improve tick attraction to traps and monitoring devicesand alleviate the burden of A. sculptum infestations. Four concentrations (10, 5, 2.5 and 1.25 %) of 13 semiochemical candidates (CO2 only at 5 % as thestandard, benzaldehyde, benzoic acid, salicylic acid, 2,6 diclorophenol, R-limonene, S-limonene, methyl salicylate, 1-octen-3-ol, acetone, ammoniumhydroxide, isobutyric acid and lactic acid) were tested on unfed A. sculptum nymphs and adults using a Y-tube olfactometer to evaluated repellence andattraction behaviors. All stages tested were attracted to CO2, whereas nymphs were repelled by benzaldehyde and R-limonene, both at 10 %, and isobutyricacid at 5 and 10 %. Nymphs were attracted by methyl salicylate, benzoic acid and salicylic acid, all at 1.25 %, and by ammonium hydroxide at 2.5 %. Maleswere attracted by benzoic acid at 2.5 %, while females were repelled by benzaldehyde at 5 %. Mixtures with the attractive compounds achieved noattraction response. The compounds that caused attractiveness in the olfactometer assay (CO2, methyl salicylate, benzoic acid, salicylic acid and ammoniumhydroxide) were placed randomly in traps in a grassland plot naturally infested with A. sculptum in triplicate. Notably, dry ice (CO2) remained the best atluring ticks in the field (P < 0.001). Benzoic acid should be further investigated since attractant activity was strongly confirmed in both laboratory and field tests. On the other hand, isobutyric acid and R-limonene could be better exploited due to their repellent role revealed by the lab assay, which makes themworthwhile molecules as natural repellents for the management of this tick.
650 $aInsect attractants
650 $aInsect repellents
650 $aOlfactometers
650 $aSemiochemicals
650 $aTick control
650 $aAtrativo
650 $aCarrapato
650 $aRepelente
653 $aAlternative tick control
653 $aAmblyomma cajennense sensu lato
653 $aField trial
653 $aY-tube olfactometer
700 1 $aOLIVEIRA FILHO, J. G. de
700 1 $aSILVA, F. de O.
700 1 $aFERRAZ, A. L. L.
700 1 $aMASCARIN, G. M.
773 $tVeterinary Parasitology$gv. 278, feb. 2020. Article 109036.
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Embrapa Meio Ambiente (CNPMA) |
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