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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
07/04/2004 |
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Data da última atualização: |
07/04/2004 |
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Autoria: |
ROA, A. C.; CHAVARRIAGA, P.; DUQUE, M. C.; BONIERBALE, M. W.; THOME, J.; GARY KOCHERT, C. |
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Título: |
Microsatellites as a tool for assessing genetic diversity in Manihot species. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p.17, nov, 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Cassava and its wild relatives have been studied under different approaches, but there are still doubts about the possible ancestors of the crop and the level of variation present in the cultivated and the wild germplasm. Microsatellites or Simples Sequence Repeats (SSRS) have been widely recognized as powerful and informative genetic markers in plants and their utility results from their inherent variability even within populations. In order to evaluate the informativeness of these codominant markers in Manihot, a set of wild species that have been proposed as close relatives and a representative sample of the crop were assessed with 10 different primers pairs developed from cassava. In a samdle of 121 individuals were detected 124 alleles. From these, 79 were only in the wild taxa, indicating a greater alielic diversity than in the cultivated species. The numbre of alleles per locus ranged from 4 to 21 and the percentage of polynorphic loci varied from 57 up to 100. In general terms, microsatellite primers worked across the genus, but with increasing genetic distance there was a clear tendency for decreasing ability to successful amplify loci. Manihot aesculifolia, M. brachloba and M. carthaginensis did not present a banding paftern at 2 of the loci evaluted. A previous work with AFLP markers and the present STMS analysis showed that these 3 wild taxa are the most distant relatives to the crop, while the wild forms M. esculenta subsp. flabellifolia and M. esculenta subsp. peruviana appeared to be the most similar to Cassava. At the different loci, unique alleles were present mainly at the wild species. Microsatellite markers were particularly useful for showing a finescale differentiation within species. MenosAbstract: Cassava and its wild relatives have been studied under different approaches, but there are still doubts about the possible ancestors of the crop and the level of variation present in the cultivated and the wild germplasm. Microsatellites or Simples Sequence Repeats (SSRS) have been widely recognized as powerful and informative genetic markers in plants and their utility results from their inherent variability even within populations. In order to evaluate the informativeness of these codominant markers in Manihot, a set of wild species that have been proposed as close relatives and a representative sample of the crop were assessed with 10 different primers pairs developed from cassava. In a samdle of 121 individuals were detected 124 alleles. From these, 79 were only in the wild taxa, indicating a greater alielic diversity than in the cultivated species. The numbre of alleles per locus ranged from 4 to 21 and the percentage of polynorphic loci varied from 57 up to 100. In general terms, microsatellite primers worked across the genus, but with increasing genetic distance there was a clear tendency for decreasing ability to successful amplify loci. Manihot aesculifolia, M. brachloba and M. carthaginensis did not present a banding paftern at 2 of the loci evaluted. A previous work with AFLP markers and the present STMS analysis showed that these 3 wild taxa are the most distant relatives to the crop, while the wild forms M. esculenta subsp. flabellifolia and M. esculen... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02280naa a2200181 a 4500
001 1651860
005 2004-04-07
008 1998 bl uuuu u00u1 u #d
100 1 $aROA, A. C.
245 $aMicrosatellites as a tool for assessing genetic diversity in Manihot species.
260 $c1998
520 $aAbstract: Cassava and its wild relatives have been studied under different approaches, but there are still doubts about the possible ancestors of the crop and the level of variation present in the cultivated and the wild germplasm. Microsatellites or Simples Sequence Repeats (SSRS) have been widely recognized as powerful and informative genetic markers in plants and their utility results from their inherent variability even within populations. In order to evaluate the informativeness of these codominant markers in Manihot, a set of wild species that have been proposed as close relatives and a representative sample of the crop were assessed with 10 different primers pairs developed from cassava. In a samdle of 121 individuals were detected 124 alleles. From these, 79 were only in the wild taxa, indicating a greater alielic diversity than in the cultivated species. The numbre of alleles per locus ranged from 4 to 21 and the percentage of polynorphic loci varied from 57 up to 100. In general terms, microsatellite primers worked across the genus, but with increasing genetic distance there was a clear tendency for decreasing ability to successful amplify loci. Manihot aesculifolia, M. brachloba and M. carthaginensis did not present a banding paftern at 2 of the loci evaluted. A previous work with AFLP markers and the present STMS analysis showed that these 3 wild taxa are the most distant relatives to the crop, while the wild forms M. esculenta subsp. flabellifolia and M. esculenta subsp. peruviana appeared to be the most similar to Cassava. At the different loci, unique alleles were present mainly at the wild species. Microsatellite markers were particularly useful for showing a finescale differentiation within species.
700 1 $aCHAVARRIAGA, P.
700 1 $aDUQUE, M. C.
700 1 $aBONIERBALE, M. W.
700 1 $aTHOME, J.
700 1 $aGARY KOCHERT, C.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p.17, nov, 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 2 | |
| 1. |  | ALLEM, A. C.; ROA, A. C.; MENDES, R. A.; SALOMÃO, A. N.; BURLE, M. L.; SECOND, G.; CARVALHO, P. C. L. de; CAVALCANTI, J. The primary genepool of cassava (Manihot esculenta Crantz). Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 11, 1998. Suplemento. Edição dos Resumos do IV International Scientific Meeting of the Cassava Biotechnology Network, Salvador,1998.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 2. | | ALLEM, A. C.; ROA, A. C.; MENDES, R. A.; SALOMAO, A. N.; BURLE, M. L.; SECOND, G.; CARVALHO, P. C. L. de; CAVALCANTI, J. The primary gene pool of cassava (Manihot esculenta Crantz). In: INTERNATIONAL SCIENTIFIC MEETING CASSAVA BIOTECHNOLOGY NETWORK, 4., 1998, Salvador. Cassava biotechnology: proceedings. Brasília, DF: EMBRAPA-CENARGEN: CBN, 2000. P. 3-14.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| Registros recuperados : 2 | |
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