Data/hora: 03/06/2024 18:30:03

Biblioteca(s): | Embrapa Florestas.
Data corrente: | 09/10/2008
Data da última atualização: | 09/10/2008
Autoria: | RO¿EN, A.; SOBCZYK, £.; WEINER, J.
Título: | The effect of preservation methods upon the results of stoichiometric measurements in invertebrates.
Ano de publicação: | 2008
Fonte/Imprenta: | In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
Idioma: | Inglês

Conteúdo:

The growing interest in applying stoichiometry approach to community ecology resulted in
numerous studies concerning aquatic systems, while terrestrial (soil) biota are still poorly known
in this respect. Ecological stoichiometry requires analyzing body content of several elements in
numerous individuals, particularly if small-bodied organisms such as soil invertebrates are
studied, which requires an extensive sampling in the field. On the other hand, soil fauna is often
sampled for other purposes, such as biodiversity or population ecology studies. The question
arises whether such a rich material could also be used for stoichiometric studies, if body
composition would not change depending on conservation technique used. Invertebrates collected
in the field could be preserved in different ways, probably most commonly in formaldehyde or in
ethanol. Animals captured by pitfall-traps are often put initially into formaldehyde and later
transferred into ethanol. The aim of present study was to check how different conservation
methods influence concentration of Fe, Ca, Mg, Mn, K, Zn, and Cu. To check that, a selection of
individuals of different taxons (earthworms of two sizes (Eisenia fetida), cricets (Gryllus similis),
tenebrionid larvae (Tenebrio molitor), Diptera larvae (Musca domestica), cockroaches
(Gromphadorhina portentosa) and wood boring beetles larvae (Zoophobas morio)), all from
commercial cultures, were preserved using following methods: (1) frozen, (2) kept for 4 weeks
in formaldehyde; (3) kept for 2 weeks in formaldehyde and then for 2 weeks in ethanol. The
contents of Mg, K, Ca, Fe, Cu, Mn, Zn was measured by AAS using standard procedures.
Differences have been found in elements content depending on the element, preservation
method, taxon, and body size. Earthworms contained significantly more Fe and Ca than other
invertebrates studied, whereas tenebrionid larvae characterised higher concentration of Mg.
The results show significant effect of preservation method on K concentration (frozen animals
contained up to 200 times more K than those preserved by either method). Also the measured
content of Mn and Mg was influenced by preservation method while no such effect was detected
for Fe, Ca, Cu and Zn.

Categoria do assunto: | --
Registro original: | Embrapa Florestas (CNPF)
Exemplares: | CNPF - (43875-1) - UPE - PL - -- - CD0305 -  -  - DIS