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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
08/08/2007 |
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Data da última atualização: |
25/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
GIMÉNEZ-PECCI, M. P.; CONCI, L. R.; TRUOL, G.; NAGATA, T.; KANEMATSU, S.; LAGUNA, I. G.; OLIVEIRA, E.; RESENDE, R. O. |
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Afiliação: |
M. P. Giménez_Peddi, INTA; L. R. Conci, INTA; G. Truol, INTA; T. Nagata, Universidade Católica de Brasília; S. Kanematsu, National Agricultural Research Center for Tohoku Region; I. G. Laguna, INTA; ELIZABETH DE OLIVEIRA SABATO, CNPMS; R. O. Resende, Universidade de Brasília. |
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Título: |
Molecular diversity of ecologically distinct Mal de Rio Cuarto virus isolates based on restriction fragment length polymorphism (RFLPs) and genome sequence analysis of segments 1, 7, 9 and 10. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Archives of Virology, New York, v. 152, n. 7, p. 1341-1351, Jul. 2007. |
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DOI: |
10.1007/s00705-007-0944-y |
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Idioma: |
Inglês |
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Conteúdo: |
Viruses of the species Mal de Río Cuarto virus (genus Fijivirus, family Reoviridae) cause significant economic losses in maize in Argentina. Genetic changes in the virus genome leading to better adaptation to diverse ecological conditions were postulated that would account for the increasing MRCV variability. The genomic differences between MRCV isolates from four ecologically different areas (Río Cuarto, RC; Pergamino, P; Jesús María, JM; and Tafí del Valle, TV) were studied. RT-PCR-amplified fragments comprising four genomic segments (Seg1, Seg7, Seg9 and Seg10) of MRCV isolates were compared by RFLPs and nucleotide sequences. The segments were chosen based on the proteins they encode: RNA-dependent-RNA polymerase, proteins putatively associated with tubular structures and viroplasm and the major outer capsid protein, respectively. Genetic comparison suggested that JM and TV isolates were genetically similar, but RC and P were different. Therefore, they were clustered in three genetic groups (JM = TV, RC and P). Together, nucleotide and amino acid sequence identities of the genomic segments were often above 96%. Seg1 was more variable (viral polymerase), whereas Seg7 (putative tubular structure) was the most conserved. Phylogeny analysis showed that MRCV isolates could be clustered in ?mountain area? and ?high production area? groups according to their geographical occurrence. |
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Thesagro: |
Vírus. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02195naa a2200229 a 4500
001 1490363
005 2018-05-25
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1007/s00705-007-0944-y$2DOI
100 1 $aGIMÉNEZ-PECCI, M. P.
245 $aMolecular diversity of ecologically distinct Mal de Rio Cuarto virus isolates based on restriction fragment length polymorphism (RFLPs) and genome sequence analysis of segments 1, 7, 9 and 10.$h[electronic resource]
260 $c2007
520 $aViruses of the species Mal de Río Cuarto virus (genus Fijivirus, family Reoviridae) cause significant economic losses in maize in Argentina. Genetic changes in the virus genome leading to better adaptation to diverse ecological conditions were postulated that would account for the increasing MRCV variability. The genomic differences between MRCV isolates from four ecologically different areas (Río Cuarto, RC; Pergamino, P; Jesús María, JM; and Tafí del Valle, TV) were studied. RT-PCR-amplified fragments comprising four genomic segments (Seg1, Seg7, Seg9 and Seg10) of MRCV isolates were compared by RFLPs and nucleotide sequences. The segments were chosen based on the proteins they encode: RNA-dependent-RNA polymerase, proteins putatively associated with tubular structures and viroplasm and the major outer capsid protein, respectively. Genetic comparison suggested that JM and TV isolates were genetically similar, but RC and P were different. Therefore, they were clustered in three genetic groups (JM = TV, RC and P). Together, nucleotide and amino acid sequence identities of the genomic segments were often above 96%. Seg1 was more variable (viral polymerase), whereas Seg7 (putative tubular structure) was the most conserved. Phylogeny analysis showed that MRCV isolates could be clustered in ?mountain area? and ?high production area? groups according to their geographical occurrence.
650 $aVírus
700 1 $aCONCI, L. R.
700 1 $aTRUOL, G.
700 1 $aNAGATA, T.
700 1 $aKANEMATSU, S.
700 1 $aLAGUNA, I. G.
700 1 $aOLIVEIRA, E.
700 1 $aRESENDE, R. O.
773 $tArchives of Virology, New York$gv. 152, n. 7, p. 1341-1351, Jul. 2007.
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