Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
09/10/2008 |
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Data da última atualização: |
02/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, L. K.; MELO, L. C.; BRONDANI, C.; DEL PELOSO, M. J.; BRONDANI, R. P. V. |
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Afiliação: |
L. K. OLIVEIRA, Universidade Católica de Goiás; LEONARDO CUNHA MELO, CNPAF; CLAUDIO BRONDANI, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Backcross assisted by microsatellite markers in common bean. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 7, n. 4, p. 1000-1010, 2008. |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virusand, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. For crosses ARC100-4 x DOR303 and ARC100-4 x PHAS8328, assisted selection was carried out in the F2 and F2BC1generations, while in the remaining crosses selection was performed only in the F2BC1 generation. For the microsatellite analysis, in each generation, 20 markers were genotyped. The molecular data were analyzed using the NTSys program and the proportion of the recurrent genome introgressed was estimated, based on genotypical configuration of the segregant populations compared to the recurrent parents. The results indicate a higher efficiency in recovering the genotype of the elite genitor through the strategy of backcross assisted selection in the successive generations, and demonstrate a practical and useful application of molecular marker technology associated with bean breeding, to reduce the number of backcrosses and the time to recover the genome of the recurrent genitors. |
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Palavras-Chave: |
Genetic breeding; Molecular markers. |
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Thesagro: |
Feijão; Melhoramento Genético Vegetal; Phaseolus Vulgaris. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224578/1/gmr-2008.pdf
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Marc: |
LEADER 01934naa a2200229 a 4500
001 1217398
005 2022-06-02
008 2008 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, L. K.
245 $aBackcross assisted by microsatellite markers in common bean.$h[electronic resource]
260 $c2008
520 $aThe objectives of the present study were to monitor the effect of backcrossing through microsatellite markers and to compare different marker assisted selection strategies. Four populations were developed using donor parents resistant to the bean golden mosaic virusand, for all crosses, only individuals resistant to the bean golden mosaic virus were backcrossed. For crosses ARC100-4 x DOR303 and ARC100-4 x PHAS8328, assisted selection was carried out in the F2 and F2BC1generations, while in the remaining crosses selection was performed only in the F2BC1 generation. For the microsatellite analysis, in each generation, 20 markers were genotyped. The molecular data were analyzed using the NTSys program and the proportion of the recurrent genome introgressed was estimated, based on genotypical configuration of the segregant populations compared to the recurrent parents. The results indicate a higher efficiency in recovering the genotype of the elite genitor through the strategy of backcross assisted selection in the successive generations, and demonstrate a practical and useful application of molecular marker technology associated with bean breeding, to reduce the number of backcrosses and the time to recover the genome of the recurrent genitors.
650 $aFeijão
650 $aMelhoramento Genético Vegetal
650 $aPhaseolus Vulgaris
653 $aGenetic breeding
653 $aMolecular markers
700 1 $aMELO, L. C.
700 1 $aBRONDANI, C.
700 1 $aDEL PELOSO, M. J.
700 1 $aBRONDANI, R. P. V.
773 $tGenetics and Molecular Research$gv. 7, n. 4, p. 1000-1010, 2008.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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