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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Rondônia. |
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Data corrente: |
08/02/2017 |
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Data da última atualização: |
10/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOS, F. T. de; RINCON, J. A. A.; ACOSTA, D. A. V.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORRÊA, M. N.; GASPERIN, B. G.; PFEIFER, L. F. M.; BARROS, C. C.; PEGORARO, L. M. C.; SCHNEIDER, A. |
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Afiliação: |
Felipe Terres de Campos, UFPel; Joao Aveiro Alvarado Rincon, UFPel; Diego Andres Velasco Acosta, CORPOICA; Pedro Augusto Silva Silveira, UFPel; Jorgea Pradieé, Embrapa Clima Temperado; Marcio Nunes Corrêa, UFPel; Bernardo Garziera Gasperin, UFPel; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-Rondonia; Carlos Castilho Barros, UFPel; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; Augusto Schneider, UFPel. |
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Título: |
The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Theriogenology, v. 89, p. 244 - 249, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells. MenosThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic a... Mostrar Tudo |
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Palavras-Chave: |
Esteroidogênese; PON1. |
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Thesagro: |
Colesterol; Inflamação. |
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Thesaurus Nal: |
cholesterol; inflammation; steroidogenesis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227597/1/cpafro-17770.pdf
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Marc: |
LEADER 02572naa a2200325 a 4500
001 2063230
005 2021-11-10
008 2017 bl uuuu u00u1 u #d
100 1 $aCAMPOS, F. T. de
245 $aThe acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.$h[electronic resource]
260 $c2017
520 $aThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.
650 $acholesterol
650 $ainflammation
650 $asteroidogenesis
650 $aColesterol
650 $aInflamação
653 $aEsteroidogênese
653 $aPON1
700 1 $aRINCON, J. A. A.
700 1 $aACOSTA, D. A. V.
700 1 $aSILVEIRA, P. A. S.
700 1 $aPRADIEÉ, J.
700 1 $aCORRÊA, M. N.
700 1 $aGASPERIN, B. G.
700 1 $aPFEIFER, L. F. M.
700 1 $aBARROS, C. C.
700 1 $aPEGORARO, L. M. C.
700 1 $aSCHNEIDER, A.
773 $tTheriogenology$gv. 89, p. 244 - 249, 2017.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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| Registros recuperados : 27 | |
| 21. |  | CAMPOS, F. T. de; RINCON, J. A. A.; SILVA, J. F. da; VIEIRA, A. D.; MION, B.; MEDEIRA, E. M.; PRIETSCH, L.; GOUVEIA, G. V.; PEGORARO, L. M. C.; SCHNEIDER, A. Concentrações de paraoxonase 1 (PON1) no soro de ovelhas crioulas lanadas avaliados durante o ciclo reprodutivo. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 22. | | RINCÓN, J. A. A.; MADEIRA, E. M.; CAMPOS, F. T.; MION, B.; SILVA, J. F.; ABSALÓN-MEDINA, V. A.; BUTLER, W. R.; CORRÊA, M. N.; PEGORARO, L. M. C.; SCHNEIDER, A. Exogenous paraoxonase-1 during oocyte maturation improves bovine embryo development in vitro. Reproduction in Domestic Animals, p. 1-4, 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 23. | | COLLARES, L. F.; RINCÓN, J. A. A.; MION, B.; GINDRI, P. C.; BORGES, M. A.; SILVA, J. F. DA; PRADIEÉ, J.; PEGORARO, L. M. C.; SCHNEIDER, A.; CORREA, M. N. Does the addition of cow serum during the recent post partum period influence the initial embryonic development? In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Publicado na revista Animal Reproduction, v. 13, n. 3, p. 598, Jul./Sept. 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 24. | | RINCÓN, J. A. A.; GINDRI, P. C.; MION, B.; FERRONATO, G. de A.; BARBOSA, A. A.; MAFFI, A. S.; PRADIEÉ, J.; MONDADORI, R. G.; CORRÊA, M. N.; PEGORARO, L. M. C.; SCHNEIDER, A. Early embryonic development of bovine oocytes challenged with LPS in vitro or in vivo. Reproduction, v. 158, p. 453?463, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 25. | | GINDRI, P.; CASTRO, N. de A.; MION, B.; GASPERIN, B. G.; PEGORARO, L. M. C.; RINCÓN, J. A. A.; VEIRIRA, A. D.; PRADIEÉ, J.; PFEIFER, L. F. M.; CORRÊA, M. N.; SCHNEIDER, A. Intrafollicular lipopolysaccharide injection delays ovulation in cows. Animal Reproduction Science, v. 211, p. 1-8, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Rondônia. |
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| 26. | | HAX, L. T.; RINCÓN, J. A. A.; SCHNEIDER, A.; PEGORARO, L. M. C.; COLLARES, L. F.; PEREIRA, R. A.; PRADIEÉ, J.; DEL PINO, F. A. B.; CORRÊA, M. N. Effect of butafosfan supplementation during oocyte maturation on bovine embryo development. ZYGOTE (CAMBRIDGE. PRINT), 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 27. | | RINCON, J. A. A.; PRADIEÉ, J.; FEIJÓ, J. de O.; REMIÃO, M. H.; BORGES, M. A.; LPES, I. A. R.; CORREA, M. N.; PEGORARO, L. M. C.; SCHNEIDER, A. Effect of high density lipoprotein during in vitro oocyte maturation on initial embryo development in bovine. Reproduction in Domestic Animals, v. 14, n. 3, p. 749, jul/sept, 2017. Publicado: Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo
Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 27 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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