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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
04/07/2019 |
Data da última atualização: |
04/07/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
CAITANO-BERTOLACCI, M. A. B.; BASTOS, R. R. P.; SANTOS, L. R. dos; SOARES, C. O.; RIEGER, J. S. G.; MANTOVANI, C.; SANTANA, M. de S.; ROSINHA, G. M. S. |
Afiliação: |
Universidade Federal de Mato Grosso do Sul/FUFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia; LENITA RAMIRES DOS SANTOS, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
Título: |
DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 2, gmr18295, 2019 |
Idioma: |
Inglês |
Conteúdo: |
VIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection. MenosVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellu... Mostrar Tudo |
Palavras-Chave: |
DNA vaccine; T4SS; VirB operon; VirB12; VirB9. |
Thesagro: |
Brucella Abortus. |
Thesaurus Nal: |
Bovine brucellosis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02847naa a2200289 a 4500 001 2110376 005 2019-07-04 008 2019 bl uuuu u00u1 u #d 100 1 $aCAITANO-BERTOLACCI, M. A. B. 245 $aDNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice.$h[electronic resource] 260 $c2019 520 $aVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection. 650 $aBovine brucellosis 650 $aBrucella Abortus 653 $aDNA vaccine 653 $aT4SS 653 $aVirB operon 653 $aVirB12 653 $aVirB9 700 1 $aBASTOS, R. R. P. 700 1 $aSANTOS, L. R. dos 700 1 $aSOARES, C. O. 700 1 $aRIEGER, J. S. G. 700 1 $aMANTOVANI, C. 700 1 $aSANTANA, M. de S. 700 1 $aROSINHA, G. M. S. 773 $tGenetics and Molecular Research$gv. 18, n. 2, gmr18295, 2019
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Registros recuperados : 87 | |
2. | | CARDOSO, M. S.; MARCELINO, F. C.; BARROS, E. G. de. Validação Ddos genes da W-gliadina e da glutenina como referências endógenas para a detecção e quantificação e resíduos de transgênicos em trigo (Triticum aestivum). In: CONGRESSO BRASILEIRO DE BIOSSEGURANÇA, 5.; SIMPÓSIO LATINO AMERICANO DE PRODUTOS TRANSGÊNICOS, 5.; SEMINÁRIO DE BIOENERGIA, 1.; SIMPÓSIO DE POPULARIZAÇÃO DA BIOTECNOLOGIA, 2.; MOSTRA DE BIOTECNOLOGIA PARA A SOCIEDADE, 1., 2007, Ouro Preto, MG. Anais dos eventos... Rio de Janeiro: Associação Nacional de Biossegurança, 2007. p.121.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Soja. |
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5. | | PINTO, M. de O.; GOOD-GOD, P. I. V.; MOREIRA, M. A.; BARROS, E. G. de. Associação de marcadores moleculares SNP com o conteúdo de ácido linolênico em sementes de soja. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 3, p. 263-269, mar. 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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6. | | FALEIRO, F. G.; CRUZ, C. D.; CASTRO, C. de; MOREIRA, M. A.; BARROS, E. G. de. Comparação de blocos casualizados e testemunhas intercalares na estimação de parâmetros genéticos em feijoeiro Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 12, p. 1675-1680, dez. 2002 Título em inglês: Matching of random blocks and intercalated checks for genetic parameters estimation in common bean.Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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10. | | SCHUSTER, I.; QUEIROZ, V. T. de; TEIXEIRA, A. I.; BARROS, E. G. de; MOREIRA, M. A. Determinação da pureza varietal de sementes de soja com o auxílio de marcadores moleculares microssatélites. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 39, n. 3, p. 247-253, mar. 2004 Título em inglês: Determination of genetic purity of soybean seeds with the aid of microsatellite molecular markers.Biblioteca(s): Embrapa Unidades Centrais. |
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12. | | MARCELINO, F. C.; BARROS, E. G. de; GUIMARÃES, M. F. C. Detection and quantificiation of Roundup Ready soybean residues in sausage samples by conventional and real-time PCR. In: CONGRESSO BRASILEIRO DE BIOSSEGURANÇA, 5.; SIMPÓSIO LATINO AMERICANO DE PRODUTOS TRANSGÊNICOS, 5.; SEMINÁRIO DE BIOENERGIA, 1.; SIMPÓSIO DE POPULARIZAÇÃO DA BIOTECNOLOGIA, 2.; MOSTRA DE BIOTECNOLOGIA PARA A SOCIEDADE, 1., 2007, Ouro Preto, MG. Anais dos eventos... Rio de Janeiro: Associação Nacional de Biossegurança, 2007. p. 122.Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
Biblioteca(s): Embrapa Soja. |
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15. | | CERVIGNI, G. D. L.; SCHUSTER, I.; SEDIYAMA, C. S.; BARROS, E. G. de; MOREIRA, M. A. Inherit ance pattern and selection criteria for resistance to soybean cyst nematode races 3 and 9. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 10, p. 1413-1419, out. 2007 Título em português: Padrão de herança e critérios de seleção para resistênciaàs raças 3 e 9 do nematóide-de-cisto-da-soja.Biblioteca(s): Embrapa Unidades Centrais. |
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16. | | RIBEIRO, C. A. G.; TANURE, J. P. M.; MACIEL, T. E. F.; BARROS, E. G. de. Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 3, p. 270-279, mar. 2013. Título em português: Caracterização molecular de cultivares de soja por meio de marcadores microssatélites com sequência de cauda universal.Biblioteca(s): Embrapa Unidades Centrais. |
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17. | | SOUZA, T. L. P. O.; ALZATE-MARIN, A. L.; FALEIRO, F. G.; BARROS, E. G. de. Pathosystem common bean-uromyces appendiculatus: host resistance, pathogen specialization, and breeding for rust resistance. Pest Technology, v. 2, n. 2, p. 56-69, 2008.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
Biblioteca(s): Embrapa Cerrados. |
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20. | | VILARINHOS, A. D.; BARROS, E. G. de; PAIVA, E.; SEDIYAMA, C. S.; MOREIRA, M. A. Use of the random amplified polymorphic DNA technique to characterize soybean (Glycine max (L.) Merrill) genotypes. Revista Brasileira de Genética, Ribeirão Preto, v. 17, n. 3, p. 287-290, 1994.Tipo: Artigo em Periódico Indexado |
Biblioteca(s): Embrapa Milho e Sorgo. |
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Registros recuperados : 87 | |
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