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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/09/2020 |
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Data da última atualização: |
16/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NERY, F. M. B.; MELO, F. L.; BOITEUX, L. S.; RIBEIRO, S. da G.; RESENDE, R. O.; ORÍLIO, A. F.; BATISTA, J. G.; LIMA, M. F.; PEREIRA-CARVALHO, R. C. |
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Afiliação: |
FLÁVIA M. B. NERY, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; LEONARDO SILVA BOITEUX, CNPH; SIMONE DA GRACA RIBEIRO, Cenargen; RENATO O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANELISE F. ORÍLIO, UNIVERSIDADE DE BRASÍLIA; JOSIANE G. BATISTA, UNIVERSIDADE DE BRASÍLIA; MIRTES FREITAS LIMA, CNPH; RITA C. PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Molecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the Order Picornavirales. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Viruses, v. 12, n. 9, 950, 2020 |
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DOI: |
https://doi.org/10.3390/v12090950 |
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Idioma: |
Inglês |
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Conteúdo: |
In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered. MenosIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel viru... Mostrar Tudo |
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Palavras-Chave: |
HDaV1; HDaV2; Virome. |
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Thesagro: |
Hovenia Dulcis. |
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Thesaurus Nal: |
Metagenomics; Picornavirales; Viruses. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219297/1/ARTIGO-MolecularCharacterizationHoveniaDulcis.pdf
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Marc: |
LEADER 02445naa a2200313 a 4500
001 2128264
005 2020-12-16
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/v12090950$2DOI
100 1 $aNERY, F. M. B.
245 $aMolecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2)$bnew tentative species within the Order Picornavirales.$h[electronic resource]
260 $c2020
520 $aIn a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.
650 $aMetagenomics
650 $aPicornavirales
650 $aViruses
650 $aHovenia Dulcis
653 $aHDaV1
653 $aHDaV2
653 $aVirome
700 1 $aMELO, F. L.
700 1 $aBOITEUX, L. S.
700 1 $aRIBEIRO, S. da G.
700 1 $aRESENDE, R. O.
700 1 $aORÍLIO, A. F.
700 1 $aBATISTA, J. G.
700 1 $aLIMA, M. F.
700 1 $aPEREIRA-CARVALHO, R. C.
773 $tViruses$gv. 12, n. 9, 950, 2020
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Registro original: |
Embrapa Hortaliças (CNPH) |
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| Registros recuperados : 99 | |
| 8. |  | RIBEIRO, S. da G.; ARAUJO, A. C. G. Preparação de sondas. In: BRASILEIRO, A. C. M.; CARNEIRO, V. T. de C. (Ed.). Manual de transformação genética de plantas. 2.ed. rev. e ampl. Brasília, DF: Embrapa, 2015. p. 199-1 213.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | GIORDANO, L. de B.; BEZERRA, I. C.; RIBEIRO, S. da G.; AVILA, A. C. de. Breeding tomatoes (Lycopersicon esculentum Mill.) for resistance to whitefly-transmitted geminiviruses. In: INTERNATIONAL CONFERENCE ON THE PROCESSING TOMATO, 1., INTERNATIONAL SYMPOSIUM ON TROPICAL TOMATO DISEASES, 1., 1996, Recife. Proceedings... Alexandria: ASHS / IPA, 1997. p.150-151.| Biblioteca(s): Embrapa Hortaliças. |
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| 12. | | LIMA, M. F.; RIBEIRO, S. da G.; NAGATA, A. K. I.; NAKASU, E. Y. T. Identification of a potatoinfecting begomovirus in the central region of Brazil. Virus Reviews and Research, v. 20, n. 2, p. 145, 2016. Edição dos resumos do XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016. Resumo PIV239.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | LIMA, M. F.; RIBEIRO, S. da G.; NAGATA, A. K. I.; NAKASU, E. Y. T. Identification of a potatoinfecting begomovirus in the central region of Brazil. Virus Reviews and Research, v. 20, n. 2, p. 145, 2016. Edição dos resumos do XXVII Brazilian Congress of Virology & XI Mercosur Meeting of Virology, Pirienópolis, GO, 2016. Na publicação: Inoue Nagata, A. K. Resumo PIV239.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
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| 15. | | LIMA, M. F.; BEZERRA, I. C.; RIBEIRO, S. da G.; AVILA, A. C. de. Levantamento de geminivírus na cultura do tomate no Submédio do Vale do São Francisco. Fitopatologia Brasleira, Brasília, DF, v. 23, p. 319, ago. 1998. Suplemento. Edição dos Resumos do 31 Congresso Brasileiro de Fitopatologia, Fortaleza, 1998.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 20. | | NOGUEIRA, M. P.; RIBEIRO, S. da G.; FONTENELE, R. S.; LACORTE, C. C.; MEHTA, A. Proteômica de folhas de tomate (Solanum lycopersicum) inoculadas com Tomato chlorotic motte vírus (toCMoV). In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia, SP. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 69.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 99 | |
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