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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
02/02/2017 |
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Data da última atualização: |
19/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DELAMUTA, J. R. M.; RIBEIRO, R. A.; ARAUJO, J. L. S. de; ROUWS, L. F. M.; ZILLI, J. E.; PARMA, M. M.; MELO, I. S. de; HUNGRIA, M. |
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Afiliação: |
JAKELINE RENATA MARCON DELAMUTA, CAPES; RENAN AUGUSTO RIBEIRO, CNPq; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; LUC FELICIANUS MARIE ROUWS, CNPAB; JERRI EDSON ZILLI, CNPAB; MARCIA MARIA PARMA LEME, CNPMA; ITAMAR SOARES DE MELO, CNPMA; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Bradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, v. 66, n. 8, p. 3078-3087, 2016. |
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DOI: |
http://dx.doi.org/10.1099/ijsem.0.001148 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense, and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium. Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5 % nucleotide identity with other members of the genus Bradyrhizobium. Average nucleotide identity (ANI) of genome sequences between strain BR 446T and B.huanghuaihaiense was below the threshold for species circumscription (90.7 %). DNA-DNA hybridization resulted in ΔTm values over 6.7 °C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manausense than with B.huanghuaihaiense, indicating a different evolutionary history for nitrogen-fixation genes. Morpho-physiological, genotypic and genomic data supported that these BR strains represent a novel species for which the name Bradyrhizobium stylosanthis sp. nov. is suggested. The type strain is BR 446T (=CNPSo 2823T=HAMBI 3668T=H-8T), isolated from Stylosanthes guianensis. MenosAbstract: The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense, and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium. Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5 % nucleotide identity with other members of the genus Bradyrhizobium. Average nucleotide identity (ANI) of genome sequences between strain BR 446T and B.huanghuaihaiense was below the threshold for species circumscription (90.7 %). DNA-DNA hybridization resulted in ΔTm values over 6.7 °C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manau... Mostrar Tudo |
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Palavras-Chave: |
Bradyrhizobium stylosanthis. |
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Thesagro: |
Fixação simbiótica de nitrogênio; Planta forrageira; Rhizobium. |
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Thesaurus Nal: |
Nitrogen-fixing bacteria; Stylosanthes. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02886naa a2200289 a 4500
001 2062548
005 2020-10-19
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1099/ijsem.0.001148$2DOI
100 1 $aDELAMUTA, J. R. M.
245 $aBradyrhizobium stylosanthis sp. nov., comprising nitrogen-fixing symbionts isolated from nodules of the tropical forage legume Stylosanthes spp.$h[electronic resource]
260 $c2016
520 $aAbstract: The introduction of legumes and nitrogen-fixing bacteria in tropical areas under pasture is a key factor for improvement of soil fertility. However, there are still very few studies concerning the symbionts of tropical forage legumes. We performed a polyphasic study with three strains representing the genus Bradyrhizobium (BR 446T, BR 510 and BR 511) isolated from the tropical perennial forage legume of the genus Stylosanthes. On the basis of 16S rRNA gene sequences, the three strains showed highest similarity with B. huanghuaihaiense, and in the analysis of the intergenic transcribed spacer (ITS) they showed less than 93.4 % similarity to all described species of the genus Bradyrhizobium. Multilocus sequence analysis (MLSA) with three, four or five (dnaK, glnII, gyrB, recA and rpoB) housekeeping genes confirmed that the BR strains belong to a distinct clade, with <96.5 % nucleotide identity with other members of the genus Bradyrhizobium. Average nucleotide identity (ANI) of genome sequences between strain BR 446T and B.huanghuaihaiense was below the threshold for species circumscription (90.7 %). DNA-DNA hybridization resulted in ΔTm values over 6.7 °C with the most closely related species. Similarities among the BR strains and differences from other species were confirmed by rep-PCR analysis. Interestingly, the BR strains were grouped in the analysis of nifH and nodC genes, but showed higher similarity with B. iriomotense and B. manausense than with B.huanghuaihaiense, indicating a different evolutionary history for nitrogen-fixation genes. Morpho-physiological, genotypic and genomic data supported that these BR strains represent a novel species for which the name Bradyrhizobium stylosanthis sp. nov. is suggested. The type strain is BR 446T (=CNPSo 2823T=HAMBI 3668T=H-8T), isolated from Stylosanthes guianensis.
650 $aNitrogen-fixing bacteria
650 $aStylosanthes
650 $aFixação simbiótica de nitrogênio
650 $aPlanta forrageira
650 $aRhizobium
653 $aBradyrhizobium stylosanthis
700 1 $aRIBEIRO, R. A.
700 1 $aARAUJO, J. L. S. de
700 1 $aROUWS, L. F. M.
700 1 $aZILLI, J. E.
700 1 $aPARMA, M. M.
700 1 $aMELO, I. S. de
700 1 $aHUNGRIA, M.
773 $tInternational Journal of Systematic and Evolutionary Microbiology$gv. 66, n. 8, p. 3078-3087, 2016.
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Embrapa Meio Ambiente (CNPMA) |
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| 41. |  | PINTO, C. A. G.; KRZYZANOWSKI, F. C.; FRANÇA-NETO, J. B.; DOURADO-NETO, D.; SILVA, C. B. da; MARCOS-FILHO, J. Relationship between size and physiological potential of soya bean seeds under variations in water availability. Seed Science and Technology, v. 46, n. 3, p. 497-510, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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