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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
30/06/2021 |
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Data da última atualização: |
30/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, C. M. dos; RIBEIRO, N. V. S.; SCHWAB, S.; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
CARLOS M. DOS SANTOS, Bolsista da Embrapa Agrobiologia; NÁTHALIA V. S. RIBEIRO, Bolsista da Embrapa Agrobiologia; STEFAN SCHWAB, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
The effect of inoculation of a diazotrophic bacterial xonsortium on the indigenous bacterial community structure of sugarcane apoplast fluid. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Current Microbiologym, Published online, 25 june 2021. |
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ISSN: |
1432-0991 |
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DOI: |
https://doi.org/10.1007/s00284-021-02571-0 |
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Idioma: |
Inglês |
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Conteúdo: |
The extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. Analysis to evaluate the occurrence of inoculated strains in the recovered data was not conclusive since the ASVs taxonomically close to the inoculated bacteria were observed in low abundance. The present study is the first report to elucidate the bacterial community in sugarcane apoplast fluid using a culture-independent approach. It demonstrated that the diazotrophic bacterial consortium interferes in the natural bacterial community in sugarcane variety RB867515 MenosThe extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. A... Mostrar Tudo |
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Palavras-Chave: |
Endophytic bacteria; Plant growth promotion bacteria. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02707naa a2200217 a 4500
001 2132685
005 2021-06-30
008 2021 bl uuuu u00u1 u #d
022 $a1432-0991
024 7 $ahttps://doi.org/10.1007/s00284-021-02571-0$2DOI
100 1 $aSANTOS, C. M. dos
245 $aThe effect of inoculation of a diazotrophic bacterial xonsortium on the indigenous bacterial community structure of sugarcane apoplast fluid.$h[electronic resource]
260 $c2021
520 $aThe extracellular space in plants, termed the apoplast, has a pH and sugar content that enables bacterial growth and represents a possible niche for the establishment of endophytic bacteria. Previous studies have investigated the effects of diazotrophic bacteria inoculation in sugarcane varieties, but it has not yet been analyzed how the microbial community of apoplast fluid of sugarcane is affected. High-throughput next generation sequencing of the 16S rRNA gene was used throughout this study to determine the effect of inoculation with a diazotrophic bacteria consortium, previously isolated from sugarcane, on the native bacterial population of sugarcane variety RB867515 grown in the field. The analyses were carried out 450 days after inoculation. The results revealed the presence of 22 phyla, with predominance of Proteobacteria phylum. It was observed that the inoculated consortium changed the indigenous bacterial community structure of sugarcane apoplast fluid by decreasing diversity and evenness, interfering in the composition of rare species. Microbial community composition analysis revealed differences between treatments. The differential abundance test showed there were 43 amplicon sequence variants (ASVs) which were relatively more abundant in the inoculated treatment, with predominance of the Sphingomonas genus. The pre-dicted functions of the most abundant ASVs revealed the presence of genera related to plant growth promotion and protection against phytopathogens. Analysis to evaluate the occurrence of inoculated strains in the recovered data was not conclusive since the ASVs taxonomically close to the inoculated bacteria were observed in low abundance. The present study is the first report to elucidate the bacterial community in sugarcane apoplast fluid using a culture-independent approach. It demonstrated that the diazotrophic bacterial consortium interferes in the natural bacterial community in sugarcane variety RB867515
653 $aEndophytic bacteria
653 $aPlant growth promotion bacteria
700 1 $aRIBEIRO, N. V. S.
700 1 $aSCHWAB, S.
700 1 $aBALDANI, J. I.
700 1 $aVIDAL, M. S.
773 $tCurrent Microbiologym, Published online, 25 june 2021.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 102 | |
| 3. |  | DIAS, W. P.; RIBEIRO, N. R.; PIVATO, A.; MOLINA, D. Avaliação da reação de genótipos de soja ao nematóide das lesões radiculares (Pratylenchus brachyurus). In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 30., 2008, Rio Verde. Resumos... Londrina: Embrapa Soja, 2008. p. 137-139. (Embrapa Soja. Documentos, 304). Organizado por Odilon Ferreira Saraiva, César de Castro, Regina Maria Villas Bôas de Campos Leite, Fábio Alvares de Oliveira.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 10. | | FRANCISCO, A.; DIAS, W. P.; RIBEIRO, N. R. Reação de genótipos de milho A Meloidogyne incognita, RAÇA 3 E M. javanica. Nematologia Brasileira, Piracicaba, v. 31, n. 2, p. 111, ago. 2007. Edição dos Resumos apresentado no XXVII Congresso Brasileiro de Nematologia, Goiânia, maio, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Soja. |
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| 13. | | RIBEIRO, N. R.; SILVA, J. F. V.; FRANCISCO, A.; GOMES, J.; MEIRELLES, W. F. Avaliação da resistência de genótipos de milho (Zea mays) à Meloidogyne javanica e M. incognita raça 3. In: CONGRESSO BRASILEIRO DE SOJA, 2.; MERCOSOJA 2002, 2002, Foz do Iguaçu. Perspectivas do agronegócio da soja: resumos. Londrina: Embrapa Soja, 2002. p. 311. (Embrapa Soja. Documentos, 181). Organizado por Odilon Ferreira Saraiva, Clara Beatriz Hoffmann-Campo.| Biblioteca(s): Embrapa Soja. |
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| 14. | | LONDERO, P. M. G.; RIBEIRO, N. D.; POERSCH, N. L.; ANTUNES, I. F.; NÖRENBERG, J. L. Análise de frações de fibra alimentar em cultivares de feijão cultivado em dois ambientes. Ciência Rural, Santa Maria, v. 38, n. 7, p. 2033-2036, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 16. | | RIBEIRO, N. R.; DIAS, W. P.; HOMECHIN, M.; SILVA, J. F. V.; FRANCISCO, A. Avaliação da reação de espécies vegetais ao nematóide das lesões radiculares. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 29., 2007, Campo Grande, MS. Resumos... Londrina: Embrapa Soja, 2007. p. 64-65. (Embrapa Soja. Documentos, 287). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite, Simone Ery Grosskopf.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 17. | | RIBEIRO, N. R.; DIAS, W. P.; HOMECHIN, M.; SILVA, J. F. V.; FRANCISCO, A. Avaliação da reação de genótipos de soja ao nematóide das lesões radiculares In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 29., 2007, Campo Grande, MS. Resumos... Londrina: Embrapa Soja, 2007. p. 62-63. (Embrapa Soja. Documentos, 287). Organizado por Odilon Ferreira Saraiva, Regina Maria Villas Boas de Campos Leite, Simone Ery Grosskopf.| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | RIBEIRO, N. R.; COELHO, FILHO, M. A.; SILVA, T. S. M. da; COELHO, E. F. Comparação entre métodos de estimativa da evapotranspiração de referência em Cruz das Almas, BA, em escala diária e decendial. In: CONGRESSO BRASILEIRO DE ENGENHARIA AGRÍCOLA, 36., 2007, Bonito. Bonito: [s.n.], 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 102 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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