Data/hora: 08/06/2024 01:21:07

Biblioteca(s): | Embrapa Café.
Data corrente: | 28/09/2020
Data da última atualização: | 28/09/2020
Autoria: | BATISTA, D. S.; CASTRO, K. M. de; RIBEIRO, D. M.; CAIXETA, E. T.; SANTOS, M. de O.; VICCINI, L. F.; OTONI, W. C.
Afiliação: | DIEGO SILVA BATISTA, UNIVERSIDADE FEDERAL DE VIÇOSA; KAMILA MOTTA DE CASTRO, UNIVERSIDADE FEDERAL DE VIÇOSA; DIMAS MENDES RIBEIRO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA MOURA, CNPCa; MARCELO DE OLIVEIRA SANTOS, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; LYDERSON FACIO VICCINI, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; WAGNER CAMPOS OTONI, UNIVERSIDADE FEDERAL DE JUIZ DE FORA.
Título: | Ethylene Responses and ACC oxidase Gene Expression in Lippia alba (Verbenaceae) Chemotypes with Varying Ploidy Levels.
Ano de publicação: | 2017
Fonte/Imprenta: | In Vitro Cellular & Developmental Biology - Plant, v. 53, n. 3, p. 278-284, 2017.
DOI: | 10.1007/s11627-017-9827-4
Idioma: | Inglês
Conteúdo: | Lippia alba (Mill.) N. E. Br ex Britton & P. Wilson is a species of great economic importance due to the medicinal activity conferred by its essential oils. Ethylene (ET) is a gaseous hormone that affects several processes in plant growth and development. This hormone is synthesized through a reaction in which 1-aminocyclopropane-1-carboxylic acid (ACC) is converted to ET by the action of the enzyme ACC oxidase (ACO). The ET activity in three L. alba chemotypes (BGEN-01, triploid; BGEN-02, diploid; and BGEN-42, hexaploid) was evaluated by culturing plants in vitro in the presence of the ET precursor (ACC), scavenger mercury perchloride (MP), the inhibitors aminoethoxyvinylglycine (AVG) and silver thiosulfate (STS), and a CO2-enriched atmosphere. Leaf abscission rate, ET accumulation, and ACO gene expression (LaACO) were investigated. Leaf abscission was higher in BGEN-42, regardless of the precursor/inhibitor. ACC had the highest ET accumulation in BGEN-02 and BGEN-01, whereas BGEN-42 presented lower ET accumulation than the control. MP and AVG were effective in preventing ET production when compared to the control. LaACO was upregulated in BGEN-01 and BGEN-42 treated with ACC and in BGEN-01 treated with STS. In the CO2 enrichment experiments, LaACO expression was higher in BGEN-42 in the treatments without forced ventilation. Thus, the expression of this gene in L. alba can be altered by elevating CO2 levels and also by the addition of ACC. This is the first report of ET interactions in L. alba cultured in vitro.
Palavras-Chave: | Forced ventilation.
Thesaurus Nal: | Carbon dioxide; Leaf abscission; Polyploidy.
Categoria do assunto: | --
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (1436-1) - UPC - AP - DD -  -  -  - DIS