Data/hora: 27/05/2024 11:49:40

Biblioteca(s): 	Embrapa Semiárido; Embrapa Uva e Vinho.	
Data corrente: 	28/04/2015	
Data da última atualização: 	03/05/2017	
Autoria: 	PORTO, D. D.; BRUNEAU, M.; PERINI, P.; ANZANELLO, R.; RENOU, J.; SANTOS, H. P. dos; FIALHO, F. B.; REVERS, L. F.	
Afiliação: 	DIOGO DENARDI PORTO, CPATSA; HENRIQUE PESSOA DOS SANTOS, CNPUV; FLAVIO BELLO FIALHO, CNPUV; LUIS FERNANDO REVERS, CNPUV.	
Título: 	Transcription profiling of the chilling requirement for bud break in apples: a putative role for FLC-like genes.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Journal of experimental botany, Oxford, Inglaterra, v. 66, n. 9, p. 2659-2672, May 2015.	
Idioma: 	Português	
Notas: 	doi:10.1093/jxb/erv06	
Conteúdo: 	Apple production depends on the fulfilment of a chilling requirement for bud dormancy release. Insufficient winter chilling results in irregular and suboptimal bud break in the spring, with negative impacts on apple yield. Trees from apple cultivars with contrasting chilling requirements for bud break were used to investigate the expression of the entire set of apple genes in response to chilling accumulation in the field and controlled conditions. Total RNA was analysed on the AryANE v.1.0 oligonucleotide microarray chip representing 57 000 apple genes. The data were tested for functional enrichment, and differential expression was confirmed by real-time PCR. The largest number of dif ferentially expressed genes was found in samples treated with cold temperatures. Cold exposure mostly repressed expression of transcripts related to photosynthesis, and long-term cold exposure repressed flavonoid biosynthesis genes. Among the differentially expressed selected candidates, we identified genes whose annotations were related to the circadian clock, hormonal signalling, regulation of growth, and flower development. Two genes, annotated as FLOWERING LOCUS C-like and MADS AFFECTING FLOWERING , showed strong differential expression in several comparisons. One of these two genes was upregulated in most comparisons involving dormancy release, and this gene?s chromosomal position co-localized with the confidence interval of a major quantitative trait locus for the tim ing of bud break. These results indicate that photosynthesis and auxin transport are major regulatory nodes of apple dormancy and unveil strong candidates for the control of bud dormancy.	
Palavras-Chave: 	Bud dormancy; Castel Gala; FLC-like; Royal Gala.	
Thesagro: 	Maçã.	
Thesaurus Nal: 	Apples; chilling requirement.	
Categoria do assunto: 	A Sistemas de Cultivo	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/123044/1/REVERS-journalExpBotanyAdvAccess-2015.pdf	
Registro original: 	Embrapa Uva e Vinho (CNPUV)	
Exemplares: 	CNPUV - (15833-1) - UMT - AP - DD - 634.11 -  -  - DIS
CPATSA - (55456-1) - UPC - AP - DD -  -  -  - DIS