Data/hora: 29/05/2024 12:59:04

Biblioteca(s): 	Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	17/09/2020	
Data da última atualização: 	16/12/2020	
Autoria: 	NERY, F. M. B.; MELO, F. L.; BOITEUX, L. S.; RIBEIRO, S. da G.; RESENDE, R. O.; ORÍLIO, A. F.; BATISTA, J. G.; LIMA, M. F.; PEREIRA-CARVALHO, R. C.	
Afiliação: 	FLÁVIA M. B. NERY, UNIVERSIDADE DE BRASÍLIA; FERNANDO L. MELO, UNIVERSIDADE DE BRASÍLIA; LEONARDO SILVA BOITEUX, CNPH; SIMONE DA GRACA RIBEIRO, Cenargen; RENATO O. RESENDE, UNIVERSIDADE DE BRASÍLIA; ANELISE F. ORÍLIO, UNIVERSIDADE DE BRASÍLIA; JOSIANE G. BATISTA, UNIVERSIDADE DE BRASÍLIA; MIRTES FREITAS LIMA, CNPH; RITA C. PEREIRA-CARVALHO, UNIVERSIDADE DE BRASÍLIA.	
Título: 	Molecular characterization of Hovenia Dulcis-associated virus 1 (HDaV1) and 2 (HDaV2): new tentative species within the Order Picornavirales.	
Ano de publicação: 	2020	
Fonte/Imprenta: 	Viruses, v. 12, n. 9, 950, 2020	
DOI: 	https://doi.org/10.3390/v12090950	
Idioma: 	Inglês	
Conteúdo: 	In a systematic field survey for plant-infecting viruses, leaf tissues were collected from trees showing virus-like symptoms in Brazil. After viral enrichment, total RNA was extracted and sequenced using the MiSeq platform (Illumina). Two nearly full-length picorna-like genomes of 9534 and 8158 nucleotides were found associated with Hovenia dulcis (Rhamnaceae family). Based upon their genomic information, specific primers were synthetized and used in RT-PCR assays to identify plants hosting the viral sequences. The larger contig was tentatively named as Hovenia dulcis-associated virus 1 (HDaV1), and it exhibited low nucleotide and amino acid identities with Picornavirales species. The smaller contig was related to insect-associated members of the Dicistroviridae family but exhibited a distinct genome organization with three non-overlapping open reading frames (ORFs), and it was tentatively named as Hovenia dulcis-associated virus 2 (HDaV2). Phylogenetic analysis using the amino acid sequence of RNA-dependent RNA polymerase (RdRp) revealed that HDaV1 and HDaV2 clustered in distinct groups, and both viruses were tentatively assigned as new members of the order Picornavirales. HDaV2 was assigned as a novel species in the Dicistroviridae family. The 50 ends of both viruses are incomplete. In addition, a nucleotide composition analysis (NCA) revealed that HDaV1 and HDaV2 have similarities with invertebrate-infecting viruses, suggesting that the primary host(s) of these novel virus species remains to be discovered.	
Palavras-Chave: 	HDaV1; HDaV2; Virome.	
Thesagro: 	Hovenia Dulcis.	
Thesaurus Nal: 	Metagenomics; Picornavirales; Viruses.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/219297/1/ARTIGO-MolecularCharacterizationHoveniaDulcis.pdf	
Registro original: 	Embrapa Hortaliças (CNPH)	
Exemplares: 	CENARGEN - (38268-1) - UPC - AP - DD -  -  -  - DIS
CNPH - (41441-1) - UPC - AP - DD -  -  -  - DIS