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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2000 |
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Data da última atualização: |
27/12/2000 |
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Autoria: |
REIS, V. M.; REIS JÚNIOR, F. B. dos; SALLES, J. F.; SCHLOTER, M. |
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Título: |
Characterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.). |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Symbiosis, Rehovot, v. 29, p. 139-150, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Immunological techniques can be applied to detect micro-organisms in different ecosystems. To follow the fate of Herbaspirillum seropedicae strain Z67 and H . rubrisubalbicans strain M4 in the rhizosphere of elephant grass, Pennisetum purpureum Schun. var. Capim Cana D'Africa, polyclonal antisera were raised against both strains. Both antisera were purified with protein-A, followed by a primary characterization for cross-reactivity. To reduce cross-reactivity for both sera, affinity purification was used for improvement. After that, both sera could be considered as species specific. The detection limit was 105 cells ml-l for the anti H. rubrisubalbicans serum and 1Q6 cells ml-l for the anti H. seropedicae serum. It was shown that two months after inoculation, both strains could be detected in the rhizoplane of elephant grass in high densities. The estimated numbers by ELISA were 107 cells g-1 fresh weight. |
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Palavras-Chave: |
Bactéira diazotrófica; Diazotrophic bacteria; Elephant grass. |
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Thesagro: |
Capim Elefante; Gramínea Forrageira; Pennisetum Purpureum. |
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Thesaurus Nal: |
forage grasses; Herbaspirillum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01690naa a2200253 a 4500
001 1597264
005 2000-12-27
008 2000 bl --- 0-- u #d
100 1 $aREIS, V. M.
245 $aCharacterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.).
260 $c2000
520 $aImmunological techniques can be applied to detect micro-organisms in different ecosystems. To follow the fate of Herbaspirillum seropedicae strain Z67 and H . rubrisubalbicans strain M4 in the rhizosphere of elephant grass, Pennisetum purpureum Schun. var. Capim Cana D'Africa, polyclonal antisera were raised against both strains. Both antisera were purified with protein-A, followed by a primary characterization for cross-reactivity. To reduce cross-reactivity for both sera, affinity purification was used for improvement. After that, both sera could be considered as species specific. The detection limit was 105 cells ml-l for the anti H. rubrisubalbicans serum and 1Q6 cells ml-l for the anti H. seropedicae serum. It was shown that two months after inoculation, both strains could be detected in the rhizoplane of elephant grass in high densities. The estimated numbers by ELISA were 107 cells g-1 fresh weight.
650 $aforage grasses
650 $aHerbaspirillum
650 $aCapim Elefante
650 $aGramínea Forrageira
650 $aPennisetum Purpureum
653 $aBactéira diazotrófica
653 $aDiazotrophic bacteria
653 $aElephant grass
700 1 $aREIS JÚNIOR, F. B. dos
700 1 $aSALLES, J. F.
700 1 $aSCHLOTER, M.
773 $tSymbiosis, Rehovot$gv. 29, p. 139-150, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
07/03/2017 |
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Data da última atualização: |
09/04/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LIMA, A. O. D.; OLIVEIRA, P. S. N.; TIZIOTO, P. C.; SOMAVILLA, A. L.; DINIZ, W. J. S.; SILVA, J. V. D.; ANDRADE, S. C. S.; BOSCHIERO, C.; CESAR, A. S. M.; SOUZA, M. M.; ROCHA, M. I. P.; AFONSO, J.; BUSS, C. E.; MUDADU, M. de A.; MOURAO, G. B.; COUTINHO, L. L.; REGITANO, L. C. de A. |
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Afiliação: |
A. O. D. LIMA, UFSCar; P. S. N. OLIVEIRA, CPPSE; P. C. TIZIOTO, CPPSE; A. L. SOMAVILLA, Unesp; W. J. S. DINIZ, UFSCar; J. V. D. SILVA, UFSCar; S. C. S. ANDRADE, USP; C. BOSCHIERO, Esalq/USP; A. S. M. CESAR, Esalq/USP; M. M. SOUZA, UFSCar; M. I. P. ROCHA, UFSCar; J. AFONSO, UFSCar; C. E. BUSS, UFSCar; MAURICIO DE ALVARENGA MUDADU, CNPTIA; G. B. MOURAO, Esalq/USP; L. L. COUTINHO, Esalq/USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
PRUNE2 gene has a potential effect on residual feed intake in Nellore cattle. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Journal of Animal Science, v. 94, p. 152-153, 2016. |
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Páginas: |
p. 148-149. |
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DOI: |
10.2527/jam2016-0318 |
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Idioma: |
Inglês |
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Notas: |
Supplement 5. |
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Conteúdo: |
Residual feed intake (RFI) can increase the profitability of producers, reduce methane emission and land allocation to livestock production. However, this trait has late and costly measurements. Identifying gene expression changes combined with polymorphisms that affect residual feed intake variation is important for identify target regulatory polymorphisms that can be used in animal breeding programs. Diverse studies performed by our research group in a Nellore population, such as genome-wide association (GWA), association weight matrix (AWM) and RNA-seq analysis of liver tissue have been pointed Prune homolog 2 (Drosophila) (PRUNE2) as a potential candidate gene influencing feed efficiency. For this reason, we select this gene for a more detailed analysis considering haplotypes consisting of SNPs presented in the Illumina Bovine HD Bead Chip. |
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Palavras-Chave: |
Functional gene enrichment. |
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Thesagro: |
Gado de corte; Gado Nelore; Genética. |
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Thesaurus NAL: |
Beef cattle; Feed conversion; Genetics; Haplotypes. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02057nam a2200433 a 4500
001 2066412
005 2019-04-09
008 2016 bl uuuu u00u1 u #d
024 7 $a10.2527/jam2016-0318$2DOI
100 1 $aLIMA, A. O. D.
245 $aPRUNE2 gene has a potential effect on residual feed intake in Nellore cattle.$h[electronic resource]
260 $aJournal of Animal Science, v. 94, p. 152-153$c2016
300 $ap. 148-149.
500 $aSupplement 5.
520 $aResidual feed intake (RFI) can increase the profitability of producers, reduce methane emission and land allocation to livestock production. However, this trait has late and costly measurements. Identifying gene expression changes combined with polymorphisms that affect residual feed intake variation is important for identify target regulatory polymorphisms that can be used in animal breeding programs. Diverse studies performed by our research group in a Nellore population, such as genome-wide association (GWA), association weight matrix (AWM) and RNA-seq analysis of liver tissue have been pointed Prune homolog 2 (Drosophila) (PRUNE2) as a potential candidate gene influencing feed efficiency. For this reason, we select this gene for a more detailed analysis considering haplotypes consisting of SNPs presented in the Illumina Bovine HD Bead Chip.
650 $aBeef cattle
650 $aFeed conversion
650 $aGenetics
650 $aHaplotypes
650 $aGado de corte
650 $aGado Nelore
650 $aGenética
653 $aFunctional gene enrichment
700 1 $aOLIVEIRA, P. S. N.
700 1 $aTIZIOTO, P. C.
700 1 $aSOMAVILLA, A. L.
700 1 $aDINIZ, W. J. S.
700 1 $aSILVA, J. V. D.
700 1 $aANDRADE, S. C. S.
700 1 $aBOSCHIERO, C.
700 1 $aCESAR, A. S. M.
700 1 $aSOUZA, M. M.
700 1 $aROCHA, M. I. P.
700 1 $aAFONSO, J.
700 1 $aBUSS, C. E.
700 1 $aMUDADU, M. de A.
700 1 $aMOURAO, G. B.
700 1 $aCOUTINHO, L. L.
700 1 $aREGITANO, L. C. de A.
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Embrapa Pecuária Sudeste (CPPSE) |
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