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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2000 |
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Data da última atualização: |
27/12/2000 |
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Autoria: |
REIS, V. M.; REIS JÚNIOR, F. B. dos; SALLES, J. F.; SCHLOTER, M. |
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Título: |
Characterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.). |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Symbiosis, Rehovot, v. 29, p. 139-150, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Immunological techniques can be applied to detect micro-organisms in different ecosystems. To follow the fate of Herbaspirillum seropedicae strain Z67 and H . rubrisubalbicans strain M4 in the rhizosphere of elephant grass, Pennisetum purpureum Schun. var. Capim Cana D'Africa, polyclonal antisera were raised against both strains. Both antisera were purified with protein-A, followed by a primary characterization for cross-reactivity. To reduce cross-reactivity for both sera, affinity purification was used for improvement. After that, both sera could be considered as species specific. The detection limit was 105 cells ml-l for the anti H. rubrisubalbicans serum and 1Q6 cells ml-l for the anti H. seropedicae serum. It was shown that two months after inoculation, both strains could be detected in the rhizoplane of elephant grass in high densities. The estimated numbers by ELISA were 107 cells g-1 fresh weight. |
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Palavras-Chave: |
Bactéira diazotrófica; Diazotrophic bacteria; Elephant grass. |
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Thesagro: |
Capim Elefante; Gramínea Forrageira; Pennisetum Purpureum. |
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Thesaurus Nal: |
forage grasses; Herbaspirillum. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01690naa a2200253 a 4500
001 1597264
005 2000-12-27
008 2000 bl --- 0-- u #d
100 1 $aREIS, V. M.
245 $aCharacterisation of different polyclonal antisera to quantify Herbaspirillum spp. in elephant grass (Pennisetum purpureum Schun.).
260 $c2000
520 $aImmunological techniques can be applied to detect micro-organisms in different ecosystems. To follow the fate of Herbaspirillum seropedicae strain Z67 and H . rubrisubalbicans strain M4 in the rhizosphere of elephant grass, Pennisetum purpureum Schun. var. Capim Cana D'Africa, polyclonal antisera were raised against both strains. Both antisera were purified with protein-A, followed by a primary characterization for cross-reactivity. To reduce cross-reactivity for both sera, affinity purification was used for improvement. After that, both sera could be considered as species specific. The detection limit was 105 cells ml-l for the anti H. rubrisubalbicans serum and 1Q6 cells ml-l for the anti H. seropedicae serum. It was shown that two months after inoculation, both strains could be detected in the rhizoplane of elephant grass in high densities. The estimated numbers by ELISA were 107 cells g-1 fresh weight.
650 $aforage grasses
650 $aHerbaspirillum
650 $aCapim Elefante
650 $aGramínea Forrageira
650 $aPennisetum Purpureum
653 $aBactéira diazotrófica
653 $aDiazotrophic bacteria
653 $aElephant grass
700 1 $aREIS JÚNIOR, F. B. dos
700 1 $aSALLES, J. F.
700 1 $aSCHLOTER, M.
773 $tSymbiosis, Rehovot$gv. 29, p. 139-150, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 561 | |
| 125. |  | SILVESTRE, D. A.; XAVIER, J.; SANTOS, L. A.; REIS, V. M. Análise biométrica da cana-de-açúcar cultivada em diferentes proporções de NO3- / NH4 In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., FERTBIO 2016. Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 139. | | OLIVEIRA, S. M.; PITARD, R. M.; REIS, V. M.; FARIA, S. M. de; STRALIOTTO, R. Capacidade de nodulação e promoção de crescimento por isolados de Burkholderia spp. em associação com Mimosa pigra e Mimosa pudica. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24.; SIMPÓSIO BRASILEIRO DE MICROBACTÉRIAS, 12.; SIMPÓSIO DE COLEÇÕES DE CULTURA, 2.; ENCONTRO DE ENSINO EM MICROBIOLOGIA, 4., 2007, Brasília, DF. Anais. {São Paulo}: SBM, 2007. 1 p. 1 CD-ROM. Parceria: UFLA| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 561 | |
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