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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
18/02/2021 |
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Data da última atualização: |
18/02/2021 |
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Tipo da produção científica: |
Orientação de Tese de Pós-Graduação |
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Autoria: |
REIS, L. de N. A. dos. |
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Afiliação: |
LUCIANE DE NAZARÉ ALMEIDA DOS REIS, UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Metagenomic analysis of the begomovirus diversity in tomatoes in Central Brazil and impact of the Ty-1 tolerance gene on viral evolutionary dynamics. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
2020. |
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Páginas: |
205 f. |
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Idioma: |
Inglês |
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Notas: |
Thesis (Doutor in Phytopathology). Universidade de Brasília, Brasília, DF. Orientadora: Rita de Cássia Pereira Carvalho. Co-orientador: Leonardo Silva Boiteux, Embrapa Hortaliças. |
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Conteúdo: |
The general objective of the present work was to conduct metagenomics studies on the diversity of begomoviruses in tomatoes in Brazil. The specific objectives were: (a) to carry out comparative studies of the begomovirus diversity infecting tomato plants with and without the Ty?1 gene and (b) to catalog the predominant viral species and/or new begomoviruses occurring in tomato plants with and without the Ty?1 gene. For this, 107 samples were collected in production fields in Goiás (n = 56), Distrito Federal (n = 27) and Minas Gerais (n = 24) between the years 2002 and 2016. The total DNA of the samples was extracted and submitted to PCR using primers to detect begomovirus and also with primers for the genomic region linked to the Ty?1 gene. Subsequently, the samples were subjected to enrichment via RCA (Rolling Circle Amplification) and divided into two pools: tomatoes without the Ty?1 gene (n = 64) and with the Ty?1 gene (n = 43). The two pools were sequenced on an Illumina HiSeq 2500 platform. The obtained sequences were assembled using the CLC Genomics Workbench 11.0 program and analyzed in Geneious 10.1. The sequences were then compared to viral sequences present on GenBank using the BLASTn algorithm. |
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Thesagro: |
Resistência Genética; Tomate. |
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Thesaurus Nal: |
Begomovirus; Solanum lycopersicum var. lycopersicum. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/221266/1/2020-LucianedeNazareAlmeidadosReis.pdf
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Marc: |
LEADER 01987nam a2200181 a 4500
001 2130091
005 2021-02-18
008 2020 bl uuuu m 00u1 u #d
100 1 $aREIS, L. de N. A. dos
245 $aMetagenomic analysis of the begomovirus diversity in tomatoes in Central Brazil and impact of the Ty-1 tolerance gene on viral evolutionary dynamics.$h[electronic resource]
260 $a2020.$c2020
300 $a205 f.
500 $aThesis (Doutor in Phytopathology). Universidade de Brasília, Brasília, DF. Orientadora: Rita de Cássia Pereira Carvalho. Co-orientador: Leonardo Silva Boiteux, Embrapa Hortaliças.
520 $aThe general objective of the present work was to conduct metagenomics studies on the diversity of begomoviruses in tomatoes in Brazil. The specific objectives were: (a) to carry out comparative studies of the begomovirus diversity infecting tomato plants with and without the Ty?1 gene and (b) to catalog the predominant viral species and/or new begomoviruses occurring in tomato plants with and without the Ty?1 gene. For this, 107 samples were collected in production fields in Goiás (n = 56), Distrito Federal (n = 27) and Minas Gerais (n = 24) between the years 2002 and 2016. The total DNA of the samples was extracted and submitted to PCR using primers to detect begomovirus and also with primers for the genomic region linked to the Ty?1 gene. Subsequently, the samples were subjected to enrichment via RCA (Rolling Circle Amplification) and divided into two pools: tomatoes without the Ty?1 gene (n = 64) and with the Ty?1 gene (n = 43). The two pools were sequenced on an Illumina HiSeq 2500 platform. The obtained sequences were assembled using the CLC Genomics Workbench 11.0 program and analyzed in Geneious 10.1. The sequences were then compared to viral sequences present on GenBank using the BLASTn algorithm.
650 $aBegomovirus
650 $aSolanum lycopersicum var. lycopersicum
650 $aResistência Genética
650 $aTomate
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Registro original: |
Embrapa Hortaliças (CNPH) |
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| Registros recuperados : 8 | |
| 4. |  | MICHELOTTO, M. D.; PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; BOITEUX, L. S.; CARREGA, W. C.; LAMANA, L. E. P.; GODOY, I. J. de. Evaluation of pyraclostrobin as a management tool of Groundnut ringspot virus in peanut crop. Phytoparasitica, v. 48, p. 719-726, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 5. | | PEREIRA-CARVALHO, R. de C.; REIS, L. de N. A. dos; NERY, F. M. B.; CARES, J. E.; MICHELOTTO, M. D.; BOITEUX, L. S. Doenças do amendoim (Arachis Hypogoea L.). In: CARREGA, W. C.; ALVES, P. L. da C. A. A cultura do amendoim e seus reflexos econômicos, sociais e técnicos. Jaboticabal: Funep, 2022. p. 145-195.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Hortaliças. |
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| 6. | | REIS, L. de N. A. dos; FONSECA, M. E. de N.; RIBEIRO, S. G.; NAITO, F. Y. B.; BOITEUX, L. S.; PEREIRA-CARVALHO, R. de C. Metagenomics of neotropical single-stranded DNA viruses in tomato cultivars with and without the Ty-1 gene. Viruses, v. 12, n. 8, 819, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | REIS, L. de N. A. dos; BOITEUX, L. S.; FONSECA, M. E. N.; ROJAS, M. R.; GILBERTSON, R. L.; CARVALHO, R. de C. P. Tomato golden net virus and tomato yellow net virus: two novel New World begomoviruses with monopartite genomes. Archives of Virology, v. 168, n. 2, article number 235, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 8. | | OLIVEIRA, I. A. de; REIS, L. de N. A. dos; NERY, F. M. B.; FERNANDES, N. A. N.; GONÇALVES, V. P. M. R.; ROJAS, M. R.; GILBERTSON, R. L.; REIS, A.; BOITEUX, L. S.; FONSECA, M. E. N.; PEREIRA-CARVALHO, R. de C. Genomic characterization of a highly divergent tomato chlorotic mottle Guyane virus strain in the Brazilian Amazon River Basin Phytoparasitica v. 52, n. 13, 2024. 5 p. Na publicação: Maria Esther de Noronha Fonseca‐Boiteux.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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