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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
27/04/2022 |
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Data da última atualização: |
23/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SALVADOR, M. V.; KOCK, F. V. C.; SANTOS, I. L.; PAULINO, J. F. C.; ALMEIDA, C. P. de; GARCIA, R. H. S.; BENCHIMOL-REIS, L. L.; COLNAGO, L. A.; FERREIRA, A. G. |
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Afiliação: |
LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
Monitoring stimulated darkening from UV-C light on different bean genotypes by NMR spectroscopy. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Molecules, v. 27, 2060, 2022. |
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Páginas: |
10 p. |
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DOI: |
https://doi.org/10.3390/molecules27072060 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of UV-C cool white light on bean (Phaseolus vulgaris L.) seeds significantly increases the biochemical seed coat post-harvest darkening process, whilst preserving seed germination. The aim of this work consists in monitoring the effect caused by the incidence of UV-C light on different bean genotypes using NMR spectroscopy. The genotype samples named IAC Alvorada; TAA Dama;BRS Estilo and BRS Pérola from the Agronomic Institute (IAC; Campinas; SP; Brazil) were evaluated.The following two methodologies were used: a prolonged darkening, in which the grain is placed in a room at a controlled temperature (298 K) and humidity for 90 days, simulating the supermarket shelf; an accelerated darkening, where the grains are exposed to UV-C light (254 nm) for 96 h. The experiments were performed using the following innovative time-domain (TD) NMR approaches:the RK-ROSE pulse sequence; one- and two-dimensional high resolution (HR) NMR experiments(1H; 1H-1H COSY and 1H-13C HSQC); chemometrics tools, such as PLS-DA and heat plots. The results suggest that the observed darkening occurs on the tegument after prolonged (90 days) and accelerated (96 h) conditions. In addition, the results indicate that phenylalanine is the relevant metabolite within this context, being able to participate in the chemical reactions accounted for by the darkening processes. Additionally, it is possible to confirm that a UV-C lamp accelerates oxidative enzymatic reactions and that the NMR methods used were a trustworthy approach to monitor and understand the darkening in bean seeds at metabolite level. MenosThe use of UV-C cool white light on bean (Phaseolus vulgaris L.) seeds significantly increases the biochemical seed coat post-harvest darkening process, whilst preserving seed germination. The aim of this work consists in monitoring the effect caused by the incidence of UV-C light on different bean genotypes using NMR spectroscopy. The genotype samples named IAC Alvorada; TAA Dama;BRS Estilo and BRS Pérola from the Agronomic Institute (IAC; Campinas; SP; Brazil) were evaluated.The following two methodologies were used: a prolonged darkening, in which the grain is placed in a room at a controlled temperature (298 K) and humidity for 90 days, simulating the supermarket shelf; an accelerated darkening, where the grains are exposed to UV-C light (254 nm) for 96 h. The experiments were performed using the following innovative time-domain (TD) NMR approaches:the RK-ROSE pulse sequence; one- and two-dimensional high resolution (HR) NMR experiments(1H; 1H-1H COSY and 1H-13C HSQC); chemometrics tools, such as PLS-DA and heat plots. The results suggest that the observed darkening occurs on the tegument after prolonged (90 days) and accelerated (96 h) conditions. In addition, the results indicate that phenylalanine is the relevant metabolite within this context, being able to participate in the chemical reactions accounted for by the darkening processes. Additionally, it is possible to confirm that a UV-C lamp accelerates oxidative enzymatic reactions and that the NMR methods used were... Mostrar Tudo |
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Palavras-Chave: |
Bean darkening; NMR spectroscopy and chemometrics; UV-C irradiation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1142447/1/P-Monitoring-Stimulated-Darkening-from-UV-C-Light-on.pdf
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Marc: |
LEADER 02434naa a2200277 a 4500
001 2142447
005 2024-01-23
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/molecules27072060$2DOI
100 1 $aSALVADOR, M. V.
245 $aMonitoring stimulated darkening from UV-C light on different bean genotypes by NMR spectroscopy.$h[electronic resource]
260 $c2022
300 $a10 p.
520 $aThe use of UV-C cool white light on bean (Phaseolus vulgaris L.) seeds significantly increases the biochemical seed coat post-harvest darkening process, whilst preserving seed germination. The aim of this work consists in monitoring the effect caused by the incidence of UV-C light on different bean genotypes using NMR spectroscopy. The genotype samples named IAC Alvorada; TAA Dama;BRS Estilo and BRS Pérola from the Agronomic Institute (IAC; Campinas; SP; Brazil) were evaluated.The following two methodologies were used: a prolonged darkening, in which the grain is placed in a room at a controlled temperature (298 K) and humidity for 90 days, simulating the supermarket shelf; an accelerated darkening, where the grains are exposed to UV-C light (254 nm) for 96 h. The experiments were performed using the following innovative time-domain (TD) NMR approaches:the RK-ROSE pulse sequence; one- and two-dimensional high resolution (HR) NMR experiments(1H; 1H-1H COSY and 1H-13C HSQC); chemometrics tools, such as PLS-DA and heat plots. The results suggest that the observed darkening occurs on the tegument after prolonged (90 days) and accelerated (96 h) conditions. In addition, the results indicate that phenylalanine is the relevant metabolite within this context, being able to participate in the chemical reactions accounted for by the darkening processes. Additionally, it is possible to confirm that a UV-C lamp accelerates oxidative enzymatic reactions and that the NMR methods used were a trustworthy approach to monitor and understand the darkening in bean seeds at metabolite level.
653 $aBean darkening
653 $aNMR spectroscopy and chemometrics
653 $aUV-C irradiation
700 1 $aKOCK, F. V. C.
700 1 $aSANTOS, I. L.
700 1 $aPAULINO, J. F. C.
700 1 $aALMEIDA, C. P. de
700 1 $aGARCIA, R. H. S.
700 1 $aBENCHIMOL-REIS, L. L.
700 1 $aCOLNAGO, L. A.
700 1 $aFERREIRA, A. G.
773 $tMolecules$gv. 27, 2060, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 207 | |
| 201. |  | CUNHA, A. F.; ANDRADE, H. M.; SOUZA, F. N.; FIALHO JUNIOR, L. C.; ROSA, D. L. S. O.; SANCHEZ, E. M. R.; GIDLUND, M.; GOTO, H.; BRITO, M. A. V. P.; GUIMARÃES, A. S.; LAGE, A. P.; REIS, L. C.; LIBERA, A. M. M. P. D.; HEINEMANN, M. B.; CERQUEIRA, M. M. O. P. Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. Journal of Dairy Science, v. 103, n. 5, p. 4588-4605, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 202. | | REIS, L. O.; GOIS, G. C.; FÉLIX, W. P.; ARAÚJO, C. F.; NUNES, T. S. dos S.; BEZERRA, H. F. C.; RAMOS, E. J. N.; RODRIGUES, A. C.; PENHA, L. G. da; ALMEIDA, B. A. de S.; FERREIRA FILHO, P. A.; ARAUJO, G. G. L. de; MENEZES, D. R.; QUEIROZ, M. A. A. Fermentative profile and nutritional composition of sugarcane silages associated with different levels of moringa hay. Agronomy Journal, v. 114, n. 5, p. 2787-2795, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Semiárido. |
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| 203. | | SILVA, T. F. da; QUADROS, A. P. N. de; RÊGO, G. M. S. do; OLIVEIRA, J. de; MEDEIROS, J. T. de; REIS, L. F. M. dos; RIBEIRO, T. M. P.; CARVALHO, M. V.; MATTOS, P. S. R. de; MATHIAS, L. A.; PALUDO, G. R. Leptospira spp. in free-ranging Capybaras ( Hydrochoerus hydrochaeris) from Midwestern Brazil. Vector-Borne and Zoonotic Diseases, v. 23, n. 3, p. 106-112, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 204. | | OLIVEIRA, I. A. de; REIS, L. de N. A. dos; NERY, F. M. B.; FERNANDES, N. A. N.; GONÇALVES, V. P. M. R.; ROJAS, M. R.; GILBERTSON, R. L.; REIS, A.; BOITEUX, L. S.; FONSECA, M. E. N.; PEREIRA-CARVALHO, R. de C. Genomic characterization of a highly divergent tomato chlorotic mottle Guyane virus strain in the Brazilian Amazon River Basin Phytoparasitica v. 52, n. 13, 2024. 5 p. Na publicação: Maria Esther de Noronha Fonseca‐Boiteux.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Hortaliças. |
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| 205. | | FIGUEIREDO, R. de O.; PEREIRA, A. S.; COSTA, C. F. G.; TORRESAN, F. E.; LEIVAS, J. F.; FILIZOLA, H. F.; SONODA, K. C.; REIS, L. C.; SOUZA, M. D. de; GOMES, M. A. F.; ZUCCARI, M. L.; SILVA, M. S. G. M. e; PICCOLO, M. C.; TANK, M.; CRUZ, P. P. N.; PAMPLIN, P. A. Z.; CAMARGO, P. B. de; BOEIRA, R. C.; BARIZON, R. R. M.; PEREIRA, S. E. M.; PAZIANOTTO, R. A. A.; GREEN, T. R.; FERRACINI, V. L. Land use effects on the ecohydrology in headwater areas of the Jaguari river basin. In: INTERNACIONAL CONFERENCE ON ECOHYDROLOGY FOR SECURITY OF AQUATIC ECOSYSTEMS AND SOCIETIES, 2017, Campos dos Goytacazes. Book of abstracts... Campos dos Goytacazes: UENF, 2017.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 206. | | DAMASCENO JUNIOR, G. A.; ROQUE, F. de O.; GARCIA, L. C.; RIBEIRO, D. B.; TOMAS, W. M.; DIAS, E. S.; DIAS, F. A.; LIBONATI, R.; RODRIGUES, J. A.; SANTOS, F. L. M.; PEREIRA, A. de M. M.; SOUZA, E. B. de; REIS, L. K.; OLIVEIRA, M. da R.; SOUZA, A. H. de A.; PINEDA, D. A. M.; FERREIRA, B. H. dos S.; BORTOLOTTO, I. M.; POTT, A. Lessons to be learned from the wildfire catastrophe of 2020 in the Pantanal wetland. Wetland Science & Practice, v. 38, n. 2, p. 107-115, april 2021.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Pantanal. |
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| 207. | | TOMAS, W. M.; ROQUE, F. de O.; MORATO, R. G.; MÉDICI, P. E.; CHIARAVALLOTI, R. M.; TORTATO, F. R.; PENHA, J. M. F.; IZZO, T. J.; GARCIA, L. C.; LOURIVAL, R. F. F.; GIRARD, P.; ALBUQUERQUE, N. R.; ALMEIDA-GOMES, M.; ANDRADE, M. H. DA S.; ARAÚJO, F. A. S.; ARAÚJO, A. C.; ARRUDA, E. C. DE.; ASSUNÇÃO, V. A.; BATTIROLA, L. D.; BENITES, M.; BOLZAN, F. P.; BOOCK, J. C.; BORTOLOTTO, I. M.; BRASIL, M. DA S.; CAMILO, A. R.; CAMPOS, Z.; CARNIELLO, M. A.; CATELLA, A. C.; CHEIDA, C. C.; CRAWSHAW JR. P. G.; CRISPIM, S. M. A.; DAMASCENO JUNIOR, G. A.; DESBIEZ, A. L. J.; DIAS, F. A.; EATON, D. P.; FAGGIONI, G. P.; FARINACCIO, M. A.; FERNANDES, J. F. A.; FERREIRA, V. L.; FISCHER, E. A.; FRAGOSO, C. E.; FREITAS, G. O.; GALVANI, F.; GARCIA, A. S.; GARCIA, C. M.; GRACIOLLI, G.; GUARIENTO, R. D.; GUEDES, N. M. R.; GUERRA, A.; HERRERA, H. M.; HOOGESTEIJN, R.; IKEDA, S. C.; JULIANO, R. S.; KANTEK, D. L. Z. K.; KEUROGHLIAN, A.; LACERDA, A. C. R.; LACERDA, A. L. R.; LANDEIRO, V. L.; LAPS, R. R.; LAYME, V.; LEIMGRUBER, P.; ROCHA, F. L.; MAMEDE, S.; MARQUES, D. K. S.; MARQUES, M. I.; MATEUS, L. A. F.; MORAES R. N.; MOREIRA, T. A.; MOURAO, G.; NICOLA, R. D.; NOGUEIRA, D. G.; NUNES, A. P.; CUNHA, C. N. DA.; OLIVEIRA, M. D. de; OLIVEIRA, M. R.; PAGGI, G. M.; PELLEGRIN, A. O.; PEREIRA, G. M. F.; PERES, I. A. H. F. S.; PINHO, J. B.; POTT, A.; PROVETE, D. B.; REIS, V. D. A. dos; REIS, L. K. DOS; RENAUD, P. C.; RIBEIRO, D. B.; ROSSETTO, O. C.; SABINO, J.; RUMIZ, D.; SALIS, S. M.; SANTANA, D. J.; SANTOS, S. A.; SARTORI, Â. L.; SATO, M.; SCHUCHMANN, K-L.; SCREMIN-DIAS, E.; SEIXAS, G. H. F.; SEVERO-NETO, F.; SIGRIST, M. R.; SILVA, A.; SILVA, C. J.; SIQUEIRA, A. L.; SORIANO, B. M. A.; SOUSA, L. M.; SOUZA, F. L.; STRUSSMANN, C.; SUGAI, L. S. M.; TOCANTINS, N.; URBANETZ, C.; VALENTE-NETO, F.; VIANA, D. P.; YANOSKY, A.; JUNK, W. J. Sustainability Agenda for the Pantanal Wetland: Perspectives on a Collaborative Interface for Science, Policy, and Decision-Making. Tropical Conservation Science, v. 12, p. 1-30, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 207 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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