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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
20/04/2010 |
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Data da última atualização: |
13/08/2019 |
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Tipo da produção científica: |
Boletim de Pesquisa e Desenvolvimento |
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Autoria: |
NOGUEIRA, M. F.; NETO, A. A. da C.; JULIANO, R. S.; SANTOS, C. J. S.; MONTEZUMA, E. de S.; REIS, J. K. P. dos. |
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Afiliação: |
MARCIA FURLAN NOGUEIRA TAVARES DE LIMA, CPAP; Aparecida Amorim da Costa Neto, IAGRO; RAQUEL SOARES JULIANO, CPAP; CARLOS JOSE SOUSA SANTOS, CPAP; Elisa de Souza Montezuma, UFMS; Jenner Karlisson Pimenta dos Reis, UFMG. |
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Título: |
ELISA rgp90 - metodologia alternativa para o diagnóstico da anemia infecciosa equina no Pantanal. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Corumba: Embrapa Pantanal, 2009. |
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Páginas: |
18 p. |
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Série: |
(Embrapa Pantanal.Boletim de Pesquisa e Desenvolvimento, 93). |
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Idioma: |
Português |
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Conteúdo: |
Como teste oficial para diagnóstico da anemia infecciosa equina (AIE), a política governamental brasileira para prevenção e controle da AIE determina que seja usado o teste de imunodifusão em gel de ágar (IDGA) com a proteína p26 do capsídeo viral como antígeno. A Organização Mundial para a Saúde Animal (OIE) afirma que a IDGA e os testes de imunoadsorção enzimática (ELISAs) são ambos testes acurados e confiáveis, classificando os últimos como testes alternativos. Internacionalmente, há quatro ELISAs disponíveis, três usando a p26, e um a p26 e a gp45, um antígeno da transmembrana viral. Pesquisadores brasileiros desenvolveram um ELISA indireto com uma glicoproteína recombinante da superfície viral, a rgp90. Anticorpos contra a gp90 são usualmente detectados antes dos anticorpos contra a p26, e quando os níveis de replicação viral são extremamente baixos. O objetivo deste estudo preliminar foi comparar o desempenho do ELISA rgp90 com a oficialmente aceita IDGA utilizando a p26, em amostras de equídeos do Pantanal. |
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Palavras-Chave: |
AIE; equídeo; IDGA; muar; retrovírus; sorologia. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/37287/1/BP93.pdf
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Marc: |
LEADER 01803nam a2200265 a 4500
001 1812820
005 2019-08-13
008 2009 bl uuuu u0uu1 u #d
100 1 $aNOGUEIRA, M. F.
245 $aELISA rgp90 - metodologia alternativa para o diagnóstico da anemia infecciosa equina no Pantanal.
260 $aCorumba: Embrapa Pantanal$c2009
300 $a18 p.
490 $a(Embrapa Pantanal.Boletim de Pesquisa e Desenvolvimento, 93).
520 $aComo teste oficial para diagnóstico da anemia infecciosa equina (AIE), a política governamental brasileira para prevenção e controle da AIE determina que seja usado o teste de imunodifusão em gel de ágar (IDGA) com a proteína p26 do capsídeo viral como antígeno. A Organização Mundial para a Saúde Animal (OIE) afirma que a IDGA e os testes de imunoadsorção enzimática (ELISAs) são ambos testes acurados e confiáveis, classificando os últimos como testes alternativos. Internacionalmente, há quatro ELISAs disponíveis, três usando a p26, e um a p26 e a gp45, um antígeno da transmembrana viral. Pesquisadores brasileiros desenvolveram um ELISA indireto com uma glicoproteína recombinante da superfície viral, a rgp90. Anticorpos contra a gp90 são usualmente detectados antes dos anticorpos contra a p26, e quando os níveis de replicação viral são extremamente baixos. O objetivo deste estudo preliminar foi comparar o desempenho do ELISA rgp90 com a oficialmente aceita IDGA utilizando a p26, em amostras de equídeos do Pantanal.
653 $aAIE
653 $aequídeo
653 $aIDGA
653 $amuar
653 $aretrovírus
653 $asorologia
700 1 $aNETO, A. A. da C.
700 1 $aJULIANO, R. S.
700 1 $aSANTOS, C. J. S.
700 1 $aMONTEZUMA, E. de S.
700 1 $aREIS, J. K. P. dos
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Registro original: |
Embrapa Pantanal (CPAP) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
21/08/2008 |
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Data da última atualização: |
22/08/2008 |
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Autoria: |
BROWN, G. G.; MARTINS, P. T.; MATSUMURA, C.; SILVA, S. H. da; FERRARI, E.; KORASAKI, V.; PASINI, A. |
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Título: |
Growth of Pontoscolex corethrurus in an oxisol mixed or not with different amounts of sand and/or organic matter. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: International Symposium on Earthworm Ecology, 8., 2006, Kraków. Abstracts... Kraków: Jagiellonian University, 2006. p. 61. |
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Idioma: |
Inglês |
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Conteúdo: |
Pontoscolex corethrurus is a pantropical geophagous endogeic earthworm species native to the Guyana Shield area in Northern Brazil. It is found throughout the country, mainly in disturbed ecosystems, although it also may inhabit natural ecosystems far from its original habitat, where it must be considered an exotic invasive species. Its distribution has been associated with a wide-ranging tolerance to different soil types and environments (Lavelle et al., 1987). However, the species is rare in agricultural fields of Northern Paraná State, Brazil. Therefore, the present study was undertaken to evaluate possible soil limitations for the colonization of this species at the Embrapa Soybean experiment station (Londrina, Brazil). P. corethrurus were incubated at 27ºC in the laboratory, using the Rhodic Hapludox from the station and different amounts of added organic matter (OM; composted city organic wastes) and sand. The following treatments were used: 100% soil; soil+2% (weight) OM; soil+4% OM; soil+10% OM; soil+10% sand; soil+30% sand; soil+60% sand. A potting mixture used in the greenhouse (70%soil, 20%sand, 10%OM) was also tested. Mean C content of the mixtures ranged from 1.2 to 4.3% and sand contents from 8 to 76%. Each container (10 cm square plastic box) received about 140g air-dry substrate, and water at 70-80% field capacity. Each box had 1 specimen and each treatment was replicated 14 times. The worms were cultivated for 395 d, and the substrates changed approximately every 20 d. After 1 yr, only 5 worms were alive in the soil+60% sand treatment and>9 individuals in the remaining treatments. A negative relationship was observed between sand content and earthworm biomass (R2 = 0,60). Worm biomass and soil C content showed a polynomial relationship (R2 = 0,89), with maximum values at 2.8% C. Positive linear correlations (R2 = 0,5) with soil P and Mg were also observed. Worms grew best in soil+2% OM, followed by soil+10% sand, soil+4% OM, soil 100%, and soil+30% sand. These results indicate that the soil from Embrapa Soybean is adequate for the development of P. corethrurus, and that some other factor must be limiting colonization by this species. Furthermore, a small increase in soil OM (from 2.3-2,6% C) could greatly benefit the populations of this species. MenosPontoscolex corethrurus is a pantropical geophagous endogeic earthworm species native to the Guyana Shield area in Northern Brazil. It is found throughout the country, mainly in disturbed ecosystems, although it also may inhabit natural ecosystems far from its original habitat, where it must be considered an exotic invasive species. Its distribution has been associated with a wide-ranging tolerance to different soil types and environments (Lavelle et al., 1987). However, the species is rare in agricultural fields of Northern Paraná State, Brazil. Therefore, the present study was undertaken to evaluate possible soil limitations for the colonization of this species at the Embrapa Soybean experiment station (Londrina, Brazil). P. corethrurus were incubated at 27ºC in the laboratory, using the Rhodic Hapludox from the station and different amounts of added organic matter (OM; composted city organic wastes) and sand. The following treatments were used: 100% soil; soil+2% (weight) OM; soil+4% OM; soil+10% OM; soil+10% sand; soil+30% sand; soil+60% sand. A potting mixture used in the greenhouse (70%soil, 20%sand, 10%OM) was also tested. Mean C content of the mixtures ranged from 1.2 to 4.3% and sand contents from 8 to 76%. Each container (10 cm square plastic box) received about 140g air-dry substrate, and water at 70-80% field capacity. Each box had 1 specimen and each treatment was replicated 14 times. The worms were cultivated for 395 d, and the substrates changed approximately ... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 02947naa a2200193 a 4500
001 1470761
005 2008-08-22
008 2006 bl --- 0-- u #d
100 1 $aBROWN, G. G.
245 $aGrowth of Pontoscolex corethrurus in an oxisol mixed or not with different amounts of sand and/or organic matter.
260 $c2006
520 $aPontoscolex corethrurus is a pantropical geophagous endogeic earthworm species native to the Guyana Shield area in Northern Brazil. It is found throughout the country, mainly in disturbed ecosystems, although it also may inhabit natural ecosystems far from its original habitat, where it must be considered an exotic invasive species. Its distribution has been associated with a wide-ranging tolerance to different soil types and environments (Lavelle et al., 1987). However, the species is rare in agricultural fields of Northern Paraná State, Brazil. Therefore, the present study was undertaken to evaluate possible soil limitations for the colonization of this species at the Embrapa Soybean experiment station (Londrina, Brazil). P. corethrurus were incubated at 27ºC in the laboratory, using the Rhodic Hapludox from the station and different amounts of added organic matter (OM; composted city organic wastes) and sand. The following treatments were used: 100% soil; soil+2% (weight) OM; soil+4% OM; soil+10% OM; soil+10% sand; soil+30% sand; soil+60% sand. A potting mixture used in the greenhouse (70%soil, 20%sand, 10%OM) was also tested. Mean C content of the mixtures ranged from 1.2 to 4.3% and sand contents from 8 to 76%. Each container (10 cm square plastic box) received about 140g air-dry substrate, and water at 70-80% field capacity. Each box had 1 specimen and each treatment was replicated 14 times. The worms were cultivated for 395 d, and the substrates changed approximately every 20 d. After 1 yr, only 5 worms were alive in the soil+60% sand treatment and>9 individuals in the remaining treatments. A negative relationship was observed between sand content and earthworm biomass (R2 = 0,60). Worm biomass and soil C content showed a polynomial relationship (R2 = 0,89), with maximum values at 2.8% C. Positive linear correlations (R2 = 0,5) with soil P and Mg were also observed. Worms grew best in soil+2% OM, followed by soil+10% sand, soil+4% OM, soil 100%, and soil+30% sand. These results indicate that the soil from Embrapa Soybean is adequate for the development of P. corethrurus, and that some other factor must be limiting colonization by this species. Furthermore, a small increase in soil OM (from 2.3-2,6% C) could greatly benefit the populations of this species.
700 1 $aMARTINS, P. T.
700 1 $aMATSUMURA, C.
700 1 $aSILVA, S. H. da
700 1 $aFERRARI, E.
700 1 $aKORASAKI, V.
700 1 $aPASINI, A.
773 $tIn: International Symposium on Earthworm Ecology, 8., 2006, Kraków. Abstracts... Kraków: Jagiellonian University, 2006. p. 61.
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