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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
24/02/1995 |
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Data da última atualização: |
15/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
REIFFERS, I.; FREIRE, A. de B. |
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Afiliação: |
I. REIFFERS, COMMISSION OF THE EUROPEAN COMMUNITY; ADELSON DE BARROS FREIRE, CNPAF. |
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Título: |
Production of doubled haploid rice plants (Oryza sativa L.) by anther culture. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v. 21, n. 2, p. 165-170, May 1990. |
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DOI: |
https://doi.org/10.1007/BF00033437 |
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Idioma: |
Inglês |
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Conteúdo: |
The results of anther culture of F 2 pollen issued from 23 single crosses are presented. A relation between the morphology of the panicle and the microspore stage was established. After cold-pretreatment (8 days at 4°C), the anthers were cultured on the callus-induction medium N6 supplemented with 1 mg 1-1 naphthaleneacetic acid. The calli were transferred to MS plant regeneration medium supplemented with 3mgl -~kinetin + 0.5 mg 1-' naphthaleneacetic acid. The induction frequency varied from 0.22% to 29% and the regeneration frequency from 0% to 144.4%, dependent upon the crosses used. On average, 27% of the plants obtained were albinos and 59% of the green plants underwent spontaneous chromosome doubling. Thirty- nine doubled haploid lines were evaluated and multiplied in the field. Lines with an excellent behaviour in upland culture conditions were selected from two crosses |
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Palavras-Chave: |
Doubled Haploid; Field Evaluation. |
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Thesagro: |
Arroz; Oryza Sativa; Tecido. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
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001 1199373
005 2022-09-15
008 1990 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/BF00033437$2DOI
100 1 $aREIFFERS, I.
245 $aProduction of doubled haploid rice plants (Oryza sativa L.) by anther culture.$h[electronic resource]
260 $c1990
520 $aThe results of anther culture of F 2 pollen issued from 23 single crosses are presented. A relation between the morphology of the panicle and the microspore stage was established. After cold-pretreatment (8 days at 4°C), the anthers were cultured on the callus-induction medium N6 supplemented with 1 mg 1-1 naphthaleneacetic acid. The calli were transferred to MS plant regeneration medium supplemented with 3mgl -~kinetin + 0.5 mg 1-' naphthaleneacetic acid. The induction frequency varied from 0.22% to 29% and the regeneration frequency from 0% to 144.4%, dependent upon the crosses used. On average, 27% of the plants obtained were albinos and 59% of the green plants underwent spontaneous chromosome doubling. Thirty- nine doubled haploid lines were evaluated and multiplied in the field. Lines with an excellent behaviour in upland culture conditions were selected from two crosses
650 $aArroz
650 $aOryza Sativa
650 $aTecido
653 $aDoubled Haploid
653 $aField Evaluation
700 1 $aFREIRE, A. de B.
773 $tPlant Cell, Tissue and Organ Culture$gv. 21, n. 2, p. 165-170, May 1990.
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Embrapa Arroz e Feijão (CNPAF) |
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