Data/hora: 12/06/2024 12:16:42

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	24/02/1995	
Data da última atualização: 	15/09/2022	
Autoria: 	REIFFERS, I.; FREIRE, A. de B.	
Afiliação: 	I. REIFFERS, COMMISSION OF THE EUROPEAN COMMUNITY; ADELSON DE BARROS FREIRE, CNPAF.	
Título: 	Production of doubled haploid rice plants (Oryza sativa L.) by anther culture.	
Ano de publicação: 	1990	
Fonte/Imprenta: 	Plant Cell, Tissue and Organ Culture, v. 21, n. 2, p. 165-170, May 1990.	
DOI: 	https://doi.org/10.1007/BF00033437	
Idioma: 	Inglês	
Conteúdo: 	The results of anther culture of F 2 pollen issued from 23 single crosses are presented. A relation between the morphology of the panicle and the microspore stage was established. After cold-pretreatment (8 days at 4°C), the anthers were cultured on the callus-induction medium N6 supplemented with 1 mg 1-1 naphthaleneacetic acid. The calli were transferred to MS plant regeneration medium supplemented with 3mgl -~kinetin + 0.5 mg 1-' naphthaleneacetic acid. The induction frequency varied from 0.22% to 29% and the regeneration frequency from 0% to 144.4%, dependent upon the crosses used. On average, 27% of the plants obtained were albinos and 59% of the green plants underwent spontaneous chromosome doubling. Thirty- nine doubled haploid lines were evaluated and multiplied in the field. Lines with an excellent behaviour in upland culture conditions were selected from two crosses	
Palavras-Chave: 	Doubled Haploid; Field Evaluation.	
Thesagro: 	Arroz; Oryza Sativa; Tecido.	
Categoria do assunto: 	F Plantas e Produtos de Origem Vegetal	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (8361-1) - UPC - AP - DD - 1990 -  - 1990 - DIS