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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Pecuária Sudeste. |
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Data corrente: |
27/05/2022 |
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Data da última atualização: |
20/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, M. M. de; NICIURA, S. C. M.; ROCHA, M. I. P.; PAN, Z.; ZHOU, H.; BRUSCADIN, J. J.; DINIZ, W. J. da S.; AFONSO, J.; OLIVEIRA, P. S. N. de; MOURÃO, G. B.; ZERLOTINI NETO, A.; COUTINHO, L. L.; KOLTES, J. E.; REGITANO, L. C. de A. |
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Afiliação: |
MARCELA MARIA DE SOUZA, IOWA STATE UNIVERSITY; SIMONE CRISTINA MEO NICIURA, CPPSE; MARINA IBELLI PEREIRA ROCHA, UFSCAR; ZHANGYUAN PAN, UNIVERSITY OF CALIFORNIA; HUAIJUN ZHOU, UNIVERSITY OF CALIFORNIA; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, AUBURN UNIVERSITY; JULIANA AFONSO; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; GERSON B. MOURÃO, ESALQ/USP; ADHEMAR ZERLOTINI NETO, CNPTIA; LUIZ LEHMANN COUTINHO, ESALQ/USP; JAMES E. KOLTES, IOWA STATE UNIVERSITY; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
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Título: |
DNA methylation may affect beef tenderness through signal transduction in Bos indicus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Epigenetics & Chromatin, v. 15, n. 1, p. 1-16, 2022. |
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DOI: |
https://doi.org/10.1186/s13072-022-00449-4 |
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Idioma: |
Inglês |
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Notas: |
Article number: 15. |
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Conteúdo: |
Abstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candi-date pathway associated with beef tenderness via methylation. MenosAbstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affec... Mostrar Tudo |
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Palavras-Chave: |
EBF3; Epigenoma; Epigenome; Força de cisalhamento; GNAS; Muscle; Músculo de nelore; Nelore; RRBS; Shear force. |
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Thesagro: |
Bos Indicus; Bos Taurus; Metilação; Músculo. |
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Thesaurus Nal: |
Cattle; DNA methylation; Methylation. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144144/1/DNA-MethylationMay.pdf
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Marc: |
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001 2144144
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024 7 $ahttps://doi.org/10.1186/s13072-022-00449-4$2DOI
100 1 $aSOUZA, M. M. de
245 $aDNA methylation may affect beef tenderness through signal transduction in Bos indicus.$h[electronic resource]
260 $c2022
500 $aArticle number: 15.
520 $aAbstract. Background:Beef tenderness is a complex trait of economic importance for the beef industry. Understanding the epigenetic mechanisms underlying this trait may help improve the accuracy of breeding programs. However, little is known about epigenetic effects on Bos taurus muscle and their implications in tenderness, and no studies have been conducted in Bos indicus.Results:Comparing methylation profile of Bos indicus skeletal muscle with contrasting beef tenderness at 14 days after slaughter, we identified differentially methylated cytosines and regions associated with this trait. Interestingly, muscle that became tender beef had higher levels of hypermethylation compared to the tough group. Enrichment analysis of predicted target genes suggested that differences in methylation between tender and tough beef may affect signal transduction pathways, among which G protein signaling was a key pathway. In addition, different meth-ylation levels were found associated with expression levels of GNAS, PDE4B, EPCAM and EBF3 genes. The differentially methylated elements correlated with EBF3 and GNAS genes overlapped CpG islands and regulatory elements. GNAS, a complex imprinted gene, has a key role on G protein signaling pathways. Moreover, both G protein signaling pathway and the EBF3 gene regulate muscle homeostasis, relaxation, and muscle cell-specificity.Conclusions:We present differentially methylated loci that may be of interest to decipher the epigenetic mecha-nisms affecting tenderness. Supported by the previous knowledge about regulatory elements and gene function, the methylation data suggests EBF3 and GNAS as potential candidate genes and G protein signaling as potential candi-date pathway associated with beef tenderness via methylation.
650 $aCattle
650 $aDNA methylation
650 $aMethylation
650 $aBos Indicus
650 $aBos Taurus
650 $aMetilação
650 $aMúsculo
653 $aEBF3
653 $aEpigenoma
653 $aEpigenome
653 $aForça de cisalhamento
653 $aGNAS
653 $aMuscle
653 $aMúsculo de nelore
653 $aNelore
653 $aRRBS
653 $aShear force
700 1 $aNICIURA, S. C. M.
700 1 $aROCHA, M. I. P.
700 1 $aPAN, Z.
700 1 $aZHOU, H.
700 1 $aBRUSCADIN, J. J.
700 1 $aDINIZ, W. J. da S.
700 1 $aAFONSO, J.
700 1 $aOLIVEIRA, P. S. N. de
700 1 $aMOURÃO, G. B.
700 1 $aZERLOTINI NETO, A.
700 1 $aCOUTINHO, L. L.
700 1 $aKOLTES, J. E.
700 1 $aREGITANO, L. C. de A.
773 $tEpigenetics & Chromatin$gv. 15, n. 1, p. 1-16, 2022.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| 5. |  | REGITANO, L. C. de A. Regras de segurança em laboratórios. In: REGITANO, L. C. de A.; NICIURA, S. C. M.; IBELLI, A. M. G.; GOUVEIA, J. J. de S. Protocolos em biologia molecular aplicada à produção animal. São Carlos: Embrapa Pecuária Sudeste, 2007. p. 1-2.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | REGITANO, L. C. de A. Quantificação em espectrofotômetro. In: REGITANO, L. C. de A.; NICIURA, S. C. M.; IBELLI, A. M. G.; GOUVEIA, J. J. de S. Protocolos em biologia molecular aplicada à produção animal. São Carlos: Embrapa Pecuária Sudeste, 2007. p. 12-13.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 630 | |
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