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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
09/12/2019 |
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Data da última atualização: |
09/12/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SOARES, M. P.; SHIROMA, L. S.; CARDOSO, I. L.; SAMPAIO, F. G.; JONSSON, C. M.; RANTIN, F. T.; QUEIROZ, S. C. do N. de. |
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Afiliação: |
M. P. SOARES, UFSCar; L. S. SHIROMA, Unicamp; I. L. CARDOSO, UFSCar; FERNANDA GARCIA SAMPAIO, CNPMA; CLAUDIO MARTIN JONSSON, CNPMA; F. T. RANTIN, UFSCar; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA. |
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Título: |
A rapid ultrasound-assisted dispersive liquid-liquid microextraction followed by liquid chromatography coupled to a mass spectrometry for determination of artemisinin in tilapia muscle. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON HIGH-PERFORMANCE LIQUID PHASE SEPARATIONS AND RELATED TECHNIQUES, 48., 2019, Milan. [Abstracts...] Milan: University of Milano-Bicocca, 2019. Ref. P 136. |
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Idioma: |
Português |
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Conteúdo: |
Intensive aquaculture represents stressful conditions for fish and can trigger diseases that negatively affect their performance and survival. Fish production requires greater control, with improved health conditions and food quality, concerned with consuming products free of therapeutic products and synthetic chemicals. The use of active ingredients extracted from plants is an alternative to improve the immune system of these animals, reducing problems with stress and the emergence of diseases. Artemisinin (ART), isolated from the Artemisia annua plant, is a promising and potent antimalarial drug that meets the challenge of resistant parasites. ART, an endoperoxide containing sesquiterpene lactone, is the main component responsible for the therapeutic effect. In aquaculture, herbal medicines have recently appeared in the framework of complementary and alternative medicines, because they have relatively lower cost and safety. However, careful may be taken with the application method considering environmental issues, since the administration directly in water causes an accelerated decomposition of compounds, restricting the additional use of the herbal medicine. Thinking of oral administration, many of these compounds are not completely metabolized by the animal?s organism, being eliminated in urine and feces. These residues can accumulate in the soil, undergo leaching, transported to the water sources and contaminate the aquatic compartment. In addition, these compounds may accumulate in medicated fish tissues for the purpose of preventing or treating infections, leading to problems in the consumption of these foods. Therefore, to evaluate the accumulation of residues in fish, an analytical method based on high performance liquid chromatography coupled to ion trap mass spectrometry (LC-MS/MS) was developed to quantify ART in tilapia muscles (Oreochromis niloticus). For sample preparation, 1 g of fish and 5 mL of disperser extractor acetonitrile were placed in tubes and vortexed for 1 minute. Then, 2 g of magnesium sulfate and 50 mg of C18 were added for lipid removal and again stirred in the vortex for 1 minute. The tubes were centrifuged at 3000 x g for 5 minutes. For ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME), 2 mL of the supernatant were transferred to centrifuge tubes, 2 mL of Milli-Q water was added and 250 ?L of toluene, for three times, was added, with a micro syringe. The tubes were left on ultrasound for 1 minute and centrifuged at 3,000 × g for 5 minutes. The supernatant was removed and dried with nitrogen, resuspended in 250 ?L of mobile phase, filtered with 0.22 ?m nylon filters and injected for LC-MS/MS analysis. The mobile phase, composed of ammonium acetate 10 mmol L-1:acetonitrile, 50:50 (v/v) was used. The proposed method was validated and successfully applied in pharmacokinetics studies. MenosIntensive aquaculture represents stressful conditions for fish and can trigger diseases that negatively affect their performance and survival. Fish production requires greater control, with improved health conditions and food quality, concerned with consuming products free of therapeutic products and synthetic chemicals. The use of active ingredients extracted from plants is an alternative to improve the immune system of these animals, reducing problems with stress and the emergence of diseases. Artemisinin (ART), isolated from the Artemisia annua plant, is a promising and potent antimalarial drug that meets the challenge of resistant parasites. ART, an endoperoxide containing sesquiterpene lactone, is the main component responsible for the therapeutic effect. In aquaculture, herbal medicines have recently appeared in the framework of complementary and alternative medicines, because they have relatively lower cost and safety. However, careful may be taken with the application method considering environmental issues, since the administration directly in water causes an accelerated decomposition of compounds, restricting the additional use of the herbal medicine. Thinking of oral administration, many of these compounds are not completely metabolized by the animal?s organism, being eliminated in urine and feces. These residues can accumulate in the soil, undergo leaching, transported to the water sources and contaminate the aquatic compartment. In addition, these compounds may ... Mostrar Tudo |
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Thesagro: |
Tilápia. |
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Thesaurus Nal: |
Artemisinin; Liquid chromatography. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/206510/1/RA-SampaioFG-et-al-HPLC2019-P136.pdf
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Marc: |
LEADER 03772nam a2200217 a 4500
001 2116384
005 2019-12-09
008 2019 bl uuuu u00u1 u #d
100 1 $aSOARES, M. P.
245 $aA rapid ultrasound-assisted dispersive liquid-liquid microextraction followed by liquid chromatography coupled to a mass spectrometry for determination of artemisinin in tilapia muscle.$h[electronic resource]
260 $aIn: INTERNATIONAL SYMPOSIUM ON HIGH-PERFORMANCE LIQUID PHASE SEPARATIONS AND RELATED TECHNIQUES, 48., 2019, Milan. [Abstracts...] Milan: University of Milano-Bicocca, 2019. Ref. P 136.$c2019
520 $aIntensive aquaculture represents stressful conditions for fish and can trigger diseases that negatively affect their performance and survival. Fish production requires greater control, with improved health conditions and food quality, concerned with consuming products free of therapeutic products and synthetic chemicals. The use of active ingredients extracted from plants is an alternative to improve the immune system of these animals, reducing problems with stress and the emergence of diseases. Artemisinin (ART), isolated from the Artemisia annua plant, is a promising and potent antimalarial drug that meets the challenge of resistant parasites. ART, an endoperoxide containing sesquiterpene lactone, is the main component responsible for the therapeutic effect. In aquaculture, herbal medicines have recently appeared in the framework of complementary and alternative medicines, because they have relatively lower cost and safety. However, careful may be taken with the application method considering environmental issues, since the administration directly in water causes an accelerated decomposition of compounds, restricting the additional use of the herbal medicine. Thinking of oral administration, many of these compounds are not completely metabolized by the animal?s organism, being eliminated in urine and feces. These residues can accumulate in the soil, undergo leaching, transported to the water sources and contaminate the aquatic compartment. In addition, these compounds may accumulate in medicated fish tissues for the purpose of preventing or treating infections, leading to problems in the consumption of these foods. Therefore, to evaluate the accumulation of residues in fish, an analytical method based on high performance liquid chromatography coupled to ion trap mass spectrometry (LC-MS/MS) was developed to quantify ART in tilapia muscles (Oreochromis niloticus). For sample preparation, 1 g of fish and 5 mL of disperser extractor acetonitrile were placed in tubes and vortexed for 1 minute. Then, 2 g of magnesium sulfate and 50 mg of C18 were added for lipid removal and again stirred in the vortex for 1 minute. The tubes were centrifuged at 3000 x g for 5 minutes. For ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME), 2 mL of the supernatant were transferred to centrifuge tubes, 2 mL of Milli-Q water was added and 250 ?L of toluene, for three times, was added, with a micro syringe. The tubes were left on ultrasound for 1 minute and centrifuged at 3,000 × g for 5 minutes. The supernatant was removed and dried with nitrogen, resuspended in 250 ?L of mobile phase, filtered with 0.22 ?m nylon filters and injected for LC-MS/MS analysis. The mobile phase, composed of ammonium acetate 10 mmol L-1:acetonitrile, 50:50 (v/v) was used. The proposed method was validated and successfully applied in pharmacokinetics studies.
650 $aArtemisinin
650 $aLiquid chromatography
650 $aTilápia
700 1 $aSHIROMA, L. S.
700 1 $aCARDOSO, I. L.
700 1 $aSAMPAIO, F. G.
700 1 $aJONSSON, C. M.
700 1 $aRANTIN, F. T.
700 1 $aQUEIROZ, S. C. do N. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Biblioteca |
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| Registros recuperados : 25 | |
| 4. |  | OBA, E. T.; SAMPAIO, F. G.; MONTAGNER, D.; SANTOS, L. R. B.; BOIJINK, C. de L.; RANTIN, F. T. Influência das práticas de manejo e do exercício físico moderado sobre o metabolismo de matrinxã, Brycon amazonicus. In: CONGRESSO BRASILEIRO DE PRODUÇÃO DE PEIXES NATIVOS DE ÁGUA DOCE, 2.; ENCONTRO MATO-GROSSENSE DE AQUICULTORES, 1., 2009, Cuiabá. [Resumos...]. Cuiabá: Embrapa: Aquabio, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
|    |
| 5. | | OBA, E. T.; SAMPAIO, F. G.; MONTAGNER, D.; SANTOS, L. R. B.; BOIJINK, C. L.; RANTIN, F. T. Influência das práticas de manejo e do exercício físico moderado sobre o metabolismo de matrinxã, Brycon amazonicus. In: CONGRESSO BRASILEIRO DE PRODUÇÃO DE PEIXES NATIVOS DE ÁGUA DOCE, 2.; ENCONTRO MATO-GROSSENSE DE AQUICULTORES, 1., 2009, Cuiabá. [Resumos...]. Cuiabá: Embrapa: Aquabio, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
|  |
| 6. | | FERRI, G. H.; CARDOSO, I. L.; GIL, J. A.; JONSSON, C. M.; RANTIN, F. T.; ISHIKAWA, M. M. Determination of acute median lethal concentration and sublethal effects on AChE activity of Gymnotus carapo (Teleostei: Gymnotidae) exposed to trichlorfon. Brazilian Journal of Veterinary Research And Animal Science, v. 7, n. 3, e169354, 2020. p. 1-8.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 7. | | SAMPAIO, F. G.; BOIJINK, C. de L.; SANTOS, L. R. B. dos; OBA, E. T.; KALININ, A. L.; LUIZ, A. J. B.; RANTIN, F. T. Antioxidant defenses and biochemical changes in the neotropical fish pacu, Piaractus mesopotamicus: responses to single and combined copper and hypercarbia exposure. Comparative Biochemistry and Physiology, Part C, New York, v. 156, n. 3-4, p. 178-186, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Meio Ambiente; Embrapa Pesca e Aquicultura. |
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| 8. | | SAMPAIO, F. G.; BOIJINK, C. de L.; SANTOS, L. R. B. dos; OBA, E. T.; KALININ, A. L.; LUIZ, A. J. B.; RANTIN, F. T. Antioxidant defenses and biochemical changes in the neotropical fish pacu, Piaractus mesopotamicus: responses to single and combined copper and hypercarbia exposure. Comparative Biochemistry and Physiology, Part C, New York, v. 156, n. 3-4, p. 178-186, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amapá. |
| |
| 9. | | SAMPAIO, F. G.; BOIJINK, C. de L.; OBA, E. T.; SANTOS, L. R. B. dos; KALININ, A. L.; LUIZ, A. J. B.; RANTIN, F. T. Antioxidant defenses and biochemical changes in pacu in response to single and combined copper and hypercapnia exposure. In: SIMPÓSIO INTERNACIONAL DE NUTRIÇÃO E SAÚDE DE PEIXES, 4., 2011, Botucatu. [Trabalhos apresentados...]. Botucatu: FMVZ: UNESP, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
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| 10. | | SAMPAIO, F. G.; BOIJINK, C. de L.; OBA, E. T.; SANTOS, L. R. B. dos; KALININ, A. L.; LUIZ, A. J. B.; RANTIN, F. T. Antioxidant defenses and biochemical changes in pacu in response to single and combined copper and hypercapnia exposure. In: SIMPÓSIO INTERNACIONAL DE NUTRIÇÃO E SAÚDE DE PEIXES, 4., 2011, Botucatu. [Trabalhos apresentados...]. Botucatu: FMVZ : UNESP, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 11. | | SAMPAIO, F. G.; BOIJINK, C. de L.; TIE OBA, E.; SANTOS, L. R. B. dos; KALININ, A. L.; LUIZ, A. J. B.; RANTIN, F. T. Antioxidant defenses and biochemical changes in pacu in response to single and combined copper and hypercapnia exposure. In: SIMPÓSIO INTERNACIONAL DE NUTRIÇÃO E SAÚDE DE PEIXES, 4., 2011, Botucatu. [Trabalhos apresentados...]. Botucatu: FMVZ: UNESP, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Pesca e Aquicultura. |
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| 12. | | SAMPAIO, F. G.; BOIJINK, C. de L.; SANTOS, L. R. B. DOS; YOSHIOKA, E. T. O.; KALININ, A. L.; RANTIN, F. T. The combined effect of cooper and low pH on antioxidant defenses and biochemical parameters in neotropical fish pacu, Piaractus mesopotamicus (Holmberg, 1887). Ecotoxicology, v. 19, n. 5, p. 963-976, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amapá; Embrapa Amazônia Ocidental. |
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| 13. | | LOPES, J. M.; BOIJINK, C. de L.; FLORINDO, L. H.; LEITE, C. A. C.; KALININ, A. L.; MILSOM, W. K.; RANTIN, F. T. Hypoxic cardiorespiratory reflexes in the facultative air-breathing fish jeju (Hoplerythrinus uniataeniatus): role of branchial O2 chemoreceptors. Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, v. 180, p. 797-811, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 14. | | SOARES, M. P.; SHIROMA, L. S.; CARDOSO, I. L.; SAMPAIO, F. G.; JONSSON, C. M.; RANTIN, F. T.; QUEIROZ, S. C. do N. de. A rapid ultrasound-assisted dispersive liquid-liquid microextraction followed by liquid chromatography coupled to a mass spectrometry for determination of artemisinin in tilapia muscle. In: INTERNATIONAL SYMPOSIUM ON HIGH-PERFORMANCE LIQUID PHASE SEPARATIONS AND RELATED TECHNIQUES, 48., 2019, Milan. [Abstracts...] Milan: University of Milano-Bicocca, 2019. Ref. P 136.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 15. | | OBA, E. T.; MONTAGNER, D.; BOIJINK, C. de L.; SANTOS, L. R. B. dos; SAMPAIO, F. G.; MARIANO, W. dos S.; RANTIN, F. T. Respostas fisiológicas de carpa comum, Cyprinus carpio, submetida à natação forçada. In: CONGRESSO BRASILEIRO DE ENGENHARIA DE PESCA, 16., 2009, Natal. A importância da engenharia de pesca na produção de alimentos de recursos aquáticos. p. 84-90.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
| |
| 16. | | OBA, E. T.; MONTAGNER, D.; BOIJINK, C. de L.; SANTOS, L. R. B. dos; SAMPAIO, F. G.; MARIANO, W. dos S.; RANTIN, F. T. Respostas fisiológicas de carpa comum, Cyprinus carpio, submetida à natação forçada. In: CONGRESSO BRASILEIRO DE ENGENHARIA DE PESCA, 16., 2009, Natal. A importância da engenharia de pesca na produção de alimentos de recursos aquáticos. p. 84-90.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amapá. |
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| 17. | | CARDOSO, I. L.; SOARES, M. P.; DE ANGELIS, C.; FERRI, G. H.; ISHIKAWA, M. M.; JONSSON, C. M.; RANTIN, F. T.; SAMPAIO, F. G. Physiological and biochemical responses of Nile tilapia (Oreochromis niloticus) to acute trichlorfon exposure. International Aquatic Research, v. 12, n. 4, p. 243-253, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 18. | | REZENDE, R. A. E. de; SOARES, M. P.; SAMPAIO, F. G.; CARDOSO, I. L.; ISHIKAWA, M. M.; DALLAGO, B. S. L.; RANTIN, F. T.; DUARTE, M. C. T. Phytobiotics blend as a dietary supplement for Nile tilapia health improvement. Fish & Shellfish Immunology, V. 114, p. 293-300, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Meio Ambiente. |
| |
| 19. | | SAMPAIO, F. G.; BOIJINK, C. de L.; TIE OBA, E.; SANTOS, L. R. B. dos; KALININ, A. L.; LOSEKANN, M. E.; LUIZ, A. J. B.; RANTIN, F. T. Stocking density and copper effects on the antioxidant defenses and hematological paramenters of pacu. In: SIMPÓSIO INTERNACIONAL DE NUTRIÇÃO E SAÚDE DE PEIXES, 4., 2011, Botucatu. [Trabalhos apresentados...]. Botucatu: FMVZ: UNESP, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Pesca e Aquicultura. |
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| 20. | | SAMPAIO, F. G.; BOIJINK, C. de L.; OBA, E. T.; SANTOS, L. R. B. dos; KALININ, A. L.; LOSEKANN, M. E.; LUIZ, A. J. B.; RANTIN, F. T. Stocking density and copper effects on the antioxidant defenses and hematological parameters of pacu. In: SIMPÓSIO INTERNACIONAL DE NUTRIÇÃO E SAÚDE DE PEIXES, 4., 2011, Botucatu. [Trabalhos apresentados...]. Botucatu: FMVZ : UNESP, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 25 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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