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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
18/11/1999 |
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Data da última atualização: |
28/03/2017 |
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Autoria: |
RAMSAY, A. J. |
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Afiliação: |
Cawthron Institute (Nelson, New Zealand). |
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Título: |
Direct counts of bacteria by a modified acridine orange method in relation to their heterotrophic activity. |
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Ano de publicação: |
1978 |
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Fonte/Imprenta: |
New Zealand Journal of Marine and Freshwater Research, Wellington, v.12, n.3, p.265-269, sept., 1978. |
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Idioma: |
Inglês |
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Conteúdo: |
Modifying the acridine orange staining procedure used for direct counting of aquatic bacteria, by staining the bacteria after filtering instead of in solution, gave bright fluorescence and eliminated the need for filtering stained samples. Millipore, Sartorius, and Nucleopore filters all gave good results with the staining method. Counts on the Nucleopore polycarbonate filters were about twice as high as on Millipore and Sartorius cellulose filters. Bacteria were counted and their heterophic acitivity measured as uptake and respiration of 14C-glucose in 111 freshwater and marine samples. For standing waters which were not grossly polluted, bacterial counts per millilitre, using Millipore filters, varied between narrow limits (1.16-17.4 X 10 3), but the mean heteroptrophic activity per bacterium showed a much wider range of values (0.6-111 X 10 -11 ug.h-1). |
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Palavras-Chave: |
Modificacao; Modification. |
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Thesagro: |
Bactéria. |
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Thesaurus Nal: |
acridine orange. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01415naa a2200169 a 4500
001 1799586
005 2017-03-28
008 1978 bl --- 0-- u #d
100 1 $aRAMSAY, A. J.
245 $aDirect counts of bacteria by a modified acridine orange method in relation to their heterotrophic activity.
260 $c1978
520 $aModifying the acridine orange staining procedure used for direct counting of aquatic bacteria, by staining the bacteria after filtering instead of in solution, gave bright fluorescence and eliminated the need for filtering stained samples. Millipore, Sartorius, and Nucleopore filters all gave good results with the staining method. Counts on the Nucleopore polycarbonate filters were about twice as high as on Millipore and Sartorius cellulose filters. Bacteria were counted and their heterophic acitivity measured as uptake and respiration of 14C-glucose in 111 freshwater and marine samples. For standing waters which were not grossly polluted, bacterial counts per millilitre, using Millipore filters, varied between narrow limits (1.16-17.4 X 10 3), but the mean heteroptrophic activity per bacterium showed a much wider range of values (0.6-111 X 10 -11 ug.h-1).
650 $aacridine orange
650 $aBactéria
653 $aModificacao
653 $aModification
773 $tNew Zealand Journal of Marine and Freshwater Research, Wellington$gv.12, n.3, p.265-269, sept., 1978.
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Registro original: |
Embrapa Pantanal (CPAP) |
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