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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
31/03/2016 |
Data da última atualização: |
26/01/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
FREITAS NETO, M.; PEREIRA, T. N. S.; GERONIMO, I. G. C.; AZEVEDO, A. O. N.; RAMOS, S. R. R.; PEREIRA, M. G. |
Título: |
Coconut genome size determined by flow cytometry: tall versus dwarf types. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 15, n. 1, february, 2016. |
DOI: |
http://dx.doi.org/10.4238/gmr.15017470 |
Idioma: |
Português |
Conteúdo: |
Coconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf type did not evolve from the Tall type. MenosCoconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf ty... Mostrar Tudo |
Palavras-Chave: |
C-value; Coconut accessions; Cocos nucifera L; DNA content; Palm tree. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141883/1/gmr7470.pdf
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Marc: |
LEADER 02294naa a2200253 a 4500 001 2042274 005 2018-01-26 008 2016 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.4238/gmr.15017470$2DOI 100 1 $aFREITAS NETO, M. 245 $aCoconut genome size determined by flow cytometry$btall versus dwarf types.$h[electronic resource] 260 $c2016 520 $aCoconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf type did not evolve from the Tall type. 653 $aC-value 653 $aCoconut accessions 653 $aCocos nucifera L 653 $aDNA content 653 $aPalm tree 700 1 $aPEREIRA, T. N. S. 700 1 $aGERONIMO, I. G. C. 700 1 $aAZEVEDO, A. O. N. 700 1 $aRAMOS, S. R. R. 700 1 $aPEREIRA, M. G. 773 $tGenetics and Molecular Research$gv. 15, n. 1, february, 2016.
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Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
12/01/2024 |
Data da última atualização: |
12/01/2024 |
Tipo da produção científica: |
Artigo de Divulgação na Mídia |
Autoria: |
PALHARES, J. C. P. |
Afiliação: |
JULIO CESAR PASCALE PALHARES, CPPSE. |
Título: |
Avaliação do impacto ambiental na atividade leiteira. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Milkpoint, 03 nov. 2023. |
Idioma: |
Português |
Conteúdo: |
A avaliação dos impactos ambientais na atividade leiteira é o ponto inicial para implantação de um sistema de gestão ambiental da propriedade ou, como preferem os “modistas”, para um sistema ASG (Ambiente, Sociedade e Governança). |
Palavras-Chave: |
Atividade leiteira; Impactos ambientais; Qualidade dos recursos naturais; Quantidade dos recursos naturais. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1160782/1/AvaliacaoImpactoAmbientalAtividade.pdf
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Marc: |
LEADER 00725nam a2200157 a 4500 001 2160782 005 2024-01-12 008 2023 bl uuuu u00u1 u #d 100 1 $aPALHARES, J. C. P. 245 $aAvaliação do impacto ambiental na atividade leiteira.$h[electronic resource] 260 $aMilkpoint, 03 nov. 2023.$c2023 520 $aA avaliação dos impactos ambientais na atividade leiteira é o ponto inicial para implantação de um sistema de gestão ambiental da propriedade ou, como preferem os “modistas”, para um sistema ASG (Ambiente, Sociedade e Governança). 653 $aAtividade leiteira 653 $aImpactos ambientais 653 $aQualidade dos recursos naturais 653 $aQuantidade dos recursos naturais
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